##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AvrII BanI BbeI BbrPI
BbvCI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoT22I FseI
HaeII Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 2452 GTAGAC GT^[AC][GT]AC
AclI 2704 AACGTT AA^CGTT
AflIII 2205 ACATGT A^C[GA][CT]GT
Alw44I 2469 GTGCAC G^TGCAC
AocI 3324 CCTGAGG CC^T[ACGT]AGG
ApaLI 2469 GTGCAC G^TGCAC
AviII 3085 TGCGCA TGC^GCA
BamHI 1568 GGATCC G^GATCC
BbsI 1982 GAAGAC GAAGAC(2/6)
BcgI 2732 GCAACACTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1982 GAAGAC GAAGAC(2/6)
BsmBI 2320 GAGACG CGTCTC(1/5)
Bsp1407I 1963 TGTACA T^GTACA
BspLU11I 2205 ACATGT A^CATGT
BsrGI 1963 TGTACA T^GTACA
BstBI 1041 TTCGAA TT^CGAA
Bsu36I 3324 CCTGAGG CC^T[ACGT]AGG
CfoI 3086 GCGC GCG^C
DsaI 1851 CCATGG C^C[GA][CT]GG
Eco81I 3324 CCTGAGG CC^T[ACGT]AGG
FauI 124 GCGGG CCCGC(4/6)
FspI 3085 TGCGCA TGC^GCA
HhaI 3086 GCGC GCG^C
HinP1I 3086 GCGC G^CGC
HindIII 2387 AAGCTT A^AGCTT
MspA1I 900 CAGCTG C[AC]G^C[GT]G
NcoI 1851 CCATGG C^CATGG
NdeI 2762 CATATG CA^TATG
NspV 1041 TTCGAA TT^CGAA
PflMI 47 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2704 AACGTT AA^CGTT
PstI 2189 CTGCAG CTGCA^G
PvuII 900 CAGCTG CAG^CTG
SacI 827 GAGCTC GAGCT^C
SfcI 2189 CTGCAG C^T[GA][CT]AG
SfuI 1041 TTCGAA TT^CGAA
SspBI 1963 TGTACA T^GTACA
StuI 1345 AGGCCT AGG^CCT
Van91I 47 CCACACATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2901 CCATCATGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 105 CCGC CCGC(-3/-1)
124 GCGG
AspHI 827 GAGCTC G[AT]GC[AT]^C
2469 GTGCAC
BclI 1500 TGATCA T^GATCA
2772 TGATCA
BsgI 3488 CTGCAC GTGCAG(16/14)
384 GTGCAG
BsiHKAI 827 GAGCTC G[AT]GC[AT]^C
2469 GTGCAC
BsmAI 2320 GAGAC GTCTC(1/5)
2214 GTCTC
BsmFI 1123 GGGAC GGGAC(10/14)
1510 GGGAC
BspMI 808 ACCTGC ACCTGC(4/8)
1195 GCAGGT
BstAPI 473 GCAGTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
896 GCAGCAGCTGC
BstF5I 3477 CATCC GGATG(2/0)
397 GGATG
BstXI 1845 CCAAATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1920 CCATGAAACTGG
BstYI 1320 AGATCC [GA]^GATC[CT]
1568 GGATCC
EcoT14I 739 CCTTGG C^C[AT][AT]GG
1851 CCATGG
FbaI 1500 TGATCA T^GATCA
2772 TGATCA
FokI 3477 CATCC GGATG(9/13)
397 GGATG
HapII 801 CCGG C^CGG
2045 CCGG
HgaI 2322 GACGC GACGC(5/10)
1879 GCGTC
HincII 866 GTTGAC GT[CT]^[GA]AC
2607 GTTGAC
HindII 866 GTTGAC GT[CT]^[GA]AC
2607 GTTGAC
HpaII 801 CCGG C^CGG
2045 CCGG
MfeI 933 CAATTG C^AATTG
2550 CAATTG
MflI 1320 AGATCC [GA]^GATC[CT]
1568 GGATCC
MspI 801 CCGG C^CGG
2045 CCGG
MunI 933 CAATTG C^AATTG
2550 CAATTG
NspI 1354 GCATGT [GA]CATG^[CT]
2205 ACATGT
StyI 739 CCTTGG C^C[AT][AT]GG
1851 CCATGG
TfiI 1234 GATTC G^A[AT]TC
1468 GAATC
XhoII 1320 AGATCC [GA]^GATC[CT]
1568 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AfaI AluI AlwI AlwNI
ApoI AseI AsnI AvaII BalI
BanII BbvI BciVI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BspHI BsrDI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoRV Fnu4HI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
PshBI RcaI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SmlI SspI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I