##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccI AccII AclI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I AspEI
BbrPI BcgI BciVI BfrI BglII
BsaAI BsaBI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT22I HincII HindII HpaI KspI
MamI MfeI MluI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
ScaI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1761 GGTACC G^GTACC
AcyI 1749 GGCGCC G[GA]^CG[CT]C
Asp718I 1761 GGTACC G^GTACC
AspI 488 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2717 TGCGCA TGC^GCA
AvrII 5 CCTAGG C^CTAGG
BalI 1619 TGGCCA TGG^CCA
BbeI 1749 GGCGCC GGCGC^C
BlnI 5 CCTAGG C^CTAGG
BsaHI 1749 GGCGCC G[GA]^CG[CT]C
BsaI 172 GAGACC GGTCTC(1/5)
BsmBI 3314 GAGACG CGTCTC(1/5)
DraIII 432 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 2860 GAATTC G^AATTC
FseI 9 GGCCGGCC GGCCGG^CC
FspI 2717 TGCGCA TGC^GCA
HgaI 3316 GACGC GACGC(5/10)
Hin1I 1749 GGCGCC G[GA]^CG[CT]C
KasI 1749 GGCGCC G^GCGCC
KpnI 1761 GGTACC GGTAC^C
MluNI 1619 TGGCCA TGG^CCA
MscI 1619 TGGCCA TGG^CCA
NaeI 10 GCCGGC GCC^GGC
NarI 1749 GGCGCC GG^CGCC
NgoMIV 10 GCCGGC G^CCGGC
PflFI 488 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SbfI 2016 CCTGCAGG CCTGCA^GG
SexAI 433 ACCTGGT A^CC[AT]GGT
SfiI 2014 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1749 GGCGCC GGC^GCC
Sse8387I 2016 CCTGCAGG CCTGCA^GG
Tth111I 488 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 2685 GAATTT [GA]^AATT[CT]
2860 GAATTC
AocI 3018 CCTGAGG CC^T[ACGT]AGG
3141 CCTGAGG
Aor51HI 1051 AGCGCT AGC^GCT
3523 AGCGCT
ApoI 2685 GAATTT [GA]^AATT[CT]
2860 GAATTC
BamHI 519 GGATCC G^GATCC
2370 GGATCC
BlpI 690 GCTCAGC GC^T[ACGT]AGC
2291 GCTCAGC
Bpu1102I 690 GCTCAGC GC^T[ACGT]AGC
2291 GCTCAGC
BspHI 557 TCATGA T^CATGA
1531 TCATGA
BsrBI 1924 CCGCTC CCGCTC(-3/-3)
1790 GAGCGG
BssSI 1104 CACGAG CACGAG(-5/-1)
2875 CTCGTG
Bsu36I 3018 CCTGAGG CC^T[ACGT]AGG
3141 CCTGAGG
CelII 690 GCTCAGC GC^T[ACGT]AGC
2291 GCTCAGC
DrdI 59 GACTTTCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1606 GACCCCCTGGTC
Eco47III 1051 AGCGCT AGC^GCT
3523 AGCGCT
Eco81I 3018 CCTGAGG CC^T[ACGT]AGG
3141 CCTGAGG
HindIII 87 AAGCTT A^AGCTT
396 AAGCTT
MaeII 2464 ACGT A^CGT
2983 ACGT
RcaI 557 TCATGA T^CATGA
1531 TCATGA
SmlI 812 CTTGAG C^T[CT][GA]AG
2244 CTTGAG
Tsp509I 2686 AATT ^AATT
2861 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbsI BbvCI BbvI
BclI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspEI BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI