##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AclI AflII AgeI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BbeI BbrPI BclI BfrI BlnI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrFI BsrGI BssHII Bst1107I BstZ17I
Cfr10I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1312 GACGTC GACGT^C
AccI 121 GTCTAC GT^[AC][GT]AC
AccIII 759 TCCGGA T^CCGGA
AcyI 1312 GACGTC G[GA]^CG[CT]C
AflIII 236 ACATGT A^C[GA][CT]GT
AhdI 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1528 CCTCAGG CC^T[ACGT]AGG
Asp700I 3153 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 2392 GGATAC GTATCC(6/5)
BcnI 1737 CCGGG CC^[GC]GG
BlpI 2531 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2531 GCTGAGC GC^T[ACGT]AGC
BsaBI 2393 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1312 GACGTC G[GA]^CG[CT]C
BseAI 759 TCCGGA T^CCGGA
BspEI 759 TCCGGA T^CCGGA
BspHI 2937 TCATGA T^CATGA
BspLU11I 236 ACATGT A^CATGT
BssSI 584 CACGAG CACGAG(-5/-1)
BstBI 598 TTCGAA TT^CGAA
BstEII 2683 GGTGACC G^GT[ACGT]ACC
BstPI 2683 GGTGACC G^GT[ACGT]ACC
BstXI 2332 CCACCGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1528 CCTCAGG CC^T[ACGT]AGG
CelII 2531 GCTGAGC GC^T[ACGT]AGC
DraIII 2557 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1528 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2683 GGTGACC G^GT[ACGT]ACC
EcoT22I 504 ATGCAT ATGCA^T
Hin1I 1312 GACGTC G[GA]^CG[CT]C
MamI 2393 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 759 TCCGGA T^CCGGA
NciI 1737 CCGGG CC^[GC]GG
NsiI 504 ATGCAT ATGCA^T
NspV 598 TTCGAA TT^CGAA
PaeR7I 1556 CTCGAG C^TCGAG
PshAI 2460 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2937 TCATGA T^CATGA
SacI 2534 GAGCTC GAGCT^C
ScaI 795 AGTACT AGT^ACT
SfuI 598 TTCGAA TT^CGAA
SmlI 1556 CTCGAG C^T[CT][GA]AG
XhoI 1556 CTCGAG C^TCGAG
XmnI 3153 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 1078 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2548 CAGGACCTG
AspHI 907 GTGCTC G[AT]GC[AT]^C
2534 GAGCTC
BanI 2480 GGTGCC G^G[CT][GA]CC
2570 GGCACC
BanII 1981 GGGCTC G[GA]GC[CT]^C
2534 GAGCTC
BbvCI 534 CCTCAGC CCTCAGC(-5/-2)
2167 CCTCAGC
BcgI 600 CGAAGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2571 GCACCAGCATCG
BglII 1336 AGATCT A^GATCT
1679 AGATCT
BsaAI 1404 TACGTG [CT]AC^GT[GA]
1764 TACGTG
BsaI 744 GAGACC GGTCTC(1/5)
2327 GAGACC
BsaWI 759 TCCGGA [AT]^CCGG[AT]
2614 ACCGGA
BsiHKAI 907 GTGCTC G[AT]GC[AT]^C
2534 GAGCTC
BsmI 938 GAATGC GAATGC(1/-1)
2853 GAATGC
BspMI 2552 ACCTGC ACCTGC(4/8)
1091 GCAGGT
CfoI 842 GCGC GCG^C
2204 GCGC
DraI 3168 TTTAAA TTT^AAA
3240 TTTAAA
DrdI 604 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1413 GACTCCAGGGTC
DsaI 308 CCATGG C^C[GA][CT]GG
912 CCATGG
HhaI 842 GCGC GCG^C
2204 GCGC
HinP1I 842 GCGC G^CGC
2204 GCGC
NcoI 308 CCATGG C^CATGG
912 CCATGG
NspI 236 ACATGT [GA]CATG^[CT]
1719 GCATGT
PflMI 343 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1251 CCAGATCGTGG
PpuMI 523 GGGACCC [GA]G^G[AT]CC[CT]
2549 AGGACCT
SapI 2618 GAAGAGC GCTCTTC(1/4)
2808 GCTCTTC
SpeI 1884 ACTAGT A^CTAGT
2760 ACTAGT
StuI 422 AGGCCT AGG^CCT
1348 AGGCCT
Van91I 343 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1251 CCAGATCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BalI
BamHI BbsI BbvI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII