##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AspEI AspHI AspI AvaI
AviII AvrII BbeI BbvCI BcgI
BcnI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BseAI BsiHKAI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraIII Eam1105I Eco47III
Eco81I EcoNI EcoO65I EcoRI FseI
FspI Hin1I KasI KspI MamI
MfeI MluI MroI MslI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmeI PpuMI PshAI Psp1406I
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 1420 CGCG CG^CG
AflIII 1588 ACACGT A^C[GA][CT]GT
AgeI 526 ACCGGT A^CCGGT
ApaI 83 GGGCCC GGGCC^C
AseI 2414 ATTAAT AT^TAAT
AsnI 2414 ATTAAT AT^TAAT
AvaII 1706 GGTCC G^G[AT]CC
BbrPI 1259 CACGTG CAC^GTG
BciVI 861 GGATAC GTATCC(6/5)
BglI 76 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1821 CTGGAG CTGGAG(16/14)
BsaAI 1259 CACGTG [CT]AC^GT[GA]
BsaI 1110 GAGACC GGTCTC(1/5)
BsaWI 526 ACCGGT [AT]^CCGG[AT]
BseRI 946 GAGGAG GAGGAG(10/8)
BsgI 2873 CTGCAC GTGCAG(16/14)
BsmI 2577 GAATGC GAATGC(1/-1)
BspMI 506 ACCTGC ACCTGC(4/8)
BstUI 1420 CGCG CG^CG
DraI 600 TTTAAA TTT^AAA
EagI 67 CGGCCG C^GGCCG
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoRV 1090 GATATC GAT^ATC
EcoT22I 263 ATGCAT ATGCA^T
HaeII 2268 AGCGCC [GA]GCGC^[CT]
HgaI 110 GCGTC GACGC(5/10)
HpaI 322 GTTAAC GTT^AAC
MvnI 1420 CGCG CG^CG
NdeI 2556 CATATG CA^TATG
NsiI 263 ATGCAT ATGCA^T
PflMI 1653 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 526 ACCGGT A^CCGGT
PmaCI 1259 CACGTG CAC^GTG
PmlI 1259 CACGTG CAC^GTG
PshBI 2414 ATTAAT AT^TAAT
PstI 1007 CTGCAG CTGCA^G
PvuI 42 CGATCG CGAT^CG
SapI 2624 GAAGAGC GCTCTTC(1/4)
SgrAI 525 CACCGGTG C[GA]^CCGG[CT]G
SspI 887 AATATT AAT^ATT
StuI 1835 AGGCCT AGG^CCT
Van91I 1653 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 542 CCAGAGAAGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 568 GGTACC G^GTACC
1543 GGTACC
AflII 1447 CTTAAG C^TTAAG
2234 CTTAAG
Asp700I 238 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2110 GAAGAGGTTC
Asp718I 568 GGTACC G^GTACC
1543 GGTACC
BamHI 3 GGATCC G^GATCC
2786 GGATCC
BanI 568 GGTACC G^G[CT][GA]CC
1543 GGTACC
BclI 1490 TGATCA T^GATCA
2433 TGATCA
BfrI 1447 CTTAAG C^TTAAG
2234 CTTAAG
BsiEI 42 CGATCG CG[GA][CT]^CG
67 CGGCCG
BspHI 2337 TCATGA T^CATGA
2946 TCATGA
BsrFI 65 ACCGGC [GA]^CCGG[CT]
526 ACCGGT
Cac8I 72 GCACGC GC[ACGT]^[ACGT]GC
2300 GCCAGC
CfoI 1317 GCGC GCG^C
2269 GCGC
Cfr10I 65 ACCGGC [GA]^CCGG[CT]
526 ACCGGT
DrdI 120 GACCTTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1852 GACAGGAAGGTC
DsaI 953 CCATGG C^C[GA][CT]GG
1184 CCATGG
FauI 1418 CCCGC CCCGC(4/6)
2428 CCCGC
FbaI 1490 TGATCA T^GATCA
2433 TGATCA
HapII 66 CCGG C^CGG
527 CCGG
HhaI 1317 GCGC GCG^C
2269 GCGC
HinP1I 1317 GCGC G^CGC
2269 GCGC
HincII 322 GTTAAC GT[CT]^[GA]AC
636 GTCAAC
HindII 322 GTTAAC GT[CT]^[GA]AC
636 GTCAAC
HpaII 66 CCGG C^CGG
527 CCGG
KpnI 568 GGTACC GGTAC^C
1543 GGTACC
MaeII 1260 ACGT A^CGT
1590 ACGT
MspI 66 CCGG C^CGG
527 CCGG
NcoI 953 CCATGG C^CATGG
1184 CCATGG
NspI 513 GCATGT [GA]CATG^[CT]
2442 ACATGC
PvuII 1932 CAGCTG CAG^CTG
2745 CAGCTG
RcaI 2337 TCATGA T^CATGA
2946 TCATGA
SfcI 1007 CTGCAG C^T[GA][CT]AG
2742 CTACAG
XmnI 238 GAAGCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2110 GAAGAGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BalI BanII BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII