##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlnI BsaI BseAI BsiWI BsmBI
BsmI Bsp1407I BspEI BsrFI BsrGI
Bst1107I BstBI BstXI BstZ17I Cfr10I
CpoI EagI Eam1105I EclXI Eco52I
EcoRI FseI FspI HindIII KpnI
KspI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Alw44I 288 GTGCAC G^TGCAC
AocI 3059 CCTGAGG CC^T[ACGT]AGG
Aor51HI 209 AGCGCT AGC^GCT
ApaI 2047 GGGCCC GGGCC^C
ApaLI 288 GTGCAC G^TGCAC
AseI 2744 ATTAAT AT^TAAT
AsnI 2744 ATTAAT AT^TAAT
Asp700I 1581 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 960 GGCGCC GGCGC^C
BbsI 2372 GTCTTC GAAGAC(2/6)
BclI 2681 TGATCA T^GATCA
BglI 448 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2372 GTCTTC GAAGAC(2/6)
BsaAI 1576 TACGTG [CT]AC^GT[GA]
BsaBI 1484 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 238 CGACCG CG[GA][CT]^CG
BspDI 2009 ATCGAT AT^CGAT
BspHI 1589 TCATGA T^CATGA
BssHII 457 GCGCGC G^CGCGC
BssSI 725 CACGAG CACGAG(-5/-1)
BstAPI 1456 GCACATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1757 GGTTACC G^GT[ACGT]ACC
BstPI 1757 GGTTACC G^GT[ACGT]ACC
BstYI 3554 AGATCC [GA]^GATC[CT]
Bsu36I 3059 CCTGAGG CC^T[ACGT]AGG
ClaI 2009 ATCGAT AT^CGAT
DraIII 1005 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 239 GACCGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1004 CCACGG C^C[GA][CT]GG
EarI 2130 GAAGAG CTCTTC(1/4)
Eco47III 209 AGCGCT AGC^GCT
Eco81I 3059 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1757 GGTTACC G^GT[ACGT]ACC
EcoRV 2159 GATATC GAT^ATC
EcoT14I 1081 CCTTGG C^C[AT][AT]GG
EcoT22I 2296 ATGCAT ATGCA^T
FbaI 2681 TGATCA T^GATCA
HincII 2596 GTTAAC GT[CT]^[GA]AC
HindII 2596 GTTAAC GT[CT]^[GA]AC
HpaI 2596 GTTAAC GTT^AAC
KasI 960 GGCGCC G^GCGCC
Ksp632I 2130 GAAGAG CTCTTC(1/4)
MamI 1484 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 3554 AGATCC [GA]^GATC[CT]
MslI 779 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 960 GGCGCC GG^CGCC
NsiI 2296 ATGCAT ATGCA^T
PaeR7I 1033 CTCGAG C^TCGAG
PpuMI 2409 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1016 GACGCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2744 ATTAAT AT^TAAT
RcaI 1589 TCATGA T^CATGA
SacI 2062 GAGCTC GAGCT^C
SfoI 960 GGCGCC GGC^GCC
SmlI 1033 CTCGAG C^T[CT][GA]AG
SphI 310 GCATGC GCATG^C
StyI 1081 CCTTGG C^C[AT][AT]GG
XcmI 1614 CCACCGTTAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1033 CTCGAG C^TCGAG
XhoII 3554 AGATCC [GA]^GATC[CT]
XmnI 1581 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwNI 345 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3168 CAGAAGCTG
AspHI 288 GTGCAC G[AT]GC[AT]^C
2062 GAGCTC
BalI 446 TGGCCA TGG^CCA
1696 TGGCCA
BanI 585 GGCACC G^G[CT][GA]CC
960 GGCGCC
BlpI 395 GCTCAGC GC^T[ACGT]AGC
644 GCTGAGC
Bpu1102I 395 GCTCAGC GC^T[ACGT]AGC
644 GCTGAGC
BsaWI 204 ACCGGA [AT]^CCGG[AT]
1899 TCCGGT
BsgI 354 GTGCAG GTGCAG(16/14)
1011 GTGCAG
BsiHKAI 288 GTGCAC G[AT]GC[AT]^C
2062 GAGCTC
BspLU11I 658 ACATGT A^CATGT
1513 ACATGT
BspMI 625 GCAGGT ACCTGC(4/8)
1227 GCAGGT
BsrBI 393 CCGCTC CCGCTC(-3/-3)
96 GAGCGG
BsrDI 2854 CATTGC GCAATG(2/0)
1271 GCAATG
CelII 395 GCTCAGC GC^T[ACGT]AGC
644 GCTGAGC
DraII 634 AGGCCCT [GA]G^G[ACGT]CC[CT]
2409 GGGTCCC
EaeI 446 TGGCCA [CT]^GGCC[GA]
1696 TGGCCA
EcoNI 350 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
939 CCTCCCGGAGG
EcoO109I 634 AGGCCCT [GA]G^G[ACGT]CC[CT]
2409 GGGTCCC
MluNI 446 TGGCCA TGG^CCA
1696 TGGCCA
MscI 446 TGGCCA TGG^CCA
1696 TGGCCA
PflMI 1267 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1688 CCAGCTCTTGG
PvuII 642 CAGCTG CAG^CTG
873 CAGCTG
SspI 3349 AATATT AAT^ATT
3580 AATATT
TfiI 1484 GATTC G^A[AT]TC
1896 GAATC
Van91I 1267 CCAGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1688 CCAGCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I