##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BanI BbeI BbrPI
BcgI BciVI BcnI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmFI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 2382 CTTAAG C^TTAAG
AflIII 750 ACATGT A^C[GA][CT]GT
AseI 153 ATTAAT AT^TAAT
AsnI 153 ATTAAT AT^TAAT
BamHI 3204 GGATCC G^GATCC
BanII 290 GAGCTC G[GA]GC[CT]^C
BbvCI 1365 GCTGAGG CCTCAGC(-5/-2)
BfrI 2382 CTTAAG C^TTAAG
BsaBI 1521 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2399 GAGACG CGTCTC(1/5)
BsmI 2662 GCATTC GAATGC(1/-1)
BspLU11I 750 ACATGT A^CATGT
DraI 191 TTTAAA TTT^AAA
DraII 3026 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 2477 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 3271 CCATGG C^C[GA][CT]GG
EcoO109I 3026 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2660 ATGCAT ATGCA^T
FauI 204 GCGGG CCCGC(4/6)
HincII 1091 GTCAAC GT[CT]^[GA]AC
HindII 1091 GTCAAC GT[CT]^[GA]AC
MamI 1521 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 3384 CACGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 3271 CCATGG C^CATGG
NdeI 1150 CATATG CA^TATG
NheI 95 GCTAGC G^CTAGC
NlaIV 3204 GGATCC GG[ACGT]^[ACGT]CC
NsiI 2660 ATGCAT ATGCA^T
PflMI 2672 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 153 ATTAAT AT^TAAT
PstI 2898 CTGCAG CTGCA^G
SacI 290 GAGCTC GAGCT^C
ScaI 3436 AGTACT AGT^ACT
Van91I 2672 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2579 CCATCAAGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 762 GTAC GT^AC
3437 GTAC
AocI 978 CCTAAGG CC^T[ACGT]AGG
2877 CCTCAGG
AspHI 290 GAGCTC G[AT]GC[AT]^C
3301 GTGCTC
BclI 1526 TGATCA T^GATCA
2929 TGATCA
BfaI 96 CTAG C^TAG
3564 CTAG
BpmI 2647 CTCCAG CTGGAG(16/14)
1948 CTGGAG
BsgI 2541 GTGCAG GTGCAG(16/14)
3259 GTGCAG
BsiHKAI 290 GAGCTC G[AT]GC[AT]^C
3301 GTGCTC
BspMI 2543 GCAGGT ACCTGC(4/8)
3495 GCAGGT
BstYI 1761 GGATCT [GA]^GATC[CT]
3204 GGATCC
Bsu36I 978 CCTAAGG CC^T[ACGT]AGG
2877 CCTCAGG
Eco81I 978 CCTAAGG CC^T[ACGT]AGG
2877 CCTCAGG
EcoNI 104 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2310 CCTGGAAAAGG
FbaI 1526 TGATCA T^GATCA
2929 TGATCA
MaeI 96 CTAG C^TAG
3564 CTAG
MflI 1761 GGATCT [GA]^GATC[CT]
3204 GGATCC
MspA1I 2091 CAGCGG C[AC]G^C[GT]G
2139 CAGCGG
NspI 750 ACATGT [GA]CATG^[CT]
2893 ACATGC
RsaI 762 GTAC GT^AC
3437 GTAC
SspI 1234 AATATT AAT^ATT
2999 AATATT
TaqI 611 TCGA T^CGA
655 TCGA
TthHB8I 611 TCGA T^CGA
655 TCGA
XhoII 1761 GGATCT [GA]^GATC[CT]
3204 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI Asp700I BbsI BbvI BmyI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HindIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII PleI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI