##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AviII
BalI BamHI BbeI BbrPI BbsI
BpuAI BsaAI BsaHI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI DraII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I FseI FspI HaeII Hin1I
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RcaI SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AflII 1842 CTTAAG C^TTAAG
AgeI 2624 ACCGGT A^CCGGT
Alw44I 329 GTGCAC G^TGCAC
AlwNI 1360 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 329 GTGCAC G^TGCAC
AseI 1317 ATTAAT AT^TAAT
AsnI 1317 ATTAAT AT^TAAT
AvaI 3432 CTCGAG C^[CT]CG[GA]G
AvrII 1121 CCTAGG C^CTAGG
BbvCI 2581 GCTGAGG CCTCAGC(-5/-2)
BcgI 3196 GCAAATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1842 CTTAAG C^TTAAG
BglI 49 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 730 AGATCT A^GATCT
BlnI 1121 CCTAGG C^CTAGG
BlpI 1227 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1227 GCTGAGC GC^T[ACGT]AGC
BsaBI 3012 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 465 GGTCTC GGTCTC(1/5)
BsiEI 8 CGGTCG CG[GA][CT]^CG
BsmBI 696 GAGACG CGTCTC(1/5)
BsoBI 3432 CTCGAG C^[CT]CG[GA]G
BspMI 1183 ACCTGC ACCTGC(4/8)
BsrBI 1230 GAGCGG CCGCTC(-3/-3)
BsrFI 2624 ACCGGT [GA]^CCGG[CT]
BstEII 379 GGTGACC G^GT[ACGT]ACC
BstPI 379 GGTGACC G^GT[ACGT]ACC
CelII 1227 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2624 ACCGGT [GA]^CCGG[CT]
CpoI 3 CGGTCCG CG^G[AT]CCG
DraIII 21 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2713 CCACGG C^C[GA][CT]GG
EcoNI 1184 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 379 GGTGACC G^GT[ACGT]ACC
EcoRI 3043 GAATTC G^AATTC
EcoRV 2094 GATATC GAT^ATC
HincII 3241 GTTAAC GT[CT]^[GA]AC
HindII 3241 GTTAAC GT[CT]^[GA]AC
HindIII 955 AAGCTT A^AGCTT
HpaI 3241 GTTAAC GTT^AAC
MamI 3012 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PaeR7I 3432 CTCGAG C^TCGAG
PinAI 2624 ACCGGT A^CCGGT
PshAI 2884 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1317 ATTAAT AT^TAAT
RsrII 3 CGGTCCG CG^G[AT]CCG
SapI 3295 GAAGAGC GCTCTTC(1/4)
XbaI 1069 TCTAGA T^CTAGA
XcmI 1315 CCATTAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3432 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 2082 GTAGAC GT^[AC][GT]AC
2281 GTAGAC
BanI 1180 GGCACC G^G[CT][GA]CC
2985 GGCACC
BclI 2706 TGATCA T^GATCA
3249 TGATCA
BsaWI 6 TCCGGT [AT]^CCGG[AT]
2624 ACCGGT
BsmI 430 GAATGC GAATGC(1/-1)
652 GAATGC
BspLU11I 2078 ACATGT A^CATGT
3119 ACATGT
BsrDI 354 CATTGC GCAATG(2/0)
2053 CATTGC
BssHII 70 GCGCGC G^CGCGC
106 GCGCGC
BstAPI 2939 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2973 GCATCTATTGC
BstXI 161 CCAGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
384 CCAGTGACTTGG
BstYI 730 AGATCT [GA]^GATC[CT]
3024 AGATCC
EcoT22I 1505 ATGCAT ATGCA^T
2462 ATGCAT
FbaI 2706 TGATCA T^GATCA
3249 TGATCA
HgaI 82 GACGC GACGC(5/10)
13 GCGTC
MflI 730 AGATCT [GA]^GATC[CT]
3024 AGATCC
MslI 743 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1316 CATTAATGTG
NsiI 1505 ATGCAT ATGCA^T
2462 ATGCAT
SacI 349 GAGCTC GAGCT^C
1424 GAGCTC
ScaI 3436 AGTACT AGT^ACT
3491 AGTACT
XhoII 730 AGATCT [GA]^GATC[CT]
3024 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaII
BanII BbvI BciVI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I