##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI Asp718I
AspI AviII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BglI BlpI Bpu1102I BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EaeI
EagI EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaII KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 2540 GTAGAC GT^[AC][GT]AC
AhdI 2416 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3275 ATTAAT AT^TAAT
AsnI 3275 ATTAAT AT^TAAT
Asp700I 1405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2416 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 118 GAGCAC G[AT]GC[AT]^C
AvaI 2837 CTCGAG C^[CT]CG[GA]G
AvrII 421 CCTAGG C^CTAGG
BanII 2405 GGGCTC G[GA]GC[CT]^C
BciVI 1160 GGATAC GTATCC(6/5)
BglII 1953 AGATCT A^GATCT
BlnI 421 CCTAGG C^CTAGG
BsaBI 742 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 782 GTGCAG GTGCAG(16/14)
BsiHKAI 118 GAGCAC G[AT]GC[AT]^C
BsoBI 2837 CTCGAG C^[CT]CG[GA]G
BsrDI 2890 GCAATG GCAATG(2/0)
BssSI 2321 CACGAG CACGAG(-5/-1)
BstXI 2212 CCATTGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 2790 AGGACCT [GA]G^G[ACGT]CC[CT]
Eam1105I 2416 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2790 AGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 173 GACGC GACGC(5/10)
HpaI 2388 GTTAAC GTT^AAC
MamI 742 GATTCTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 2432 CACCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 785 CAGCTG C[AC]G^C[GT]G
NdeI 2198 CATATG CA^TATG
PaeR7I 2837 CTCGAG C^TCGAG
PflMI 2938 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2790 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 3275 ATTAAT AT^TAAT
PvuII 785 CAGCTG CAG^CTG
Van91I 2938 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1916 CCACTGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2837 CTCGAG C^TCGAG
XmnI 1405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 2470 CCGC CCGC(-3/-1)
1144 GCGG
AflII 161 CTTAAG C^TTAAG
3128 CTTAAG
AlwI 2146 GATCC GGATC(4/5)
2395 GATCC
AocI 7 CCTTAGG CC^T[ACGT]AGG
2993 CCTCAGG
BbsI 1384 GAAGAC GAAGAC(2/6)
1492 GAAGAC
BfrI 161 CTTAAG C^TTAAG
3128 CTTAAG
BmyI 118 GAGCAC G[AGT]GC[ACT]^C
2405 GGGCTC
BpuAI 1384 GAAGAC GAAGAC(2/6)
1492 GAAGAC
BsmI 2049 GAATGC GAATGC(1/-1)
2190 GCATTC
Bsp1286I 118 GAGCAC G[AGT]GC[ACT]^C
2405 GGGCTC
BspMI 2877 ACCTGC ACCTGC(4/8)
3326 GCAGGT
BstYI 1953 AGATCT [GA]^GATC[CT]
2145 AGATCC
Bsu36I 7 CCTTAGG CC^T[ACGT]AGG
2993 CCTCAGG
Eco81I 7 CCTTAGG CC^T[ACGT]AGG
2993 CCTCAGG
MflI 1953 AGATCT [GA]^GATC[CT]
2145 AGATCC
NspI 131 GCATGT [GA]CATG^[CT]
1398 ACATGC
SapI 1687 GAAGAGC GCTCTTC(1/4)
1921 GAAGAGC
SspI 656 AATATT AAT^ATT
3062 AATATT
SwaI 2660 ATTTAAAT ATTT^AAAT
3207 ATTTAAAT
TaqI 2159 TCGA T^CGA
2838 TCGA
TthHB8I 2159 TCGA T^CGA
2838 TCGA
XbaI 1086 TCTAGA T^CTAGA
1170 TCTAGA
XhoII 1953 AGATCT [GA]^GATC[CT]
2145 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AcsI AfaI AluI ApoI AvaII
BbvI BfaI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoNI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HindIII
HinfI HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI