##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BcgI BclI BfrI BglII
BlnI BsaBI BsiWI BspDI BspHI
BspLU11I BsrDI BstBI ClaI CpoI
DraI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HpaI
KasI MamI MfeI MluI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII ScaI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI Tru9I Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1479 GGTACC G^GTACC
AccIII 831 TCCGGA T^CCGGA
AcsI 1940 AAATTC [GA]^AATT[CT]
Alw44I 3140 GTGCAC G^TGCAC
AocI 3447 CCTGAGG CC^T[ACGT]AGG
ApaLI 3140 GTGCAC G^TGCAC
ApoI 1940 AAATTC [GA]^AATT[CT]
Asp718I 1479 GGTACC G^GTACC
BamHI 2053 GGATCC G^GATCC
BbrPI 41 CACGTG CAC^GTG
BbvCI 3263 CCTCAGC CCTCAGC(-5/-2)
BsaI 790 GGTCTC GGTCTC(1/5)
BsaWI 831 TCCGGA [AT]^CCGG[AT]
BseAI 831 TCCGGA T^CCGGA
BsmBI 1574 CGTCTC CGTCTC(1/5)
Bsp1407I 840 TGTACA T^GTACA
BspEI 831 TCCGGA T^CCGGA
BsrGI 840 TGTACA T^GTACA
Bst1107I 508 GTATAC GTA^TAC
BstEII 760 GGTGACC G^GT[ACGT]ACC
BstPI 760 GGTGACC G^GT[ACGT]ACC
BstYI 2053 GGATCC [GA]^GATC[CT]
BstZ17I 508 GTATAC GTA^TAC
Bsu36I 3447 CCTGAGG CC^T[ACGT]AGG
DraIII 1493 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1430 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 896 CGGCCG C^GGCCG
EclXI 896 CGGCCG C^GGCCG
Eco52I 896 CGGCCG C^GGCCG
Eco81I 3447 CCTGAGG CC^T[ACGT]AGG
EcoO65I 760 GGTGACC G^GT[ACGT]ACC
HindIII 3506 AAGCTT A^AGCTT
KpnI 1479 GGTACC GGTAC^C
MflI 2053 GGATCC [GA]^GATC[CT]
MroI 831 TCCGGA T^CCGGA
MslI 658 CACGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PmaCI 41 CACGTG CAC^GTG
PmlI 41 CACGTG CAC^GTG
SalI 2171 GTCGAC G^TCGAC
SexAI 2550 ACCAGGT A^CC[AT]GGT
SfiI 2531 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2329 CCCGGG CCC^GGG
SmlI 2969 CTTGAG C^T[CT][GA]AG
SspBI 840 TGTACA T^GTACA
TaqI 2172 TCGA T^CGA
TfiI 3498 GATTC G^A[AT]TC
Tsp509I 1941 AATT ^AATT
TthHB8I 2172 TCGA T^CGA
XhoII 2053 GGATCC [GA]^GATC[CT]
XmaCI 2329 CCCGGG C^CCGGG
XmaI 2329 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 508 GTATAC GT^[AC][GT]AC
2171 GTCGAC
AcyI 1124 GGCGTC G[GA]^CG[CT]C
2468 GGCGTC
AlwI 2054 GATCC GGATC(4/5)
2053 GGATC
BalI 987 TGGCCA TGG^CCA
1338 TGGCCA
BanI 1479 GGTACC G^G[CT][GA]CC
2399 GGCACC
BbsI 1439 GTCTTC GAAGAC(2/6)
1545 GTCTTC
BpuAI 1439 GTCTTC GAAGAC(2/6)
1545 GTCTTC
BsaAI 41 CACGTG [CT]AC^GT[GA]
1493 CACGTA
BsaHI 1124 GGCGTC G[GA]^CG[CT]C
2468 GGCGTC
BsiEI 896 CGGCCG CG[GA][CT]^CG
2364 CGGTCG
BsmFI 1606 GGGAC GGGAC(10/14)
901 GTCCC
BsmI 325 GCATTC GAATGC(1/-1)
3354 GCATTC
BsrBI 1968 CCGCTC CCGCTC(-3/-3)
3239 CCGCTC
BsrFI 1147 GCCGGC [GA]^CCGG[CT]
2719 GCCGGC
BssHII 2929 GCGCGC G^CGCGC
3185 GCGCGC
BssSI 1796 CTCGTG CACGAG(-5/-1)
3278 CTCGTG
Cfr10I 1147 GCCGGC [GA]^CCGG[CT]
2719 GCCGGC
Hin1I 1124 GGCGTC G[GA]^CG[CT]C
2468 GGCGTC
HincII 1385 GTTGAC GT[CT]^[GA]AC
2171 GTCGAC
HindII 1385 GTTGAC GT[CT]^[GA]AC
2171 GTCGAC
KspI 74 CCGCGG CCGC^GG
2626 CCGCGG
MluNI 987 TGGCCA TGG^CCA
1338 TGGCCA
MscI 987 TGGCCA TGG^CCA
1338 TGGCCA
NaeI 1147 GCCGGC GCC^GGC
2719 GCCGGC
NgoMIV 1147 GCCGGC G^CCGGC
2719 GCCGGC
PleI 2703 GACTC GAGTC(4/5)
3015 GACTC
PpuMI 278 GGGTCCT [GA]G^G[AT]CC[CT]
732 AGGACCC
SacII 74 CCGCGG CCGC^GG
2626 CCGCGG
SphI 552 GCATGC GCATG^C
1525 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI Sse8387I StuI
StyI TseI Tsp45I TspRI Van91I
XcmI