##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaII AviII
BamHI BbrPI BcgI BciVI BfrI
BsaWI BseAI BsiHKAI BsiWI Bsp1407I
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstZ17I Cfr10I CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoO65I EcoRV
FseI FspI HgaI HindIII HpaI
KpnI KspI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NspI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SgrAI
SmaI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AcyI 97 GGCGCC G[GA]^CG[CT]C
AlwNI 924 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2672 CCTGAGG CC^T[ACGT]AGG
Asp700I 1268 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 2582 CCCGAG C^[CT]CG[GA]G
AvrII 1024 CCTAGG C^CTAGG
BanII 110 GAGCCC G[GA]GC[CT]^C
BbeI 97 GGCGCC GGCGC^C
BbsI 2126 GAAGAC GAAGAC(2/6)
BbvCI 1550 GCTGAGG CCTCAGC(-5/-2)
BglI 2743 GCCAGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2693 AGATCT A^GATCT
BlnI 1024 CCTAGG C^CTAGG
BmyI 110 GAGCCC G[AGT]GC[ACT]^C
BpuAI 2126 GAAGAC GAAGAC(2/6)
BsaAI 2080 TACGTA [CT]AC^GT[GA]
BsaBI 1981 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 97 GGCGCC G[GA]^CG[CT]C
BseRI 704 GAGGAG GAGGAG(10/8)
BsiEI 490 CGATCG CG[GA][CT]^CG
BsmBI 866 CGTCTC CGTCTC(1/5)
BsoBI 2582 CCCGAG C^[CT]CG[GA]G
Bsp1286I 110 GAGCCC G[AGT]GC[ACT]^C
BspDI 1966 ATCGAT AT^CGAT
BsrBI 1726 CCGCTC CCGCTC(-3/-3)
BstBI 960 TTCGAA TT^CGAA
BstXI 1003 CCAAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2672 CCTGAGG CC^T[ACGT]AGG
ClaI 1966 ATCGAT AT^CGAT
DsaI 105 CCATGG C^C[GA][CT]GG
Eco81I 2672 CCTGAGG CC^T[ACGT]AGG
Hin1I 97 GGCGCC G[GA]^CG[CT]C
KasI 97 GGCGCC G^GCGCC
MamI 1981 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 9 CAGCTG C[AC]G^C[GT]G
NarI 97 GGCGCC GG^CGCC
NcoI 105 CCATGG C^CATGG
NspV 960 TTCGAA TT^CGAA
PacI 2757 TTAATTAA TTAAT^TAA
PleI 2475 GAGTC GAGTC(4/5)
PvuI 490 CGATCG CGAT^CG
PvuII 9 CAGCTG CAG^CTG
ScaI 1742 AGTACT AGT^ACT
SfoI 97 GGCGCC GGC^GCC
SfuI 960 TTCGAA TT^CGAA
SnaBI 2080 TACGTA TAC^GTA
XcmI 417 CCACTGTGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1268 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
BalI 524 TGGCCA TGG^CCA
1453 TGGCCA
BanI 97 GGCGCC G^G[CT][GA]CC
2591 GGCACC
BclI 985 TGATCA T^GATCA
2514 TGATCA
BcnI 71 CCCGG CC^[GC]GG
94 CCCGG
BlpI 1562 GCTCAGC GC^T[ACGT]AGC
2256 GCTAAGC
Bpu1102I 1562 GCTCAGC GC^T[ACGT]AGC
2256 GCTAAGC
BsmI 856 GAATGC GAATGC(1/-1)
1259 GCATTC
CelII 1562 GCTCAGC GC^T[ACGT]AGC
2256 GCTAAGC
Cfr13I 64 GGCCC G^G[ACGT]CC
2573 GGCCC
EaeI 524 TGGCCA [CT]^GGCC[GA]
1453 TGGCCA
EcoNI 175 CCTCAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1451 CCTGGCCAAGG
EcoRI 785 GAATTC G^AATTC
2353 GAATTC
FauI 66 CCCGC CCCGC(4/6)
82 CCCGC
FbaI 985 TGATCA T^GATCA
2514 TGATCA
HaeII 97 GGCGCC [GA]GCGC^[CT]
2393 AGCGCC
HapII 72 CCGG C^CGG
95 CCGG
HincII 1814 GTTGAC GT[CT]^[GA]AC
2183 GTTGAC
HindII 1814 GTTGAC GT[CT]^[GA]AC
2183 GTTGAC
HpaII 72 CCGG C^CGG
95 CCGG
MfeI 215 CAATTG C^AATTG
1248 CAATTG
MluNI 524 TGGCCA TGG^CCA
1453 TGGCCA
MscI 524 TGGCCA TGG^CCA
1453 TGGCCA
MspI 72 CCGG C^CGG
95 CCGG
MunI 215 CAATTG C^AATTG
1248 CAATTG
NciI 71 CCCGG CC^[GC]GG
94 CCCGG
PstI 12 CTGCAG CTGCA^G
1353 CTGCAG
SapI 2775 GAAGAGC GCTCTTC(1/4)
956 GCTCTTC
Sau96I 64 GGCCC G^G[ACGT]CC
2573 GGCCC
SspI 1532 AATATT AAT^ATT
2418 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AseI AsnI BbvI
BfaI BpmI BsaI BsaJI BsgI
BsiYI BslI BsmAI BsmFI BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI DdeI
DpnII DraI EarI EcoRII EcoT14I
EcoT22I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MvnI MwoI NdeII NlaIII NlaIV
NsiI PshBI RsaI Sau3AI ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII