################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI Aor51HI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AvrII BanI BbeI BbrPI BbvCI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoT22I FseI HaeII Hin1I HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 2452 GTAGAC GT^[AC][GT]AC AclI 2704 AACGTT AA^CGTT AflIII 2205 ACATGT A^C[GA][CT]GT Alw44I 2469 GTGCAC G^TGCAC AocI 3324 CCTGAGG CC^T[ACGT]AGG ApaLI 2469 GTGCAC G^TGCAC AviII 3085 TGCGCA TGC^GCA BamHI 1568 GGATCC G^GATCC BbsI 1982 GAAGAC GAAGAC(2/6) BcgI 2732 GCAACACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1982 GAAGAC GAAGAC(2/6) BsmBI 2320 GAGACG CGTCTC(1/5) Bsp1407I 1963 TGTACA T^GTACA BspLU11I 2205 ACATGT A^CATGT BsrGI 1963 TGTACA T^GTACA BstBI 1041 TTCGAA TT^CGAA Bsu36I 3324 CCTGAGG CC^T[ACGT]AGG CfoI 3086 GCGC GCG^C DsaI 1851 CCATGG C^C[GA][CT]GG Eco81I 3324 CCTGAGG CC^T[ACGT]AGG FauI 124 GCGGG CCCGC(4/6) FspI 3085 TGCGCA TGC^GCA HhaI 3086 GCGC GCG^C HinP1I 3086 GCGC G^CGC HindIII 2387 AAGCTT A^AGCTT MspA1I 900 CAGCTG C[AC]G^C[GT]G NcoI 1851 CCATGG C^CATGG NdeI 2762 CATATG CA^TATG NspV 1041 TTCGAA TT^CGAA PflMI 47 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 2704 AACGTT AA^CGTT PstI 2189 CTGCAG CTGCA^G PvuII 900 CAGCTG CAG^CTG SacI 827 GAGCTC GAGCT^C SfcI 2189 CTGCAG C^T[GA][CT]AG SfuI 1041 TTCGAA TT^CGAA SspBI 1963 TGTACA T^GTACA StuI 1345 AGGCCT AGG^CCT Van91I 47 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2901 CCATCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 105 CCGC CCGC(-3/-1) 124 GCGG AspHI 827 GAGCTC G[AT]GC[AT]^C 2469 GTGCAC BclI 1500 TGATCA T^GATCA 2772 TGATCA BsgI 3488 CTGCAC GTGCAG(16/14) 384 GTGCAG BsiHKAI 827 GAGCTC G[AT]GC[AT]^C 2469 GTGCAC BsmAI 2320 GAGAC GTCTC(1/5) 2214 GTCTC BsmFI 1123 GGGAC GGGAC(10/14) 1510 GGGAC BspMI 808 ACCTGC ACCTGC(4/8) 1195 GCAGGT BstAPI 473 GCAGTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 896 GCAGCAGCTGC BstF5I 3477 CATCC GGATG(2/0) 397 GGATG BstXI 1845 CCAAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1920 CCATGAAACTGG BstYI 1320 AGATCC [GA]^GATC[CT] 1568 GGATCC EcoT14I 739 CCTTGG C^C[AT][AT]GG 1851 CCATGG FbaI 1500 TGATCA T^GATCA 2772 TGATCA FokI 3477 CATCC GGATG(9/13) 397 GGATG HapII 801 CCGG C^CGG 2045 CCGG HgaI 2322 GACGC GACGC(5/10) 1879 GCGTC HincII 866 GTTGAC GT[CT]^[GA]AC 2607 GTTGAC HindII 866 GTTGAC GT[CT]^[GA]AC 2607 GTTGAC HpaII 801 CCGG C^CGG 2045 CCGG MfeI 933 CAATTG C^AATTG 2550 CAATTG MflI 1320 AGATCC [GA]^GATC[CT] 1568 GGATCC MspI 801 CCGG C^CGG 2045 CCGG MunI 933 CAATTG C^AATTG 2550 CAATTG NspI 1354 GCATGT [GA]CATG^[CT] 2205 ACATGT StyI 739 CCTTGG C^C[AT][AT]GG 1851 CCATGG TfiI 1234 GATTC G^A[AT]TC 1468 GAATC XhoII 1320 AGATCC [GA]^GATC[CT] 1568 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 77 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AvaII BalI BanII BbvI BciVI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BspHI BsrDI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraI DraII EaeI EarI EcoO109I EcoRII EcoRV Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MvaI MwoI NdeII NlaIII NlaIV PleI PpuMI PshBI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SmlI SspI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I