##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
BbrPI BfrI BglII BlpI Bpu1102I
BsaWI BseAI BsgI BsiEI BsiWI
BspDI BspEI BspLU11I BsrDI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI FspI KpnI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 114 CGCG CG^CG
AocI 1271 CCTGAGG CC^T[ACGT]AGG
Asp700I 537 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1688 CCTAGG C^CTAGG
BalI 1298 TGGCCA TGG^CCA
BamHI 176 GGATCC G^GATCC
BanI 1360 GGCGCC G^G[CT][GA]CC
BbeI 1360 GGCGCC GGCGC^C
BbsI 2595 GTCTTC GAAGAC(2/6)
BbvCI 1388 GCTGAGG CCTCAGC(-5/-2)
BciVI 1266 GGATAC GTATCC(6/5)
BlnI 1688 CCTAGG C^CTAGG
BpuAI 2595 GTCTTC GAAGAC(2/6)
BsaAI 958 TACGTG [CT]AC^GT[GA]
BsaBI 1013 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 615 GAGACC GGTCTC(1/5)
BsmI 541 GCATTC GAATGC(1/-1)
Bsp1407I 2454 TGTACA T^GTACA
BspHI 505 TCATGA T^CATGA
BspMI 2493 ACCTGC ACCTGC(4/8)
BsrBI 13 CCGCTC CCGCTC(-3/-3)
BsrGI 2454 TGTACA T^GTACA
BstUI 114 CGCG CG^CG
Bsu36I 1271 CCTGAGG CC^T[ACGT]AGG
Eco81I 1271 CCTGAGG CC^T[ACGT]AGG
HaeII 1360 GGCGCC [GA]GCGC^[CT]
HincII 2271 GTTAAC GT[CT]^[GA]AC
HindII 2271 GTTAAC GT[CT]^[GA]AC
HindIII 1577 AAGCTT A^AGCTT
HpaI 2271 GTTAAC GTT^AAC
KasI 1360 GGCGCC G^GCGCC
KspI 113 CCGCGG CCGC^GG
MamI 1013 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1298 TGGCCA TGG^CCA
MscI 1298 TGGCCA TGG^CCA
MvnI 114 CGCG CG^CG
NarI 1360 GGCGCC GG^CGCC
NcoI 811 CCATGG C^CATGG
NspI 2047 GCATGC [GA]CATG^[CT]
PaeR7I 300 CTCGAG C^TCGAG
PflMI 754 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1208 CAGCTG CAG^CTG
RcaI 505 TCATGA T^CATGA
SacII 113 CCGCGG CCGC^GG
SfcI 1365 CTGTAG C^T[GA][CT]AG
SfiI 2021 GGCCTCTTAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1360 GGCGCC GGC^GCC
SphI 2047 GCATGC GCATG^C
SspBI 2454 TGTACA T^GTACA
SspI 1632 AATATT AAT^ATT
StuI 916 AGGCCT AGG^CCT
Van91I 754 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1105 TCTAGA T^CTAGA
XhoI 300 CTCGAG C^TCGAG
XmnI 537 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 418 GTCTAC GT^[AC][GT]AC
2166 GTCTAC
AcyI 1360 GGCGCC G[GA]^CG[CT]C
1479 GACGCC
AlwNI 755 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2301 CAGGGCCTG
ApaI 66 GGGCCC GGGCC^C
1054 GGGCCC
AseI 2117 ATTAAT AT^TAAT
2581 ATTAAT
AsnI 2117 ATTAAT AT^TAAT
2581 ATTAAT
AspI 437 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
465 GACAAGGTC
AvaI 54 CTCGGG C^[CT]CG[GA]G
300 CTCGAG
BcgI 302 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1394 GCAGGAGAATCG
BclI 517 TGATCA T^GATCA
1703 TGATCA
BcnI 96 CCGGG CC^[GC]GG
275 CCGGG
BglI 909 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2022 GCCTCTTAGGC
BsaHI 1360 GGCGCC G[GA]^CG[CT]C
1479 GACGCC
BsoBI 54 CTCGGG C^[CT]CG[GA]G
300 CTCGAG
EaeI 634 TGGCCG [CT]^GGCC[GA]
1298 TGGCCA
EcoRI 152 GAATTC G^AATTC
509 GAATTC
FauI 112 CCCGC CCCGC(4/6)
2320 GCGGG
FbaI 517 TGATCA T^GATCA
1703 TGATCA
HgaI 1479 GACGC GACGC(5/10)
346 GCGTC
Hin1I 1360 GGCGCC G[GA]^CG[CT]C
1479 GACGCC
MaeII 62 ACGT A^CGT
959 ACGT
MslI 1019 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2366 CACCAAAATG
MspA1I 113 CCGCGG C[AC]G^C[GT]G
1208 CAGCTG
NciI 96 CCGGG CC^[GC]GG
275 CCGGG
PflFI 437 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
465 GACAAGGTC
PshBI 2117 ATTAAT AT^TAAT
2581 ATTAAT
Tsp45I 1075 GTGAC ^GT[GC]AC
2328 GTCAC
Tth111I 437 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
465 GACAAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XcmI XhoII