##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI BbrPI BcgI BciVI
BfrI BglII BsaAI BsaBI BsiEI
BsiWI BssHII BstAPI CpoI DraI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV FseI HindIII HpaI KpnI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SfiI SgrAI SnaBI SpeI
SspI SwaI TfiI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 1761 TCCGGA T^CCGGA
AhdI 1024 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1530 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1024 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 803 TGCGCA TGC^GCA
BbeI 783 GGCGCC GGCGC^C
BclI 2363 TGATCA T^GATCA
BglI 1818 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 1761 TCCGGA T^CCGGA
Bsp1407I 3487 TGTACA T^GTACA
BspDI 1993 ATCGAT AT^CGAT
BspEI 1761 TCCGGA T^CCGGA
BspHI 2472 TCATGA T^CATGA
BspMI 3166 GCAGGT ACCTGC(4/8)
BsrFI 1914 GCCGGC [GA]^CCGG[CT]
BsrGI 3487 TGTACA T^GTACA
BssSI 649 CTCGTG CACGAG(-5/-1)
Bst1107I 2458 GTATAC GTA^TAC
BstBI 1610 TTCGAA TT^CGAA
BstEII 2533 GGTGACC G^GT[ACGT]ACC
BstPI 2533 GGTGACC G^GT[ACGT]ACC
BstZ17I 2458 GTATAC GTA^TAC
Cfr10I 1914 GCCGGC [GA]^CCGG[CT]
ClaI 1993 ATCGAT AT^CGAT
DraIII 2528 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1024 GACATCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2533 GGTGACC G^GT[ACGT]ACC
EcoT22I 2408 ATGCAT ATGCA^T
FbaI 2363 TGATCA T^GATCA
FspI 803 TGCGCA TGC^GCA
HaeII 783 GGCGCC [GA]GCGC^[CT]
KasI 783 GGCGCC G^GCGCC
MroI 1761 TCCGGA T^CCGGA
NaeI 1914 GCCGGC GCC^GGC
NarI 783 GGCGCC GG^CGCC
NgoMIV 1914 GCCGGC G^CCGGC
NsiI 2408 ATGCAT ATGCA^T
NspV 1610 TTCGAA TT^CGAA
PaeR7I 2180 CTCGAG C^TCGAG
RcaI 2472 TCATGA T^CATGA
SacI 3466 GAGCTC GAGCT^C
SalI 1390 GTCGAC G^TCGAC
SapI 2166 GAAGAGC GCTCTTC(1/4)
SbfI 1331 CCTGCAGG CCTGCA^GG
ScaI 1697 AGTACT AGT^ACT
SfoI 783 GGCGCC GGC^GCC
SfuI 1610 TTCGAA TT^CGAA
Sse8387I 1331 CCTGCAGG CCTGCA^GG
SspBI 3487 TGTACA T^GTACA
XhoI 2180 CTCGAG C^TCGAG
XmnI 1530 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 811 GACGTC GACGT^C
2289 GACGTC
AccII 1745 CGCG CG^CG
3422 CGCG
AcsI 354 GAATTT [GA]^AATT[CT]
944 AAATTT
AflIII 1245 ACATGT A^C[GA][CT]GT
3443 ACATGT
ApaI 323 GGGCCC GGGCC^C
3088 GGGCCC
ApoI 354 GAATTT [GA]^AATT[CT]
944 AAATTT
AvrII 1836 CCTAGG C^CTAGG
2494 CCTAGG
BalI 107 TGGCCA TGG^CCA
2327 TGGCCA
BbsI 760 GAAGAC GAAGAC(2/6)
2921 GAAGAC
BlnI 1836 CCTAGG C^CTAGG
2494 CCTAGG
BlpI 1582 GCTGAGC GC^T[ACGT]AGC
2007 GCTGAGC
Bpu1102I 1582 GCTGAGC GC^T[ACGT]AGC
2007 GCTGAGC
BpuAI 760 GAAGAC GAAGAC(2/6)
2921 GAAGAC
BsaWI 1427 ACCGGA [AT]^CCGG[AT]
1761 TCCGGA
BspLU11I 1245 ACATGT A^CATGT
3443 ACATGT
BsrBI 1819 CCGCTC CCGCTC(-3/-3)
12 GAGCGG
BsrDI 111 CATTGC GCAATG(2/0)
2351 GCAATG
BstUI 1745 CGCG CG^CG
3422 CGCG
CelII 1582 GCTGAGC GC^T[ACGT]AGC
2007 GCTGAGC
DrdI 717 GACATGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
763 GACAGCGTGGTC
EcoNI 1251 CCTGGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1469 CCTACAGAAGG
MluNI 107 TGGCCA TGG^CCA
2327 TGGCCA
MscI 107 TGGCCA TGG^CCA
2327 TGGCCA
MvnI 1745 CGCG CG^CG
3422 CGCG
NcoI 2666 CCATGG C^CATGG
3297 CCATGG
SexAI 260 ACCAGGT A^CC[AT]GGT
2529 ACCTGGT
SphI 2012 GCATGC GCATG^C
2406 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BamHI BanI BanII BbvCI
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI