################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AclI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BbrPI BclI BfrI BlnI BsiEI BsiWI BsmBI Bsp1407I BspDI BsrFI BsrGI BssHII Bst1107I BstZ17I Cfr10I ClaI CpoI EagI EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MspA1I MunI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 1312 GACGTC GACGT^C AccI 121 GTCTAC GT^[AC][GT]AC AccIII 759 TCCGGA T^CCGGA AcyI 1312 GACGTC G[GA]^CG[CT]C AflIII 236 ACATGT A^C[GA][CT]GT AhdI 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1528 CCTCAGG CC^T[ACGT]AGG Asp700I 3153 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BciVI 2392 GGATAC GTATCC(6/5) BcnI 1737 CCGGG CC^[GC]GG BlpI 2531 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2531 GCTGAGC GC^T[ACGT]AGC BsaBI 2393 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1312 GACGTC G[GA]^CG[CT]C BseAI 759 TCCGGA T^CCGGA BspEI 759 TCCGGA T^CCGGA BspHI 2937 TCATGA T^CATGA BspLU11I 236 ACATGT A^CATGT BssSI 584 CACGAG CACGAG(-5/-1) BstBI 598 TTCGAA TT^CGAA BstEII 2683 GGTGACC G^GT[ACGT]ACC BstPI 2683 GGTGACC G^GT[ACGT]ACC BstXI 2332 CCACCGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1528 CCTCAGG CC^T[ACGT]AGG CelII 2531 GCTGAGC GC^T[ACGT]AGC DraIII 2557 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1312 GACGTCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1528 CCTCAGG CC^T[ACGT]AGG EcoO65I 2683 GGTGACC G^GT[ACGT]ACC EcoT22I 504 ATGCAT ATGCA^T Hin1I 1312 GACGTC G[GA]^CG[CT]C MamI 2393 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 759 TCCGGA T^CCGGA NciI 1737 CCGGG CC^[GC]GG NsiI 504 ATGCAT ATGCA^T NspV 598 TTCGAA TT^CGAA PaeR7I 1556 CTCGAG C^TCGAG PshAI 2460 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2937 TCATGA T^CATGA SacI 2534 GAGCTC GAGCT^C ScaI 795 AGTACT AGT^ACT SfuI 598 TTCGAA TT^CGAA SmlI 1556 CTCGAG C^T[CT][GA]AG XhoI 1556 CTCGAG C^TCGAG XmnI 3153 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 1078 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2548 CAGGACCTG AspHI 907 GTGCTC G[AT]GC[AT]^C 2534 GAGCTC BanI 2480 GGTGCC G^G[CT][GA]CC 2570 GGCACC BanII 1981 GGGCTC G[GA]GC[CT]^C 2534 GAGCTC BbvCI 534 CCTCAGC CCTCAGC(-5/-2) 2167 CCTCAGC BcgI 600 CGAAGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2571 GCACCAGCATCG BglII 1336 AGATCT A^GATCT 1679 AGATCT BsaAI 1404 TACGTG [CT]AC^GT[GA] 1764 TACGTG BsaI 744 GAGACC GGTCTC(1/5) 2327 GAGACC BsaWI 759 TCCGGA [AT]^CCGG[AT] 2614 ACCGGA BsiHKAI 907 GTGCTC G[AT]GC[AT]^C 2534 GAGCTC BsmI 938 GAATGC GAATGC(1/-1) 2853 GAATGC BspMI 2552 ACCTGC ACCTGC(4/8) 1091 GCAGGT CfoI 842 GCGC GCG^C 2204 GCGC DraI 3168 TTTAAA TTT^AAA 3240 TTTAAA DrdI 604 GACTGTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1413 GACTCCAGGGTC DsaI 308 CCATGG C^C[GA][CT]GG 912 CCATGG HhaI 842 GCGC GCG^C 2204 GCGC HinP1I 842 GCGC G^CGC 2204 GCGC NcoI 308 CCATGG C^CATGG 912 CCATGG NspI 236 ACATGT [GA]CATG^[CT] 1719 GCATGT PflMI 343 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1251 CCAGATCGTGG PpuMI 523 GGGACCC [GA]G^G[AT]CC[CT] 2549 AGGACCT SapI 2618 GAAGAGC GCTCTTC(1/4) 2808 GCTCTTC SpeI 1884 ACTAGT A^CTAGT 2760 ACTAGT StuI 422 AGGCCT AGG^CCT 1348 AGGCCT Van91I 343 CCAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1251 CCAGATCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BalI BamHI BbsI BbvI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XcmI XhoII