##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI Asp700I Asp718I AspEI BcgI
BciVI BfrI BsaBI BsiWI BsmBI
BsmI BspDI BsrDI BssHII Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoRI
EcoRV EcoT22I FseI KpnI KspI
MamI MfeI MluI MunI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI Sse8387I SspI SwaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 2492 CCTAAGG CC^T[ACGT]AGG
AseI 2395 ATTAAT AT^TAAT
AsnI 2395 ATTAAT AT^TAAT
AspI 302 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1230 TGCGCA TGC^GCA
BamHI 1704 GGATCC G^GATCC
BbrPI 527 CACGTG CAC^GTG
BclI 1068 TGATCA T^GATCA
BglII 773 AGATCT A^GATCT
BsaAI 527 CACGTG [CT]AC^GT[GA]
BsaI 1938 GAGACC GGTCTC(1/5)
BsiEI 277 CGGCCG CG[GA][CT]^CG
Bsp1407I 2747 TGTACA T^GTACA
BspHI 1368 TCATGA T^CATGA
BspLU11I 455 ACATGT A^CATGT
BsrGI 2747 TGTACA T^GTACA
BssSI 1789 CACGAG CACGAG(-5/-1)
Bsu36I 2492 CCTAAGG CC^T[ACGT]AGG
EagI 277 CGGCCG C^GGCCG
EclXI 277 CGGCCG C^GGCCG
Eco52I 277 CGGCCG C^GGCCG
Eco81I 2492 CCTAAGG CC^T[ACGT]AGG
EcoNI 3321 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1068 TGATCA T^GATCA
FspI 1230 TGCGCA TGC^GCA
HpaI 989 GTTAAC GTT^AAC
NaeI 279 GCCGGC GCC^GGC
NdeI 2814 CATATG CA^TATG
NgoMIV 279 GCCGGC G^CCGGC
PflFI 302 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 527 CACGTG CAC^GTG
PmlI 527 CACGTG CAC^GTG
PshBI 2395 ATTAAT AT^TAAT
RcaI 1368 TCATGA T^CATGA
SgrAI 1237 CACCGGCG C[GA]^CCGG[CT]G
SmaI 3268 CCCGGG CCC^GGG
SspBI 2747 TGTACA T^GTACA
TfiI 1015 GAATC G^A[AT]TC
Tth111I 302 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1885 TCTAGA T^CTAGA
XmaCI 3268 CCCGGG C^CCGGG
XmaI 3268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 455 ACATGT A^C[GA][CT]GT
885 ACGTGT
ApaI 1260 GGGCCC GGGCC^C
1600 GGGCCC
AvrII 2354 CCTAGG C^CTAGG
2827 CCTAGG
BalI 819 TGGCCA TGG^CCA
2367 TGGCCA
BlnI 2354 CCTAGG C^CTAGG
2827 CCTAGG
BlpI 850 GCTCAGC GC^T[ACGT]AGC
2723 GCTGAGC
Bpu1102I 850 GCTCAGC GC^T[ACGT]AGC
2723 GCTGAGC
BstAPI 196 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
244 GCACTCCCTGC
BstEII 1993 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
BstPI 1993 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
CelII 850 GCTCAGC GC^T[ACGT]AGC
2723 GCTGAGC
EcoO65I 1993 GGTCACC G^GT[ACGT]ACC
2293 GGTCACC
HincII 989 GTTAAC GT[CT]^[GA]AC
1376 GTCAAC
HindII 989 GTTAAC GT[CT]^[GA]AC
1376 GTCAAC
HindIII 368 AAGCTT A^AGCTT
1796 AAGCTT
MluNI 819 TGGCCA TGG^CCA
2367 TGGCCA
MscI 819 TGGCCA TGG^CCA
2367 TGGCCA
PaeR7I 427 CTCGAG C^TCGAG
1139 CTCGAG
SmlI 427 CTCGAG C^T[CT][GA]AG
1139 CTCGAG
Tsp509I 816 AATT ^AATT
2921 AATT
XhoI 427 CTCGAG C^TCGAG
1139 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BsaWI BseAI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MroI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI StyI
TaqI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XhoII