##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AviII BalI BamHI BanII BbeI
BbrPI BbvCI BciVI BclI BcnI
BfrI BglI BmyI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NciI NcoI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 869 GTAGAC GT^[AC][GT]AC
AccII 1166 CGCG CG^CG
AseI 1476 ATTAAT AT^TAAT
AsnI 1476 ATTAAT AT^TAAT
AvrII 1009 CCTAGG C^CTAGG
BbsI 131 GAAGAC GAAGAC(2/6)
BcgI 7 CGACGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 106 AGATCT A^GATCT
BlnI 1009 CCTAGG C^CTAGG
BlpI 1401 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1401 GCTAAGC GC^T[ACGT]AGC
BpuAI 131 GAAGAC GAAGAC(2/6)
BseRI 27 CTCCTC GAGGAG(10/8)
BsmAI 1029 GTCTC GTCTC(1/5)
Bsp1407I 254 TGTACA T^GTACA
BsrDI 992 GCAATG GCAATG(2/0)
BsrGI 254 TGTACA T^GTACA
BstAPI 175 GCATGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1229 GGTGACC G^GT[ACGT]ACC
BstPI 1229 GGTGACC G^GT[ACGT]ACC
BstUI 1166 CGCG CG^CG
BstYI 106 AGATCT [GA]^GATC[CT]
CelII 1401 GCTAAGC GC^T[ACGT]AGC
DraIII 216 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 339 GACAGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 596 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1229 GGTGACC G^GT[ACGT]ACC
HaeIII 591 GGCC GG^CC
HapII 971 CCGG C^CGG
HpaII 971 CCGG C^CGG
MflI 106 AGATCT [GA]^GATC[CT]
MslI 1243 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 971 CCGG C^CGG
MvnI 1166 CGCG CG^CG
NdeI 1430 CATATG CA^TATG
NheI 113 GCTAGC G^CTAGC
NspI 175 GCATGC [GA]CATG^[CT]
PleI 346 GAGTC GAGTC(4/5)
PmeI 997 GTTTAAAC GTTT^AAAC
PshBI 1476 ATTAAT AT^TAAT
SphI 175 GCATGC GCATG^C
SspBI 254 TGTACA T^GTACA
TaqI 6 TCGA T^CGA
TthHB8I 6 TCGA T^CGA
XcmI 455 CCAAATGTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 106 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 155 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
721 GAATTTATTC
AvaI 984 CCCGAG C^[CT]CG[GA]G
1176 CCCGAG
AvaII 1014 GGACC G^G[AT]CC
1169 GGACC
BanI 434 GGTGCC G^G[CT][GA]CC
1238 GGTGCC
BsaAI 822 CACGTA [CT]AC^GT[GA]
1104 TACGTG
BsgI 1004 CTGCAC GTGCAG(16/14)
182 GTGCAG
BsiYI 566 CCTTGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
596 CCTGGAACAGG
BslI 566 CCTTGGAGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
596 CCTGGAACAGG
BsmFI 1013 GGGAC GGGAC(10/14)
1265 GTCCC
BsoBI 984 CCCGAG C^[CT]CG[GA]G
1176 CCCGAG
Cac8I 113 GCTAGC GC[ACGT]^[ACGT]GC
175 GCATGC
CfoI 1 GCGC GCG^C
125 GCGC
DraI 998 TTTAAA TTT^AAA
1154 TTTAAA
HhaI 1 GCGC GCG^C
125 GCGC
HinP1I 1 GCGC G^CGC
125 GCGC
SfaNI 1109 GATGC GCATC(5/9)
1421 GATGC
XmnI 155 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
721 GAATTTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BpmI BsaJI
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI