################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AviII BalI BamHI BbeI BbrPI BbsI BpuAI BsaAI BsaHI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI DraII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I FseI FspI HaeII Hin1I KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PflMI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 1842 CTTAAG C^TTAAG AgeI 2624 ACCGGT A^CCGGT Alw44I 329 GTGCAC G^TGCAC AlwNI 1360 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 329 GTGCAC G^TGCAC AseI 1317 ATTAAT AT^TAAT AsnI 1317 ATTAAT AT^TAAT AvaI 3432 CTCGAG C^[CT]CG[GA]G AvrII 1121 CCTAGG C^CTAGG BbvCI 2581 GCTGAGG CCTCAGC(-5/-2) BcgI 3196 GCAAATCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1842 CTTAAG C^TTAAG BglI 49 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 730 AGATCT A^GATCT BlnI 1121 CCTAGG C^CTAGG BlpI 1227 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1227 GCTGAGC GC^T[ACGT]AGC BsaBI 3012 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 465 GGTCTC GGTCTC(1/5) BsiEI 8 CGGTCG CG[GA][CT]^CG BsmBI 696 GAGACG CGTCTC(1/5) BsoBI 3432 CTCGAG C^[CT]CG[GA]G BspMI 1183 ACCTGC ACCTGC(4/8) BsrBI 1230 GAGCGG CCGCTC(-3/-3) BsrFI 2624 ACCGGT [GA]^CCGG[CT] BstEII 379 GGTGACC G^GT[ACGT]ACC BstPI 379 GGTGACC G^GT[ACGT]ACC CelII 1227 GCTGAGC GC^T[ACGT]AGC Cfr10I 2624 ACCGGT [GA]^CCGG[CT] CpoI 3 CGGTCCG CG^G[AT]CCG DraIII 21 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2713 CCACGG C^C[GA][CT]GG EcoNI 1184 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 379 GGTGACC G^GT[ACGT]ACC EcoRI 3043 GAATTC G^AATTC EcoRV 2094 GATATC GAT^ATC HincII 3241 GTTAAC GT[CT]^[GA]AC HindII 3241 GTTAAC GT[CT]^[GA]AC HindIII 955 AAGCTT A^AGCTT HpaI 3241 GTTAAC GTT^AAC MamI 3012 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PaeR7I 3432 CTCGAG C^TCGAG PinAI 2624 ACCGGT A^CCGGT PshAI 2884 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1317 ATTAAT AT^TAAT RsrII 3 CGGTCCG CG^G[AT]CCG SapI 3295 GAAGAGC GCTCTTC(1/4) XbaI 1069 TCTAGA T^CTAGA XcmI 1315 CCATTAATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 3432 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 2082 GTAGAC GT^[AC][GT]AC 2281 GTAGAC BanI 1180 GGCACC G^G[CT][GA]CC 2985 GGCACC BclI 2706 TGATCA T^GATCA 3249 TGATCA BsaWI 6 TCCGGT [AT]^CCGG[AT] 2624 ACCGGT BsmI 430 GAATGC GAATGC(1/-1) 652 GAATGC BspLU11I 2078 ACATGT A^CATGT 3119 ACATGT BsrDI 354 CATTGC GCAATG(2/0) 2053 CATTGC BssHII 70 GCGCGC G^CGCGC 106 GCGCGC BstAPI 2939 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2973 GCATCTATTGC BstXI 161 CCAGTTTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 384 CCAGTGACTTGG BstYI 730 AGATCT [GA]^GATC[CT] 3024 AGATCC EcoT22I 1505 ATGCAT ATGCA^T 2462 ATGCAT FbaI 2706 TGATCA T^GATCA 3249 TGATCA HgaI 82 GACGC GACGC(5/10) 13 GCGTC MflI 730 AGATCT [GA]^GATC[CT] 3024 AGATCC MslI 743 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1316 CATTAATGTG NsiI 1505 ATGCAT ATGCA^T 2462 ATGCAT SacI 349 GAGCTC GAGCT^C 1424 GAGCTC ScaI 3436 AGTACT AGT^ACT 3491 AGTACT XhoII 730 AGATCT [GA]^GATC[CT] 3024 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanII BbvI BciVI BcnI BfaI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I