##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AgeI Alw44I ApaLI ApoI
AseI AsnI Asp700I Asp718I AvrII
BbrPI BcgI BciVI BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
DraI EcoRI EcoRV EcoT22I FseI
HincII HindII HpaI KpnI MamI
MluI MroI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SacI SalI ScaI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AflII 1771 CTTAAG C^TTAAG
AflIII 2883 ACGTGT A^C[GA][CT]GT
AhdI 3055 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 1014 GGCGCGCC GG^CGCGCC
AspEI 3055 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 2621 GAGCAC G[AT]GC[AT]^C
AspI 7 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2144 TGCGCA TGC^GCA
BamHI 1994 GGATCC G^GATCC
BbsI 1573 GTCTTC GAAGAC(2/6)
BbvCI 21 CCTCAGC CCTCAGC(-5/-2)
BclI 1565 TGATCA T^GATCA
BfrI 1771 CTTAAG C^TTAAG
BpuAI 1573 GTCTTC GAAGAC(2/6)
BsaI 2156 GAGACC GGTCTC(1/5)
BsiHKAI 2621 GAGCAC G[AT]GC[AT]^C
CpoI 1160 CGGTCCG CG^G[AT]CCG
DraIII 2575 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1726 GACCAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3055 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1565 TGATCA T^GATCA
FspI 2144 TGCGCA TGC^GCA
HindIII 823 AAGCTT A^AGCTT
MfeI 1874 CAATTG C^AATTG
MunI 1874 CAATTG C^AATTG
PflFI 7 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1113 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 1160 CGGTCCG CG^G[AT]CCG
SbfI 1407 CCTGCAGG CCTGCA^GG
SexAI 8 ACCAGGT A^CC[AT]GGT
Sse8387I 1407 CCTGCAGG CCTGCA^GG
TaqI 2102 TCGA T^CGA
TfiI 862 GAATC G^A[AT]TC
Tth111I 7 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2102 TCGA T^CGA
Van91I 1113 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 209 CCAGCTGACGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AocI 445 CCTGAGG CC^T[ACGT]AGG
1539 CCTCAGG
BalI 1382 TGGCCA TGG^CCA
3345 TGGCCA
BfaI 1492 CTAG C^TAG
1508 CTAG
BglII 1901 AGATCT A^GATCT
2797 AGATCT
BlpI 2340 GCTGAGC GC^T[ACGT]AGC
2618 GCTGAGC
Bpu1102I 2340 GCTGAGC GC^T[ACGT]AGC
2618 GCTGAGC
BsaAI 2302 TACGTG [CT]AC^GT[GA]
2882 TACGTG
BsrDI 2028 CATTGC GCAATG(2/0)
2897 CATTGC
BstAPI 2064 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2574 GCACCCAGTGC
BstEII 2918 GGTCACC G^GT[ACGT]ACC
3189 GGTTACC
BstPI 2918 GGTCACC G^GT[ACGT]ACC
3189 GGTTACC
Bsu36I 445 CCTGAGG CC^T[ACGT]AGG
1539 CCTCAGG
CelII 2340 GCTGAGC GC^T[ACGT]AGC
2618 GCTGAGC
Eco81I 445 CCTGAGG CC^T[ACGT]AGG
1539 CCTCAGG
EcoNI 748 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1026 CCTGCGGGAGG
EcoO65I 2918 GGTCACC G^GT[ACGT]ACC
3189 GGTTACC
KspI 1037 CCGCGG CCGC^GG
1157 CCGCGG
MaeI 1492 CTAG C^TAG
1508 CTAG
MluNI 1382 TGGCCA TGG^CCA
3345 TGGCCA
MscI 1382 TGGCCA TGG^CCA
3345 TGGCCA
MslI 651 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1579 CACCGAGATG
NaeI 1053 GCCGGC GCC^GGC
1365 GCCGGC
NgoMIV 1053 GCCGGC G^CCGGC
1365 GCCGGC
PstI 1408 CTGCAG CTGCA^G
1855 CTGCAG
SacII 1037 CCGCGG CCGC^GG
1157 CCGCGG
SapI 1312 GAAGAGC GCTCTTC(1/4)
1909 GAAGAGC
SfcI 1408 CTGCAG C^T[GA][CT]AG
1855 CTGCAG
SfiI 1043 GGCCCGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3337 GGCCAGCCTGGCC
SmaI 2589 CCCGGG CCC^GGG
2955 CCCGGG
XmaCI 2589 CCCGGG C^CCGGG
2955 CCCGGG
XmaI 2589 CCCGGG C^CCGGG
2955 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI ApaI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssHII
BssKI BssSI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco47III
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfoI SmlI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII