##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbrPI BbvCI BciVI BfrI BlnI
BsaAI BsaBI BsiWI BspHI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FspI HincII HindII HpaI
KpnI KspI MamI MfeI MunI
NdeI NheI NotI NruI NsiI
NspV PflFI PmaCI PmeI PmlI
PshBI RcaI RsrII SacII SalI
SapI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 3113 TCCGGA T^CCGGA
AclI 2323 AACGTT AA^CGTT
AcsI 1770 AAATTT [GA]^AATT[CT]
AgeI 2447 ACCGGT A^CCGGT
Alw44I 2496 GTGCAC G^TGCAC
Aor51HI 2673 AGCGCT AGC^GCT
ApaLI 2496 GTGCAC G^TGCAC
ApoI 1770 AAATTT [GA]^AATT[CT]
BclI 599 TGATCA T^GATCA
BglII 458 AGATCT A^GATCT
BseAI 3113 TCCGGA T^CCGGA
BsmI 1908 GAATGC GAATGC(1/-1)
Bsp1407I 140 TGTACA T^GTACA
BspDI 2036 ATCGAT AT^CGAT
BspEI 3113 TCCGGA T^CCGGA
BsrDI 495 CATTGC GCAATG(2/0)
BsrGI 140 TGTACA T^GTACA
BssHII 926 GCGCGC G^CGCGC
BstYI 458 AGATCT [GA]^GATC[CT]
ClaI 2036 ATCGAT AT^CGAT
DraI 1801 TTTAAA TTT^AAA
DrdI 2911 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 764 CGGCCG C^GGCCG
EarI 1487 CTCTTC CTCTTC(1/4)
EclXI 764 CGGCCG C^GGCCG
Eco47III 2673 AGCGCT AGC^GCT
Eco52I 764 CGGCCG C^GGCCG
FbaI 599 TGATCA T^GATCA
FseI 1248 GGCCGGCC GGCCGG^CC
Ksp632I 1487 CTCTTC CTCTTC(1/4)
MflI 458 AGATCT [GA]^GATC[CT]
MluI 2492 ACGCGT A^CGCGT
MroI 3113 TCCGGA T^CCGGA
PacI 2717 TTAATTAA TTAAT^TAA
PinAI 2447 ACCGGT A^CCGGT
Psp1406I 2323 AACGTT AA^CGTT
PvuI 3042 CGATCG CGAT^CG
SacI 151 GAGCTC GAGCT^C
SbfI 3189 CCTGCAGG CCTGCA^GG
SexAI 1751 ACCTGGT A^CC[AT]GGT
SphI 1813 GCATGC GCATG^C
Sse8387I 3189 CCTGCAGG CCTGCA^GG
SspBI 140 TGTACA T^GTACA
SspI 2075 AATATT AAT^ATT
XbaI 2328 TCTAGA T^CTAGA
XcmI 684 CCAGCAGGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 458 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 528 CCTGAGG CC^T[ACGT]AGG
3100 CCTCAGG
AspHI 151 GAGCTC G[AT]GC[AT]^C
2496 GTGCAC
BcgI 489 CGACCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3036 GCAGGCCGATCG
BglI 83 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
214 GCCCAGGCGGC
BlpI 1220 GCTGAGC GC^T[ACGT]AGC
2808 GCTGAGC
BpmI 1332 CTGGAG CTGGAG(16/14)
2607 CTGGAG
Bpu1102I 1220 GCTGAGC GC^T[ACGT]AGC
2808 GCTGAGC
BsaI 919 GGTCTC GGTCTC(1/5)
3212 GGTCTC
BsaWI 2447 ACCGGT [AT]^CCGG[AT]
3113 TCCGGA
BsgI 420 CTGCAC GTGCAG(16/14)
2474 GTGCAG
BsiHKAI 151 GAGCTC G[AT]GC[AT]^C
2496 GTGCAC
BspLU11I 821 ACATGT A^CATGT
2500 ACATGT
BspMI 1828 ACCTGC ACCTGC(4/8)
198 GCAGGT
BsrI 2318 ACTGG ACTGG(1/-1)
3242 ACTGG
BssSI 907 CTCGTG CACGAG(-5/-1)
1450 CTCGTG
Bsu36I 528 CCTGAGG CC^T[ACGT]AGG
3100 CCTCAGG
CelII 1220 GCTGAGC GC^T[ACGT]AGC
2808 GCTGAGC
DraIII 1116 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
2585 CACACAGTG
Eco81I 528 CCTGAGG CC^T[ACGT]AGG
3100 CCTCAGG
EcoNI 528 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2873 CCTGTGTCAGG
HindIII 1661 AAGCTT A^AGCTT
1779 AAGCTT
NaeI 1249 GCCGGC GCC^GGC
1866 GCCGGC
NgoMIV 1249 GCCGGC G^CCGGC
1866 GCCGGC
PaeR7I 3 CTCGAG C^TCGAG
454 CTCGAG
XhoI 3 CTCGAG C^TCGAG
454 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcnI BfaI BmyI
BpuAI BsaHI BsaJI BseRI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshAI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I