##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AgeI Aor51HI ApaI AscI Asp718I
AspI AviII BamHI BbrPI BbvCI
BglI BlpI Bpu1102I BseAI BsiEI
BsiWI BsmBI BspDI BspEI BsrBI
BssHII BssSI Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
ClaI CpoI DraII DraIII EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRV FauI FseI FspI
HgaI KpnI KspI MluI MroI
MspA1I MvnI NaeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I StuI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 821 GTCTAC GT^[AC][GT]AC
AcyI 1715 GGCGCC G[GA]^CG[CT]C
AflII 1742 CTTAAG C^TTAAG
AflIII 2560 ACATGT A^C[GA][CT]GT
AhdI 1632 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 319 GTGCAC G^TGCAC
AlwNI 1754 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 319 GTGCAC G^TGCAC
AseI 1232 ATTAAT AT^TAAT
AsnI 1232 ATTAAT AT^TAAT
AspEI 1632 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 319 GTGCAC G[AT]GC[AT]^C
AvaI 1242 CTCGGG C^[CT]CG[GA]G
AvrII 2236 CCTAGG C^CTAGG
BbeI 1715 GGCGCC GGCGC^C
BciVI 1780 GGATAC GTATCC(6/5)
BclI 2377 TGATCA T^GATCA
BcnI 2067 CCCGG CC^[GC]GG
BfrI 1742 CTTAAG C^TTAAG
BglII 3356 AGATCT A^GATCT
BlnI 2236 CCTAGG C^CTAGG
BsaAI 1872 CACGTA [CT]AC^GT[GA]
BsaBI 3351 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1715 GGCGCC G[GA]^CG[CT]C
BsaI 1156 GAGACC GGTCTC(1/5)
BsaWI 2654 ACCGGA [AT]^CCGG[AT]
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
BsoBI 1242 CTCGGG C^[CT]CG[GA]G
Bsp1407I 813 TGTACA T^GTACA
BspHI 2530 TCATGA T^CATGA
BspLU11I 2560 ACATGT A^CATGT
BspMI 224 GCAGGT ACCTGC(4/8)
BsrFI 1112 ACCGGC [GA]^CCGG[CT]
BsrGI 813 TGTACA T^GTACA
BstBI 2096 TTCGAA TT^CGAA
CfoI 1716 GCGC GCG^C
Cfr10I 1112 ACCGGC [GA]^CCGG[CT]
DrdI 1442 GACAGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1632 GACGAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2830 GAATTC G^AATTC
FbaI 2377 TGATCA T^GATCA
HaeII 1715 GGCGCC [GA]GCGC^[CT]
HhaI 1716 GCGC GCG^C
Hin1I 1715 GGCGCC G[GA]^CG[CT]C
HinP1I 1716 GCGC G^CGC
HpaI 2929 GTTAAC GTT^AAC
KasI 1715 GGCGCC G^GCGCC
MamI 3351 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2130 CAATTG C^AATTG
MunI 2130 CAATTG C^AATTG
NarI 1715 GGCGCC GG^CGCC
NciI 2067 CCCGG CC^[GC]GG
NdeI 1039 CATATG CA^TATG
NspV 2096 TTCGAA TT^CGAA
PshBI 1232 ATTAAT AT^TAAT
RcaI 2530 TCATGA T^CATGA
SfcI 504 CTACAG C^T[GA][CT]AG
SfoI 1715 GGCGCC GGC^GCC
SfuI 2096 TTCGAA TT^CGAA
SphI 1990 GCATGC GCATG^C
SspBI 813 TGTACA T^GTACA
XcmI 1076 CCAGAACCAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 1160 CCTGAGG CC^T[ACGT]AGG
2342 CCTGAGG
AvaII 979 GGACC G^G[AT]CC
3188 GGTCC
BalI 1373 TGGCCA TGG^CCA
2404 TGGCCA
BanI 1165 GGTGCC G^G[CT][GA]CC
1715 GGCGCC
BanII 2493 GGGCTC G[GA]GC[CT]^C
3274 GAGCCC
BbsI 3027 GAAGAC GAAGAC(2/6)
399 GTCTTC
BcgI 2056 CGAAATAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2753 CGAAGATGATGC
BpmI 2780 CTCCAG CTGGAG(16/14)
1944 CTGGAG
BpuAI 3027 GAAGAC GAAGAC(2/6)
399 GTCTTC
BstYI 1147 AGATCC [GA]^GATC[CT]
3356 AGATCT
Bsu36I 1160 CCTGAGG CC^T[ACGT]AGG
2342 CCTGAGG
EaeI 1373 TGGCCA [CT]^GGCC[GA]
2404 TGGCCA
Eco81I 1160 CCTGAGG CC^T[ACGT]AGG
2342 CCTGAGG
HincII 2820 GTTGAC GT[CT]^[GA]AC
2929 GTTAAC
HindII 2820 GTTGAC GT[CT]^[GA]AC
2929 GTTAAC
MflI 1147 AGATCC [GA]^GATC[CT]
3356 AGATCT
MluNI 1373 TGGCCA TGG^CCA
2404 TGGCCA
MscI 1373 TGGCCA TGG^CCA
2404 TGGCCA
PleI 1733 GACTC GAGTC(4/5)
1823 GAGTC
ScaI 854 AGTACT AGT^ACT
1387 AGTACT
XhoII 1147 AGATCC [GA]^GATC[CT]
3356 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BbvI BfaI BmyI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NcoI NdeII NlaIII NlaIV NsiI
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI