##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AccIII AclI AflII AhdI AscI
AseI AsnI AspEI AspI AvaI
AviII BbeI BcgI BciVI BfrI
BlpI Bpu1102I BsaBI BseAI BsiWI
BsoBI Bsp1407I BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
CpoI DraI DrdI Eam1105I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I RcaI RsrII SacII
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AatII 2957 GACGTC GACGT^C
Acc65I 176 GGTACC G^GTACC
AcyI 2957 GACGTC G[GA]^CG[CT]C
AgeI 206 ACCGGT A^CCGGT
Aor51HI 1270 AGCGCT AGC^GCT
ApaI 3062 GGGCCC GGGCC^C
Asp718I 176 GGTACC G^GTACC
AvrII 3058 CCTAGG C^CTAGG
BbvCI 1937 CCTCAGC CCTCAGC(-5/-2)
BclI 528 TGATCA T^GATCA
BglI 2328 GCCGGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 133 AGATCT A^GATCT
BlnI 3058 CCTAGG C^CTAGG
BsaHI 2957 GACGTC G[GA]^CG[CT]C
BsaI 1101 GGTCTC GGTCTC(1/5)
BspDI 1422 ATCGAT AT^CGAT
BssSI 768 CTCGTG CACGAG(-5/-1)
BstAPI 2967 GCATTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2315 GGTCACC G^GT[ACGT]ACC
BstPI 2315 GGTCACC G^GT[ACGT]ACC
ClaI 1422 ATCGAT AT^CGAT
DsaI 2575 CCACGG C^C[GA][CT]GG
EagI 3151 CGGCCG C^GGCCG
EclXI 3151 CGGCCG C^GGCCG
Eco47III 1270 AGCGCT AGC^GCT
Eco52I 3151 CGGCCG C^GGCCG
EcoO65I 2315 GGTCACC G^GT[ACGT]ACC
EcoRI 2767 GAATTC G^AATTC
FbaI 528 TGATCA T^GATCA
HgaI 810 GCGTC GACGC(5/10)
Hin1I 2957 GACGTC G[GA]^CG[CT]C
KpnI 176 GGTACC GGTAC^C
NaeI 1216 GCCGGC GCC^GGC
NgoMIV 1216 GCCGGC G^CCGGC
PinAI 206 ACCGGT A^CCGGT
SacI 2600 GAGCTC GAGCT^C
SalI 1535 GTCGAC G^TCGAC
SapI 214 GAAGAGC GCTCTTC(1/4)
SbfI 3495 CCTGCAGG CCTGCA^GG
Sse8387I 3495 CCTGCAGG CCTGCA^GG
SspI 3105 AATATT AAT^ATT
TfiI 2719 GAATC G^A[AT]TC
XcmI 2832 CCAGCATCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 624 GTCTAC GT^[AC][GT]AC
1535 GTCGAC
AccII 281 CGCG CG^CG
961 CGCG
AcsI 2767 GAATTC [GA]^AATT[CT]
3251 GAATTT
Alw44I 465 GTGCAC G^TGCAC
471 GTGCAC
ApaLI 465 GTGCAC G^TGCAC
471 GTGCAC
ApoI 2767 GAATTC [GA]^AATT[CT]
3251 GAATTT
Asp700I 2638 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3103 GAAATATTTC
BamHI 695 GGATCC G^GATCC
1550 GGATCC
BsmBI 811 CGTCTC CGTCTC(1/5)
2163 CGTCTC
BsmI 1740 GCATTC GAATGC(1/-1)
2976 GCATTC
BspLU11I 2583 ACATGT A^CATGT
2665 ACATGT
BsrDI 1116 CATTGC GCAATG(2/0)
3473 GCAATG
BstUI 281 CGCG CG^CG
961 CGCG
BstXI 2217 CCATGCGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2886 CCACCATTGTGG
EcoNI 164 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1567 CCTTCTTGAGG
HincII 1310 GTTGAC GT[CT]^[GA]AC
1535 GTCGAC
HindII 1310 GTTGAC GT[CT]^[GA]AC
1535 GTCGAC
MseI 399 TTAA T^TAA
1986 TTAA
MvnI 281 CGCG CG^CG
961 CGCG
PflMI 226 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCACAGGATGG
PpuMI 67 AGGTCCT [GA]G^G[AT]CC[CT]
536 GGGTCCT
PvuI 277 CGATCG CGAT^CG
1424 CGATCG
ScaI 450 AGTACT AGT^ACT
2988 AGTACT
SphI 312 GCATGC GCATG^C
2253 GCATGC
StuI 403 AGGCCT AGG^CCT
1029 AGGCCT
Tru9I 399 TTAA T^TAA
1986 TTAA
Tsp509I 2768 AATT ^AATT
3252 AATT
Van91I 226 CCATCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1371 CCACAGGATGG
XmnI 2638 GAATAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3103 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AocI AspHI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII