##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AgeI AhdI ApaI
AscI AseI AsnI AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiWI
BspEI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I CelII
CpoI DraII DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoNI EcoO109I
EcoRI EcoT22I FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 3262 GGTACC G^GTACC
Alw44I 308 GTGCAC G^TGCAC
AocI 58 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2031 AGCGCT AGC^GCT
ApaLI 308 GTGCAC G^TGCAC
Asp700I 1084 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3262 GGTACC G^GTACC
AvaI 1575 CCCGGG C^[CT]CG[GA]G
BanI 3262 GGTACC G^G[CT][GA]CC
BanII 1791 GAGCCC G[GA]GC[CT]^C
BciVI 1945 GGATAC GTATCC(6/5)
BclI 2127 TGATCA T^GATCA
BglII 2576 AGATCT A^GATCT
BsaBI 287 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2937 GGTCTC GGTCTC(1/5)
BsaWI 3 ACCGGA [AT]^CCGG[AT]
BseRI 606 GAGGAG GAGGAG(10/8)
BsiEI 80 CGGTCG CG[GA][CT]^CG
BsmBI 1804 CGTCTC CGTCTC(1/5)
BsmFI 600 GGGAC GGGAC(10/14)
BsoBI 1575 CCCGGG C^[CT]CG[GA]G
BspDI 868 ATCGAT AT^CGAT
BspHI 2941 TCATGA T^CATGA
BsrFI 65 ACCGGC [GA]^CCGG[CT]
BstAPI 1977 GCATAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2390 GGTTACC G^GT[ACGT]ACC
BstPI 2390 GGTTACC G^GT[ACGT]ACC
BstYI 2576 AGATCT [GA]^GATC[CT]
Bsu36I 58 CCTGAGG CC^T[ACGT]AGG
Cfr10I 65 ACCGGC [GA]^CCGG[CT]
ClaI 868 ATCGAT AT^CGAT
DraI 988 TTTAAA TTT^AAA
Eco47III 2031 AGCGCT AGC^GCT
Eco81I 58 CCTGAGG CC^T[ACGT]AGG
EcoO65I 2390 GGTTACC G^GT[ACGT]ACC
EcoRV 709 GATATC GAT^ATC
FbaI 2127 TGATCA T^GATCA
KpnI 3262 GGTACC GGTAC^C
MaeII 117 ACGT A^CGT
MamI 287 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 2576 AGATCT [GA]^GATC[CT]
PstI 1191 CTGCAG CTGCA^G
RcaI 2941 TCATGA T^CATGA
SexAI 370 ACCAGGT A^CC[AT]GGT
SmaI 1575 CCCGGG CCC^GGG
SspI 472 AATATT AAT^ATT
StuI 2820 AGGCCT AGG^CCT
XhoII 2576 AGATCT [GA]^GATC[CT]
XmaCI 1575 CCCGGG C^CCGGG
XmaI 1575 CCCGGG C^CCGGG
XmnI 1084 GAAGTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AflIII 1371 ACATGT A^C[GA][CT]GT
3322 ACATGT
AlwNI 1547 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2217 CAGCAGCTG
AspHI 308 GTGCAC G[AT]GC[AT]^C
2889 GAGCAC
AvaII 63 GGACC G^G[AT]CC
1754 GGACC
BalI 631 TGGCCA TGG^CCA
1242 TGGCCA
BsgI 1556 CTGCAC GTGCAG(16/14)
1337 GTGCAG
BsiHKAI 308 GTGCAC G[AT]GC[AT]^C
2889 GAGCAC
BsmI 1426 GAATGC GAATGC(1/-1)
799 GCATTC
BspLU11I 1371 ACATGT A^CATGT
3322 ACATGT
BspMI 25 GCAGGT ACCTGC(4/8)
2387 GCAGGT
BstXI 1452 CCATGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1744 CCATGAAGATGG
CfoI 55 GCGC GCG^C
2032 GCGC
FauI 32 CCCGC CCCGC(4/6)
1628 CCCGC
HaeII 54 AGCGCC [GA]GCGC^[CT]
2031 AGCGCT
HgaI 777 GCGTC GACGC(5/10)
1803 GCGTC
HhaI 55 GCGC GCG^C
2032 GCGC
HinP1I 55 GCGC G^CGC
2032 GCGC
MluNI 631 TGGCCA TGG^CCA
1242 TGGCCA
MscI 631 TGGCCA TGG^CCA
1242 TGGCCA
PflMI 804 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2647 CCATGGCATGG
SapI 1972 GAAGAGC GCTCTTC(1/4)
2024 GAAGAGC
Van91I 804 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2647 CCATGGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI