##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI BbrPI BcgI
BciVI BclI BfrI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI Eam1105I
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SfiI SfuI SgrAI
SnaBI SpeI StuI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1928 GTCGAC GT^[AC][GT]AC
AcsI 2371 AAATTT [GA]^AATT[CT]
AflIII 2517 ACGTGT A^C[GA][CT]GT
Alw44I 1913 GTGCAC G^TGCAC
ApaLI 1913 GTGCAC G^TGCAC
ApoI 2371 AAATTT [GA]^AATT[CT]
Asp700I 2041 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 665 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2239 TGCGCA TGC^GCA
AvrII 1117 CCTAGG C^CTAGG
BamHI 3045 GGATCC G^GATCC
BbeI 1703 GGCGCC GGCGC^C
BbsI 3237 GAAGAC GAAGAC(2/6)
BlnI 1117 CCTAGG C^CTAGG
BpuAI 3237 GAAGAC GAAGAC(2/6)
BsaWI 270 ACCGGA [AT]^CCGG[AT]
BspHI 672 TCATGA T^CATGA
BsrBI 10 CCGCTC CCGCTC(-3/-3)
BssSI 1620 CTCGTG CACGAG(-5/-1)
BstAPI 2759 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
FspI 2239 TGCGCA TGC^GCA
HaeII 1703 GGCGCC [GA]GCGC^[CT]
HincII 1928 GTCGAC GT[CT]^[GA]AC
HindII 1928 GTCGAC GT[CT]^[GA]AC
HindIII 1886 AAGCTT A^AGCTT
KasI 1703 GGCGCC G^GCGCC
NaeI 2637 GCCGGC GCC^GGC
NarI 1703 GGCGCC GG^CGCC
NgoMIV 2637 GCCGGC G^CCGGC
PflFI 665 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 723 GACTCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 672 TCATGA T^CATGA
SacI 695 GAGCTC GAGCT^C
SalI 1928 GTCGAC G^TCGAC
SapI 42 GAAGAGC GCTCTTC(1/4)
SbfI 1771 CCTGCAGG CCTGCA^GG
ScaI 408 AGTACT AGT^ACT
SexAI 438 ACCTGGT A^CC[AT]GGT
SfoI 1703 GGCGCC GGC^GCC
Sse8387I 1771 CCTGCAGG CCTGCA^GG
SspI 3429 AATATT AAT^ATT
Tth111I 665 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 2041 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 2349 CGCG CG^CG
2611 CGCG
AlwNI 304 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
887 CAGGAGCTG
BglII 1942 AGATCT A^GATCT
2007 AGATCT
BsmI 103 GAATGC GAATGC(1/-1)
2032 GAATGC
Bsp1407I 2329 TGTACA T^GTACA
3532 TGTACA
BsrDI 1453 CATTGC GCAATG(2/0)
582 GCAATG
BsrGI 2329 TGTACA T^GTACA
3532 TGTACA
BstUI 2349 CGCG CG^CG
2611 CGCG
DraIII 437 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2776 CACGGTGTG
DrdI 24 GACGCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2591 GACGAGCAGGTC
EagI 1171 CGGCCG C^GGCCG
2657 CGGCCG
EclXI 1171 CGGCCG C^GGCCG
2657 CGGCCG
Eco52I 1171 CGGCCG C^GGCCG
2657 CGGCCG
MaeII 2517 ACGT A^CGT
3413 ACGT
MseI 1968 TTAA T^TAA
3597 TTAA
MvnI 2349 CGCG CG^CG
2611 CGCG
NcoI 627 CCATGG C^CATGG
1083 CCATGG
SspBI 2329 TGTACA T^GTACA
3532 TGTACA
Tru9I 1968 TTAA T^TAA
3597 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI