##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbsI BcgI BciVI BglI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI HinP1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 2734 GACGTC GACGT^C
AccI 2731 GTCGAC GT^[AC][GT]AC
AcyI 2734 GACGTC G[GA]^CG[CT]C
Asp700I 413 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 368 GGATCC G^GATCC
BbrPI 2543 CACGTG CAC^GTG
BglII 2429 AGATCT A^GATCT
BpmI 2763 CTGGAG CTGGAG(16/14)
BsaHI 2734 GACGTC G[GA]^CG[CT]C
BsmBI 1045 GAGACG CGTCTC(1/5)
BsmI 2615 GAATGC GAATGC(1/-1)
Bsp1407I 2081 TGTACA T^GTACA
BspHI 947 TCATGA T^CATGA
BspLU11I 276 ACATGT A^CATGT
BsrDI 1867 GCAATG GCAATG(2/0)
BsrGI 2081 TGTACA T^GTACA
BstEII 1850 GGTAACC G^GT[ACGT]ACC
BstPI 1850 GGTAACC G^GT[ACGT]ACC
DraIII 2065 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1725 CCATGG C^C[GA][CT]GG
EarI 1674 CTCTTC CTCTTC(1/4)
EcoNI 2061 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1850 GGTAACC G^GT[ACGT]ACC
FauI 1108 CCCGC CCCGC(4/6)
Hin1I 2734 GACGTC G[GA]^CG[CT]C
HincII 2731 GTCGAC GT[CT]^[GA]AC
HindII 2731 GTCGAC GT[CT]^[GA]AC
Ksp632I 1674 CTCTTC CTCTTC(1/4)
MfeI 2077 CAATTG C^AATTG
MunI 2077 CAATTG C^AATTG
NcoI 1725 CCATGG C^CATGG
NlaIV 368 GGATCC GG[ACGT]^[ACGT]CC
PmaCI 2543 CACGTG CAC^GTG
PmlI 2543 CACGTG CAC^GTG
PpuMI 430 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 2028 CTGCAG CTGCA^G
RcaI 947 TCATGA T^CATGA
SalI 2731 GTCGAC G^TCGAC
SexAI 2066 ACCAGGT A^CC[AT]GGT
SmaI 2320 CCCGGG CCC^GGG
SspBI 2081 TGTACA T^GTACA
XmaCI 2320 CCCGGG C^CCGGG
XmaI 2320 CCCGGG C^CCGGG
XmnI 413 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 922 CTTAAG C^TTAAG
2554 CTTAAG
AvaI 1147 CCCGAG C^[CT]CG[GA]G
2320 CCCGGG
AvaII 431 GGTCC G^G[AT]CC
2470 GGACC
BclI 677 TGATCA T^GATCA
2626 TGATCA
BfrI 922 CTTAAG C^TTAAG
2554 CTTAAG
BsaAI 349 CACGTA [CT]AC^GT[GA]
2543 CACGTG
BsgI 1314 CTGCAC GTGCAG(16/14)
1076 GTGCAG
BsoBI 1147 CCCGAG C^[CT]CG[GA]G
2320 CCCGGG
BstAPI 2493 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2671 GCAAAAAATGC
DraI 829 TTTAAA TTT^AAA
1583 TTTAAA
DraII 430 AGGTCCT [GA]G^G[ACGT]CC[CT]
2419 AGGCCCT
EcoO109I 430 AGGTCCT [GA]G^G[ACGT]CC[CT]
2419 AGGCCCT
FbaI 677 TGATCA T^GATCA
2626 TGATCA
HapII 2236 CCGG C^CGG
2321 CCGG
HpaII 2236 CCGG C^CGG
2321 CCGG
MslI 475 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1544 CACAGGCATG
MspI 2236 CCGG C^CGG
2321 CCGG
PflMI 2255 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2605 CCAGGATGTGG
PleI 1651 GACTC GAGTC(4/5)
2399 GACTC
SacI 2056 GAGCTC GAGCT^C
2188 GAGCTC
SphI 1168 GCATGC GCATG^C
2667 GCATGC
TaqI 2732 TCGA T^CGA
2738 TCGA
Tsp45I 2164 GTGAC ^GT[GC]AC
2548 GTGAC
TthHB8I 2732 TCGA T^CGA
2738 TCGA
Van91I 2255 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2605 CCAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
BanII BbvCI BbvI BcnI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
Eco81I EcoRII EcoT14I Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NciI NdeII NlaIII NspI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII