################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BcnI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflII 2382 CTTAAG C^TTAAG AflIII 750 ACATGT A^C[GA][CT]GT AseI 153 ATTAAT AT^TAAT AsnI 153 ATTAAT AT^TAAT BamHI 3204 GGATCC G^GATCC BanII 290 GAGCTC G[GA]GC[CT]^C BbvCI 1365 GCTGAGG CCTCAGC(-5/-2) BfrI 2382 CTTAAG C^TTAAG BsaBI 1521 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2399 GAGACG CGTCTC(1/5) BsmI 2662 GCATTC GAATGC(1/-1) BspLU11I 750 ACATGT A^CATGT DraI 191 TTTAAA TTT^AAA DraII 3026 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 2477 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 3271 CCATGG C^C[GA][CT]GG EcoO109I 3026 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoT22I 2660 ATGCAT ATGCA^T FauI 204 GCGGG CCCGC(4/6) HincII 1091 GTCAAC GT[CT]^[GA]AC HindII 1091 GTCAAC GT[CT]^[GA]AC MamI 1521 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 3384 CACGAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 3271 CCATGG C^CATGG NdeI 1150 CATATG CA^TATG NheI 95 GCTAGC G^CTAGC NlaIV 3204 GGATCC GG[ACGT]^[ACGT]CC NsiI 2660 ATGCAT ATGCA^T PflMI 2672 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 153 ATTAAT AT^TAAT PstI 2898 CTGCAG CTGCA^G SacI 290 GAGCTC GAGCT^C ScaI 3436 AGTACT AGT^ACT Van91I 2672 CCACTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2579 CCATCAAGTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 762 GTAC GT^AC 3437 GTAC AocI 978 CCTAAGG CC^T[ACGT]AGG 2877 CCTCAGG AspHI 290 GAGCTC G[AT]GC[AT]^C 3301 GTGCTC BclI 1526 TGATCA T^GATCA 2929 TGATCA BfaI 96 CTAG C^TAG 3564 CTAG BpmI 2647 CTCCAG CTGGAG(16/14) 1948 CTGGAG BsgI 2541 GTGCAG GTGCAG(16/14) 3259 GTGCAG BsiHKAI 290 GAGCTC G[AT]GC[AT]^C 3301 GTGCTC BspMI 2543 GCAGGT ACCTGC(4/8) 3495 GCAGGT BstYI 1761 GGATCT [GA]^GATC[CT] 3204 GGATCC Bsu36I 978 CCTAAGG CC^T[ACGT]AGG 2877 CCTCAGG Eco81I 978 CCTAAGG CC^T[ACGT]AGG 2877 CCTCAGG EcoNI 104 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2310 CCTGGAAAAGG FbaI 1526 TGATCA T^GATCA 2929 TGATCA MaeI 96 CTAG C^TAG 3564 CTAG MflI 1761 GGATCT [GA]^GATC[CT] 3204 GGATCC MspA1I 2091 CAGCGG C[AC]G^C[GT]G 2139 CAGCGG NspI 750 ACATGT [GA]CATG^[CT] 2893 ACATGC RsaI 762 GTAC GT^AC 3437 GTAC SspI 1234 AATATT AAT^ATT 2999 AATATT TaqI 611 TCGA T^CGA 655 TCGA TthHB8I 611 TCGA T^CGA 655 TCGA XhoII 1761 GGATCT [GA]^GATC[CT] 3204 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI Asp700I BbsI BbvI BmyI BpuAI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI