##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI BamHI
BbvCI BclI BcnI BfrI BglI
BlpI Bpu1102I BsiWI Bsp1407I BspHI
BsrBI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI DrdI Eam1105I
Eco47III Eco81I EcoRI FbaI FseI
KpnI MluI NaeI NciI NgoMIV
NotI NruI NspV PacI PinAI
PmeI PshAI PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 2648 TCCGGA T^CCGGA
AclI 1687 AACGTT AA^CGTT
AlwNI 2204 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3112 ATTAAT AT^TAAT
AsnI 3112 ATTAAT AT^TAAT
AspI 2194 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 820 TGCGCA TGC^GCA
AvrII 2972 CCTAGG C^CTAGG
BbeI 1813 GGCGCC GGCGC^C
BbrPI 1275 CACGTG CAC^GTG
BcgI 2795 GCAAGTTATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2219 GGATAC GTATCC(6/5)
BglII 1370 AGATCT A^GATCT
BlnI 2972 CCTAGG C^CTAGG
BsaI 291 GAGACC GGTCTC(1/5)
BsaWI 2648 TCCGGA [AT]^CCGG[AT]
BseAI 2648 TCCGGA T^CCGGA
BspDI 429 ATCGAT AT^CGAT
BspEI 2648 TCCGGA T^CCGGA
BspLU11I 3170 ACATGT A^CATGT
BsrFI 18 GCCGGT [GA]^CCGG[CT]
BssHII 60 GCGCGC G^CGCGC
BssSI 2465 CACGAG CACGAG(-5/-1)
BstEII 1639 GGTTACC G^GT[ACGT]ACC
BstPI 1639 GGTTACC G^GT[ACGT]ACC
BstXI 199 CCAAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 18 GCCGGT [GA]^CCGG[CT]
ClaI 429 ATCGAT AT^CGAT
DraI 3087 TTTAAA TTT^AAA
DraIII 1966 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 16 CGGCCG C^GGCCG
EclXI 16 CGGCCG C^GGCCG
Eco52I 16 CGGCCG C^GGCCG
EcoNI 1664 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1639 GGTTACC G^GT[ACGT]ACC
EcoRV 2762 GATATC GAT^ATC
FspI 820 TGCGCA TGC^GCA
HindIII 897 AAGCTT A^AGCTT
HpaI 950 GTTAAC GTT^AAC
KasI 1813 GGCGCC G^GCGCC
MroI 2648 TCCGGA T^CCGGA
NarI 1813 GGCGCC GG^CGCC
NcoI 205 CCATGG C^CATGG
NheI 1820 GCTAGC G^CTAGC
PaeR7I 3467 CTCGAG C^TCGAG
PflFI 2194 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 891 GAGTC GAGTC(4/5)
PmaCI 1275 CACGTG CAC^GTG
PmlI 1275 CACGTG CAC^GTG
PshBI 3112 ATTAAT AT^TAAT
Psp1406I 1687 AACGTT AA^CGTT
SfoI 1813 GGCGCC GGC^GCC
SphI 2897 GCATGC GCATG^C
SspI 825 AATATT AAT^ATT
Tth111I 2194 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3467 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 1813 GGCGCC G[GA]^CG[CT]C
2985 GGCGTC
Asp700I 1950 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3317 GAAATGTTTC
AspHI 282 GAGCAC G[AT]GC[AT]^C
2323 GTGCTC
BanI 1813 GGCGCC G^G[CT][GA]CC
2976 GGCACC
BanII 315 GAGCCC G[GA]GC[CT]^C
2517 GAGCCC
BbsI 1320 GAAGAC GAAGAC(2/6)
3100 GTCTTC
BpuAI 1320 GAAGAC GAAGAC(2/6)
3100 GTCTTC
BsaBI 2724 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3297 GATGGACATC
BsaHI 1813 GGCGCC G[GA]^CG[CT]C
2985 GGCGTC
BsgI 146 GTGCAG GTGCAG(16/14)
335 GTGCAG
BsiEI 16 CGGCCG CG[GA][CT]^CG
20 CGGTCG
BsiHKAI 282 GAGCAC G[AT]GC[AT]^C
2323 GTGCTC
BsmBI 252 GAGACG CGTCTC(1/5)
595 GAGACG
BstAPI 1088 GCAGCTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3033 GCAGATTATGC
HapII 19 CCGG C^CGG
2649 CCGG
HgaI 1703 GCGTC GACGC(5/10)
2986 GCGTC
Hin1I 1813 GGCGCC G[GA]^CG[CT]C
2985 GGCGTC
HincII 504 GTCAAC GT[CT]^[GA]AC
950 GTTAAC
HindII 504 GTCAAC GT[CT]^[GA]AC
950 GTTAAC
HpaII 19 CCGG C^CGG
2649 CCGG
KspI 153 CCGCGG CCGC^GG
213 CCGCGG
MamI 2724 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3297 GATGGACATC
MfeI 2935 CAATTG C^AATTG
3028 CAATTG
MslI 1191 CATAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3612 CATGAAAGTG
MspI 19 CCGG C^CGG
2649 CCGG
MunI 2935 CAATTG C^AATTG
3028 CAATTG
NdeI 2402 CATATG CA^TATG
3414 CATATG
PflMI 1287 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2410 CCAGTGAGTGG
PstI 219 CTGCAG CTGCA^G
2031 CTGCAG
SacII 153 CCGCGG CCGC^GG
213 CCGCGG
ScaI 652 AGTACT AGT^ACT
2038 AGTACT
SfcI 219 CTGCAG C^T[GA][CT]AG
2031 CTGCAG
Tsp45I 580 GTGAC ^GT[GC]AC
3253 GTGAC
Van91I 1287 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2410 CCAGTGAGTGG
XmnI 1950 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3317 GAAATGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaI AvaII
BalI BbvI BfaI BmyI BpmI
BsaAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HhaI HinP1I HinfI HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MvaI MvnI
MwoI NdeII NlaIII NlaIV NsiI
NspI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII