##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI ApaI
AscI Asp718I AspEI AviII AvrII
BbrPI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BssHII BssSI Bst1107I BstZ17I
CelII ClaI CpoI Eam1105I EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SgrAI SnaBI SpeI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AseI 868 ATTAAT AT^TAAT
AsnI 868 ATTAAT AT^TAAT
Asp700I 1020 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BciVI 811 GTATCC GTATCC(6/5)
BsaAI 1216 TACGTG [CT]AC^GT[GA]
BsiWI 1214 CGTACG C^GTACG
Bsp1407I 2457 TGTACA T^GTACA
BsrGI 2457 TGTACA T^GTACA
BstBI 1093 TTCGAA TT^CGAA
BstXI 2683 CCAGGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2468 TTTAAA TTT^AAA
DraIII 2360 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 831 CGGCCG C^GGCCG
EclXI 831 CGGCCG C^GGCCG
Eco52I 831 CGGCCG C^GGCCG
EcoRI 1912 GAATTC G^AATTC
NdeI 1791 CATATG CA^TATG
NspV 1093 TTCGAA TT^CGAA
PshBI 868 ATTAAT AT^TAAT
ScaI 1873 AGTACT AGT^ACT
SexAI 1844 ACCAGGT A^CC[AT]GGT
SfiI 907 GGCCCAATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1093 TTCGAA TT^CGAA
SspBI 2457 TGTACA T^GTACA
XmnI 1020 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 1912 GAATTC [GA]^AATT[CT]
2235 AAATTC
AcyI 1006 GGCGCC G[GA]^CG[CT]C
2062 GGCGCC
Alw44I 1189 GTGCAC G^TGCAC
1219 GTGCAC
AocI 964 CCTCAGG CC^T[ACGT]AGG
3134 CCTCAGG
Aor51HI 690 AGCGCT AGC^GCT
1022 AGCGCT
ApaLI 1189 GTGCAC G^TGCAC
1219 GTGCAC
ApoI 1912 GAATTC [GA]^AATT[CT]
2235 AAATTC
BbeI 1006 GGCGCC GGCGC^C
2062 GGCGCC
BcgI 2022 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1376 GCAACGACTTCG
BsaHI 1006 GGCGCC G[GA]^CG[CT]C
2062 GGCGCC
BsiEI 831 CGGCCG CG[GA][CT]^CG
1136 CGACCG
BsmBI 3015 CGTCTC CGTCTC(1/5)
1210 GAGACG
BsmI 3441 GAATGC GAATGC(1/-1)
1551 GCATTC
BsrFI 1138 ACCGGC [GA]^CCGG[CT]
2951 ACCGGC
BstEII 651 GGTCACC G^GT[ACGT]ACC
2155 GGTGACC
BstPI 651 GGTCACC G^GT[ACGT]ACC
2155 GGTGACC
Bsu36I 964 CCTCAGG CC^T[ACGT]AGG
3134 CCTCAGG
Cfr10I 1138 ACCGGC [GA]^CCGG[CT]
2951 ACCGGC
DrdI 2424 GACTGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2903 GACACTGATGTC
Eco47III 690 AGCGCT AGC^GCT
1022 AGCGCT
Eco81I 964 CCTCAGG CC^T[ACGT]AGG
3134 CCTCAGG
EcoNI 1413 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2648 CCTTGGCCAGG
EcoO65I 651 GGTCACC G^GT[ACGT]ACC
2155 GGTGACC
EcoT22I 1228 ATGCAT ATGCA^T
1709 ATGCAT
Hin1I 1006 GGCGCC G[GA]^CG[CT]C
2062 GGCGCC
HindIII 318 AAGCTT A^AGCTT
1642 AAGCTT
KasI 1006 GGCGCC G^GCGCC
2062 GGCGCC
MslI 654 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1507 CACTGCTGTG
NarI 1006 GGCGCC GG^CGCC
2062 GGCGCC
NsiI 1228 ATGCAT ATGCA^T
1709 ATGCAT
PflMI 102 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCATGGACTGG
SapI 1089 GCTCTTC GCTCTTC(1/4)
1341 GCTCTTC
SbfI 1489 CCTGCAGG CCTGCA^GG
3058 CCTGCAGG
SfoI 1006 GGCGCC GGC^GCC
2062 GGCGCC
SmlI 466 CTTGAG C^T[CT][GA]AG
1935 CTTGAG
SphI 431 GCATGC GCATG^C
1711 GCATGC
Sse8387I 1489 CCTGCAGG CCTGCA^GG
3058 CCTGCAGG
SspI 1657 AATATT AAT^ATT
1865 AATATT
TaqI 1094 TCGA T^CGA
1385 TCGA
TthHB8I 1094 TCGA T^CGA
1385 TCGA
Van91I 102 CCATGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
405 CCATGGACTGG
XbaI 1662 TCTAGA T^CTAGA
1908 TCTAGA
XcmI 379 CCAAGGCCTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
948 CCAAGGGGAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I XhoII XmaCI XmaI