##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsoBI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstBI
BstEII BstPI BstUI CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MluI MroI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacI
SacII SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AflIII 530 ACATGT A^C[GA][CT]GT
AlwI 1178 GATCC GGATC(4/5)
AvaII 1593 GGACC G^G[AT]CC
BanII 1599 GGGCTC G[GA]GC[CT]^C
BbsI 514 GTCTTC GAAGAC(2/6)
BbvCI 961 CCTCAGC CCTCAGC(-5/-2)
BcnI 1357 CCGGG CC^[GC]GG
BmyI 1599 GGGCTC G[AGT]GC[ACT]^C
BpmI 897 CTCCAG CTGGAG(16/14)
BpuAI 514 GTCTTC GAAGAC(2/6)
BsaAI 1779 TACGTA [CT]AC^GT[GA]
BsaBI 777 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1381 GAGGAG GAGGAG(10/8)
BsiYI 961 CCTCAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 961 CCTCAGCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 1966 GAGAC GTCTC(1/5)
BsmI 3002 GAATGC GAATGC(1/-1)
Bsp1286I 1599 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1702 TGTACA T^GTACA
BspHI 1630 TCATGA T^CATGA
BspLU11I 530 ACATGT A^CATGT
BsrGI 1702 TGTACA T^GTACA
Bst1107I 1512 GTATAC GTA^TAC
BstAPI 1021 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1574 CCAGATGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1177 AGATCC [GA]^GATC[CT]
BstZ17I 1512 GTATAC GTA^TAC
DraII 226 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1501 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 226 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 2718 GATATC GAT^ATC
HapII 1357 CCGG C^CGG
HgaI 1024 GACGC GACGC(5/10)
HindIII 1977 AAGCTT A^AGCTT
HpaII 1357 CCGG C^CGG
HphI 2656 TCACC GGTGA(8/7)
MamI 777 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 823 CAATTG C^AATTG
MflI 1177 AGATCC [GA]^GATC[CT]
MspI 1357 CCGG C^CGG
MunI 823 CAATTG C^AATTG
NciI 1357 CCGGG CC^[GC]GG
NspI 530 ACATGT [GA]CATG^[CT]
RcaI 1630 TCATGA T^CATGA
SalI 2582 GTCGAC G^TCGAC
ScaI 893 AGTACT AGT^ACT
SnaBI 1779 TACGTA TAC^GTA
SspBI 1702 TGTACA T^GTACA
StuI 2214 AGGCCT AGG^CCT
TaqI 2583 TCGA T^CGA
TthHB8I 2583 TCGA T^CGA
XbaI 2495 TCTAGA T^CTAGA
XhoII 1177 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflII 1970 CTTAAG C^TTAAG
1980 CTTAAG
AocI 578 CCTTAGG CC^T[ACGT]AGG
702 CCTGAGG
AseI 2025 ATTAAT AT^TAAT
2159 ATTAAT
AsnI 2025 ATTAAT AT^TAAT
2159 ATTAAT
BalI 1131 TGGCCA TGG^CCA
2988 TGGCCA
BfrI 1970 CTTAAG C^TTAAG
1980 CTTAAG
BsgI 1250 CTGCAC GTGCAG(16/14)
1140 GTGCAG
Bsu36I 578 CCTTAGG CC^T[ACGT]AGG
702 CCTGAGG
Cac8I 1952 GCCTGC GC[ACGT]^[ACGT]GC
1990 GCTTGC
Cfr13I 227 GGCCC G^G[ACGT]CC
1593 GGACC
EaeI 1131 TGGCCA [CT]^GGCC[GA]
2988 TGGCCA
EarI 842 CTCTTC CTCTTC(1/4)
1963 GAAGAG
Eco81I 578 CCTTAGG CC^T[ACGT]AGG
702 CCTGAGG
EcoT22I 500 ATGCAT ATGCA^T
2395 ATGCAT
HincII 405 GTCAAC GT[CT]^[GA]AC
2582 GTCGAC
HindII 405 GTCAAC GT[CT]^[GA]AC
2582 GTCGAC
Ksp632I 842 CTCTTC CTCTTC(1/4)
1963 GAAGAG
MluNI 1131 TGGCCA TGG^CCA
2988 TGGCCA
MscI 1131 TGGCCA TGG^CCA
2988 TGGCCA
MspA1I 78 CAGCTG C[AC]G^C[GT]G
2542 CAGCTG
NsiI 500 ATGCAT ATGCA^T
2395 ATGCAT
PshBI 2025 ATTAAT AT^TAAT
2159 ATTAAT
PvuII 78 CAGCTG CAG^CTG
2542 CAGCTG
Sau96I 227 GGCCC G^G[ACGT]CC
1593 GGACC
Tsp45I 1549 GTCAC ^GT[GC]AC
1846 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwNI
ApoI Asp700I BbvI BfaI BsaJI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI DdeI DpnII DraI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MvaI
MwoI NdeI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp509I TspRI
XmnI