##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AspEI
AviII AvrII BamHI BbsI BciVI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaWI BseAI BsiWI
BspDI BspEI BspHI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I ClaI
CpoI DraIII Eam1105I Eco47III EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HindIII HpaI KspI MfeI MluI
MroI MslI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PaeR7I PinAI PmeI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 397 GACGTC GACGT^C
Acc65I 2092 GGTACC G^GTACC
AccI 814 GTAGAC GT^[AC][GT]AC
AccII 120 CGCG CG^CG
AlwNI 1289 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1522 CCTGAGG CC^T[ACGT]AGG
ApaI 129 GGGCCC GGGCC^C
Asp700I 1866 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2092 GGTACC G^GTACC
AspHI 405 GAGCAC G[AT]GC[AT]^C
AspI 6 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 67 CCCGAG C^[CT]CG[GA]G
BalI 244 TGGCCA TGG^CCA
BanII 129 GGGCCC G[GA]GC[CT]^C
BbvCI 459 CCTCAGC CCTCAGC(-5/-2)
BclI 2003 TGATCA T^GATCA
BglI 539 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 707 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 57 CGGCCG CG[GA][CT]^CG
BsiHKAI 405 GAGCAC G[AT]GC[AT]^C
BsmI 1623 GAATGC GAATGC(1/-1)
BsoBI 67 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1145 TGTACA T^GTACA
BspLU11I 1148 ACATGT A^CATGT
BspMI 699 GCAGGT ACCTGC(4/8)
BsrBI 167 GAGCGG CCGCTC(-3/-3)
BsrGI 1145 TGTACA T^GTACA
BstAPI 365 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 120 CGCG CG^CG
BstYI 706 GGATCT [GA]^GATC[CT]
Bsu36I 1522 CCTGAGG CC^T[ACGT]AGG
DrdI 2647 GACTTTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1265 CCGTGG C^C[GA][CT]GG
EagI 57 CGGCCG C^GGCCG
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
Eco81I 1522 CCTGAGG CC^T[ACGT]AGG
EcoT14I 2445 CCAAGG C^C[AT][AT]GG
FbaI 2003 TGATCA T^GATCA
HincII 2644 GTTGAC GT[CT]^[GA]AC
HindII 2644 GTTGAC GT[CT]^[GA]AC
KpnI 2092 GGTACC GGTAC^C
MamI 707 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 706 GGATCT [GA]^GATC[CT]
MluNI 244 TGGCCA TGG^CCA
MscI 244 TGGCCA TGG^CCA
MvnI 120 CGCG CG^CG
PacI 1879 TTAATTAA TTAAT^TAA
PflFI 6 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 639 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2571 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 2014 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1916 AGTACT AGT^ACT
SmlI 1387 CTCAAG C^T[CT][GA]AG
SspBI 1145 TGTACA T^GTACA
StyI 2445 CCAAGG C^C[AT][AT]GG
Tth111I 6 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 639 CCATTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 706 GGATCT [GA]^GATC[CT]
XmnI 1866 GAATCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AseI 1882 ATTAAT AT^TAAT
2486 ATTAAT
AsnI 1882 ATTAAT AT^TAAT
2486 ATTAAT
BbrPI 1141 CACGTG CAC^GTG
1248 CACGTG
BcgI 1215 CGACGTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1617 CGAGGAGAATGC
BsaAI 1141 CACGTG [CT]AC^GT[GA]
1248 CACGTG
BsaI 2100 GGTCTC GGTCTC(1/5)
2674 GGTCTC
BsgI 1206 CTGCAC GTGCAG(16/14)
141 GTGCAG
BsmBI 52 CGTCTC CGTCTC(1/5)
4 GAGACG
BsmFI 553 GGGAC GGGAC(10/14)
2599 GGGAC
BsrDI 317 GCAATG GCAATG(2/0)
548 GCAATG
DraI 435 TTTAAA TTT^AAA
1798 TTTAAA
EarI 740 GAAGAG CTCTTC(1/4)
1305 GAAGAG
EcoRI 1678 GAATTC G^AATTC
2898 GAATTC
HgaI 6 GACGC GACGC(5/10)
1128 GACGC
Ksp632I 740 GAAGAG CTCTTC(1/4)
1305 GAAGAG
MspA1I 541 CAGCTG C[AC]G^C[GT]G
2141 CAGCTG
NspI 784 GCATGT [GA]CATG^[CT]
1148 ACATGT
PmaCI 1141 CACGTG CAC^GTG
1248 CACGTG
PmlI 1141 CACGTG CAC^GTG
1248 CACGTG
PshBI 1882 ATTAAT AT^TAAT
2486 ATTAAT
PstI 1331 CTGCAG CTGCA^G
1816 CTGCAG
PvuII 541 CAGCTG CAG^CTG
2141 CAGCTG
XcmI 540 CCAGCTGGGCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2133 CCATTATTCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI ApoI AvaII BanI
BbeI BbvI BcnI BfaI BmyI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I