################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccI AflII AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BfrI BlnI BsaAI BsaBI BsiWI BspHI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FspI HincII HindII HpaI KpnI KspI MamI MfeI MunI NdeI NheI NotI NruI NsiI NspV PflFI PmaCI PmeI PmlI PshBI RcaI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccIII 3113 TCCGGA T^CCGGA AclI 2323 AACGTT AA^CGTT AcsI 1770 AAATTT [GA]^AATT[CT] AgeI 2447 ACCGGT A^CCGGT Alw44I 2496 GTGCAC G^TGCAC Aor51HI 2673 AGCGCT AGC^GCT ApaLI 2496 GTGCAC G^TGCAC ApoI 1770 AAATTT [GA]^AATT[CT] BclI 599 TGATCA T^GATCA BglII 458 AGATCT A^GATCT BseAI 3113 TCCGGA T^CCGGA BsmI 1908 GAATGC GAATGC(1/-1) Bsp1407I 140 TGTACA T^GTACA BspDI 2036 ATCGAT AT^CGAT BspEI 3113 TCCGGA T^CCGGA BsrDI 495 CATTGC GCAATG(2/0) BsrGI 140 TGTACA T^GTACA BssHII 926 GCGCGC G^CGCGC BstYI 458 AGATCT [GA]^GATC[CT] ClaI 2036 ATCGAT AT^CGAT DraI 1801 TTTAAA TTT^AAA DrdI 2911 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 764 CGGCCG C^GGCCG EarI 1487 CTCTTC CTCTTC(1/4) EclXI 764 CGGCCG C^GGCCG Eco47III 2673 AGCGCT AGC^GCT Eco52I 764 CGGCCG C^GGCCG FbaI 599 TGATCA T^GATCA FseI 1248 GGCCGGCC GGCCGG^CC Ksp632I 1487 CTCTTC CTCTTC(1/4) MflI 458 AGATCT [GA]^GATC[CT] MluI 2492 ACGCGT A^CGCGT MroI 3113 TCCGGA T^CCGGA PacI 2717 TTAATTAA TTAAT^TAA PinAI 2447 ACCGGT A^CCGGT Psp1406I 2323 AACGTT AA^CGTT PvuI 3042 CGATCG CGAT^CG SacI 151 GAGCTC GAGCT^C SbfI 3189 CCTGCAGG CCTGCA^GG SexAI 1751 ACCTGGT A^CC[AT]GGT SphI 1813 GCATGC GCATG^C Sse8387I 3189 CCTGCAGG CCTGCA^GG SspBI 140 TGTACA T^GTACA SspI 2075 AATATT AAT^ATT XbaI 2328 TCTAGA T^CTAGA XcmI 684 CCAGCAGGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 458 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 528 CCTGAGG CC^T[ACGT]AGG 3100 CCTCAGG AspHI 151 GAGCTC G[AT]GC[AT]^C 2496 GTGCAC BcgI 489 CGACCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3036 GCAGGCCGATCG BglI 83 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 214 GCCCAGGCGGC BlpI 1220 GCTGAGC GC^T[ACGT]AGC 2808 GCTGAGC BpmI 1332 CTGGAG CTGGAG(16/14) 2607 CTGGAG Bpu1102I 1220 GCTGAGC GC^T[ACGT]AGC 2808 GCTGAGC BsaI 919 GGTCTC GGTCTC(1/5) 3212 GGTCTC BsaWI 2447 ACCGGT [AT]^CCGG[AT] 3113 TCCGGA BsgI 420 CTGCAC GTGCAG(16/14) 2474 GTGCAG BsiHKAI 151 GAGCTC G[AT]GC[AT]^C 2496 GTGCAC BspLU11I 821 ACATGT A^CATGT 2500 ACATGT BspMI 1828 ACCTGC ACCTGC(4/8) 198 GCAGGT BsrI 2318 ACTGG ACTGG(1/-1) 3242 ACTGG BssSI 907 CTCGTG CACGAG(-5/-1) 1450 CTCGTG Bsu36I 528 CCTGAGG CC^T[ACGT]AGG 3100 CCTCAGG CelII 1220 GCTGAGC GC^T[ACGT]AGC 2808 GCTGAGC DraIII 1116 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 2585 CACACAGTG Eco81I 528 CCTGAGG CC^T[ACGT]AGG 3100 CCTCAGG EcoNI 528 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2873 CCTGTGTCAGG HindIII 1661 AAGCTT A^AGCTT 1779 AAGCTT NaeI 1249 GCCGGC GCC^GGC 1866 GCCGGC NgoMIV 1249 GCCGGC G^CCGGC 1866 GCCGGC PaeR7I 3 CTCGAG C^TCGAG 454 CTCGAG XhoI 3 CTCGAG C^TCGAG 454 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI ApaI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BmyI BpuAI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I