##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbeI
BbsI BcgI BfrI BlpI Bpu1102I
BpuAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI BstBI CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoRV FseI HindIII
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 3418 CGCG CG^CG
AccIII 827 TCCGGA T^CCGGA
AcyI 3465 GGCGTC G[GA]^CG[CT]C
AfaI 3575 GTAC GT^AC
AgeI 943 ACCGGT A^CCGGT
Aor51HI 871 AGCGCT AGC^GCT
Asp700I 102 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2240 TGCGCA TGC^GCA
AvrII 2441 CCTAGG C^CTAGG
BbrPI 2998 CACGTG CAC^GTG
BciVI 3560 GTATCC GTATCC(6/5)
BglII 1898 AGATCT A^GATCT
BlnI 2441 CCTAGG C^CTAGG
BsaAI 2998 CACGTG [CT]AC^GT[GA]
BsaBI 2795 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3465 GGCGTC G[GA]^CG[CT]C
BsaI 3188 GGTCTC GGTCTC(1/5)
BseAI 827 TCCGGA T^CCGGA
BsmBI 3590 GAGACG CGTCTC(1/5)
BspEI 827 TCCGGA T^CCGGA
BsrDI 3361 GCAATG GCAATG(2/0)
Bst1107I 428 GTATAC GTA^TAC
BstUI 3418 CGCG CG^CG
BstZ17I 428 GTATAC GTA^TAC
Eco47III 871 AGCGCT AGC^GCT
EcoRI 808 GAATTC G^AATTC
EcoT22I 3381 ATGCAT ATGCA^T
FspI 2240 TGCGCA TGC^GCA
HaeII 871 AGCGCT [GA]GCGC^[CT]
Hin1I 3465 GGCGTC G[GA]^CG[CT]C
MamI 2795 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 827 TCCGGA T^CCGGA
MvnI 3418 CGCG CG^CG
NdeI 2409 CATATG CA^TATG
NsiI 3381 ATGCAT ATGCA^T
PinAI 943 ACCGGT A^CCGGT
PmaCI 2998 CACGTG CAC^GTG
PmlI 2998 CACGTG CAC^GTG
RsaI 3575 GTAC GT^AC
SalI 1562 GTCGAC G^TCGAC
SapI 1126 GCTCTTC GCTCTTC(1/4)
ScaI 3574 AGTACT AGT^ACT
SmlI 1960 CTTGAG C^T[CT][GA]AG
SphI 1099 GCATGC GCATG^C
XmnI 102 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 428 GTATAC GT^[AC][GT]AC
1562 GTCGAC
AcsI 106 AAATTC [GA]^AATT[CT]
808 GAATTC
AflIII 2997 ACACGT A^C[GA][CT]GT
2999 ACGTGT
ApoI 106 AAATTC [GA]^AATT[CT]
808 GAATTC
AspHI 563 GAGCAC G[AT]GC[AT]^C
2514 GTGCTC
AvaI 169 CCCGGG C^[CT]CG[GA]G
2022 CCCGGG
BalI 249 TGGCCA TGG^CCA
1452 TGGCCA
BbvCI 351 CCTCAGC CCTCAGC(-5/-2)
867 CCTCAGC
BclI 1648 TGATCA T^GATCA
2447 TGATCA
BglI 1445 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1635 GCCCAGATGGC
BsiHKAI 563 GAGCAC G[AT]GC[AT]^C
2514 GTGCTC
BsoBI 169 CCCGGG C^[CT]CG[GA]G
2022 CCCGGG
BsrBI 234 CCGCTC CCGCTC(-3/-3)
449 CCGCTC
BsrFI 943 ACCGGT [GA]^CCGG[CT]
1314 ACCGGC
BstAPI 1135 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1249 GCAGCCCATGC
Cfr10I 943 ACCGGT [GA]^CCGG[CT]
1314 ACCGGC
EcoNI 2333 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2395 CCTGGGCTAGG
FbaI 1648 TGATCA T^GATCA
2447 TGATCA
HgaI 457 GACGC GACGC(5/10)
3466 GCGTC
HpaI 1025 GTTAAC GTT^AAC
3411 GTTAAC
MluNI 249 TGGCCA TGG^CCA
1452 TGGCCA
MscI 249 TGGCCA TGG^CCA
1452 TGGCCA
NspI 217 ACATGC [GA]CATG^[CT]
1099 GCATGC
SexAI 2552 ACCAGGT A^CC[AT]GGT
3148 ACCTGGT
SmaI 169 CCCGGG CCC^GGG
2022 CCCGGG
StuI 1680 AGGCCT AGG^CCT
2013 AGGCCT
XmaCI 169 CCCGGG C^CCGGG
2022 CCCGGG
XmaI 169 CCCGGG C^CCGGG
2022 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
ApaI AvaII BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII