##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI Asp718I
AspEI AviII AvrII BbeI BbrPI
BcgI BfrI BglI BglII BlnI
BsaAI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BspDI BspEI BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO109I EcoRV
EcoT22I FauI FseI FspI HincII
HindII HpaI KasI KpnI KspI
MaeII MfeI MluI MroI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 2356 CGCG CG^CG
AflIII 1539 ACATGT A^C[GA][CT]GT
AspHI 1118 GAGCAC G[AT]GC[AT]^C
AspI 2475 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 950 GGATCC G^GATCC
BanII 1281 GAGCCC G[GA]GC[CT]^C
BbsI 698 GAAGAC GAAGAC(2/6)
BciVI 691 GGATAC GTATCC(6/5)
BclI 338 TGATCA T^GATCA
BpuAI 698 GAAGAC GAAGAC(2/6)
BsaBI 2891 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1271 GAGGAG GAGGAG(10/8)
BsiHKAI 1118 GAGCAC G[AT]GC[AT]^C
Bsp1407I 219 TGTACA T^GTACA
BspHI 2877 TCATGA T^CATGA
BspLU11I 1539 ACATGT A^CATGT
BsrDI 2062 GCAATG GCAATG(2/0)
BsrGI 219 TGTACA T^GTACA
BssSI 2391 CACGAG CACGAG(-5/-1)
BstEII 910 GGTTACC G^GT[ACGT]ACC
BstPI 910 GGTTACC G^GT[ACGT]ACC
BstUI 2356 CGCG CG^CG
DraIII 617 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1507 CCATGG C^C[GA][CT]GG
EcoNI 2711 CCTATATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 910 GGTTACC G^GT[ACGT]ACC
EcoRI 1220 GAATTC G^AATTC
FbaI 338 TGATCA T^GATCA
MamI 2891 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 930 CAGCTG C[AC]G^C[GT]G
MvnI 2356 CGCG CG^CG
NcoI 1507 CCATGG C^CATGG
NdeI 223 CATATG CA^TATG
PacI 2758 TTAATTAA TTAAT^TAA
PflFI 2475 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 701 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 927 CTGCAG CTGCA^G
PvuII 930 CAGCTG CAG^CTG
RcaI 2877 TCATGA T^CATGA
SexAI 1164 ACCAGGT A^CC[AT]GGT
SmaI 2408 CCCGGG CCC^GGG
SmlI 2376 CTCAAG C^T[CT][GA]AG
SphI 867 GCATGC GCATG^C
SspBI 219 TGTACA T^GTACA
TaqI 1333 TCGA T^CGA
Tth111I 2475 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1333 TCGA T^CGA
XmaCI 2408 CCCGGG C^CCGGG
XmaI 2408 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcyI 705 GGCGTC G[GA]^CG[CT]C
959 GGCGTC
Aor51HI 627 AGCGCT AGC^GCT
1028 AGCGCT
AseI 386 ATTAAT AT^TAAT
1746 ATTAAT
AsnI 386 ATTAAT AT^TAAT
1746 ATTAAT
Asp700I 547 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1804 GAATAAATTC
AvaI 872 CCCGAG C^[CT]CG[GA]G
2408 CCCGGG
BalI 2294 TGGCCA TGG^CCA
2905 TGGCCA
BanI 1051 GGCACC G^G[CT][GA]CC
1209 GGCACC
BbvCI 2384 GCTGAGG CCTCAGC(-5/-2)
2995 GCTGAGG
BcnI 2408 CCCGG CC^[GC]GG
2409 CCGGG
BlpI 1115 GCTGAGC GC^T[ACGT]AGC
2205 GCTGAGC
BmyI 1118 GAGCAC G[AGT]GC[ACT]^C
1281 GAGCCC
BpmI 2452 CTCCAG CTGGAG(16/14)
3062 CTCCAG
Bpu1102I 1115 GCTGAGC GC^T[ACGT]AGC
2205 GCTGAGC
BsaHI 705 GGCGTC G[GA]^CG[CT]C
959 GGCGTC
BsgI 2447 CTGCAC GTGCAG(16/14)
3057 CTGCAC
BsoBI 872 CCCGAG C^[CT]CG[GA]G
2408 CCCGGG
Bsp1286I 1118 GAGCAC G[AGT]GC[ACT]^C
1281 GAGCCC
BstYI 950 GGATCC [GA]^GATC[CT]
1347 AGATCC
CelII 1115 GCTGAGC GC^T[ACGT]AGC
2205 GCTGAGC
Eco47III 627 AGCGCT AGC^GCT
1028 AGCGCT
HaeII 627 AGCGCT [GA]GCGC^[CT]
1028 AGCGCT
Hin1I 705 GGCGTC G[GA]^CG[CT]C
959 GGCGTC
HindIII 108 AAGCTT A^AGCTT
1915 AAGCTT
MflI 950 GGATCC [GA]^GATC[CT]
1347 AGATCC
MluNI 2294 TGGCCA TGG^CCA
2905 TGGCCA
MscI 2294 TGGCCA TGG^CCA
2905 TGGCCA
NciI 2408 CCCGG CC^[GC]GG
2409 CCGGG
PflMI 710 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
890 CCAACCTCTGG
PshBI 386 ATTAAT AT^TAAT
1746 ATTAAT
Van91I 710 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
890 CCAACCTCTGG
XcmI 1059 CCAACTCAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1929 CCAATTTCCTGATGG
XhoII 950 GGATCC [GA]^GATC[CT]
1347 AGATCC
XmnI 547 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1804 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BfaI BsaJI
BsiYI BslI BsmAI BsmBI BsmFI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI