##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccIII AclI AflII Aor51HI
AscI AseI AsnI Asp700I AspI
BbrPI BciVI BclI BfrI BglII
BsaBI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrDI BsrGI
BstBI ClaI CpoI DraI Eco47III
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SalI ScaI SexAI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3006 GGTACC G^GTACC
AccI 3167 GTATAC GT^[AC][GT]AC
AcsI 908 GAATTC [GA]^AATT[CT]
AflIII 683 ACATGT A^C[GA][CT]GT
AgeI 2647 ACCGGT A^CCGGT
AhdI 785 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 74 GTGCAC G^TGCAC
ApaLI 74 GTGCAC G^TGCAC
ApoI 908 GAATTC [GA]^AATT[CT]
Asp718I 3006 GGTACC G^GTACC
AspEI 785 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2544 TGCGCA TGC^GCA
AvrII 3786 CCTAGG C^CTAGG
BamHI 15 GGATCC G^GATCC
BbeI 2925 GGCGCC GGCGC^C
BcgI 3085 CGACGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3786 CCTAGG C^CTAGG
BsaAI 3170 TACGTG [CT]AC^GT[GA]
BsiEI 3682 CGGCCG CG[GA][CT]^CG
BspHI 2173 TCATGA T^CATGA
BspLU11I 683 ACATGT A^CATGT
BssHII 3664 GCGCGC G^CGCGC
Bst1107I 3167 GTATAC GTA^TAC
BstZ17I 3167 GTATAC GTA^TAC
DrdI 502 GACCACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3682 CGGCCG C^GGCCG
Eam1105I 785 GACGCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3682 CGGCCG C^GGCCG
Eco52I 3682 CGGCCG C^GGCCG
EcoRI 908 GAATTC G^AATTC
FspI 2544 TGCGCA TGC^GCA
KasI 2925 GGCGCC G^GCGCC
KpnI 3006 GGTACC GGTAC^C
KspI 239 CCGCGG CCGC^GG
NaeI 1195 GCCGGC GCC^GGC
NarI 2925 GGCGCC GG^CGCC
NgoMIV 1195 GCCGGC G^CCGGC
PinAI 2647 ACCGGT A^CCGGT
PshAI 1770 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2173 TCATGA T^CATGA
SacII 239 CCGCGG CCGC^GG
SbfI 1924 CCTGCAGG CCTGCA^GG
SfoI 2925 GGCGCC GGC^GCC
SmlI 2046 CTCAAG C^T[CT][GA]AG
Sse8387I 1924 CCTGCAGG CCTGCA^GG
XbaI 550 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AocI 1265 CCTGAGG CC^T[ACGT]AGG
1864 CCTGAGG
BbsI 1544 GTCTTC GAAGAC(2/6)
3134 GTCTTC
BbvCI 938 CCTCAGC CCTCAGC(-5/-2)
3263 GCTGAGG
BpuAI 1544 GTCTTC GAAGAC(2/6)
3134 GTCTTC
BsaWI 2443 ACCGGA [AT]^CCGG[AT]
2647 ACCGGT
BssSI 929 CACGAG CACGAG(-5/-1)
3521 CACGAG
BstYI 15 GGATCC [GA]^GATC[CT]
3549 AGATCC
Bsu36I 1265 CCTGAGG CC^T[ACGT]AGG
1864 CCTGAGG
DraIII 1089 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
2897 CACCGTGTG
Eco81I 1265 CCTGAGG CC^T[ACGT]AGG
1864 CCTGAGG
EcoNI 1939 CCTGGGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3613 CCTGGTGCAGG
MflI 15 GGATCC [GA]^GATC[CT]
3549 AGATCC
NcoI 439 CCATGG C^CATGG
2212 CCATGG
NspI 683 ACATGT [GA]CATG^[CT]
3558 ACATGC
SapI 2251 GCTCTTC GCTCTTC(1/4)
3562 GCTCTTC
Tsp509I 138 AATT ^AATT
909 AATT
XhoII 15 GGATCC [GA]^GATC[CT]
3549 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfiI SmaI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XmaCI XmaI