##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfrI BlnI BsaAI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeII MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Alw44I 1888 GTGCAC G^TGCAC
ApaLI 1888 GTGCAC G^TGCAC
AspI 108 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 702 GGACC G^G[AT]CC
BanI 3217 GGCACC G^G[CT][GA]CC
BanII 2453 GAGCTC G[GA]GC[CT]^C
BbsI 2822 GAAGAC GAAGAC(2/6)
BbvI 2865 GCTGC GCAGC(8/12)
BglI 3209 GCCTCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3471 AGATCT A^GATCT
BlpI 1286 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1286 GCTCAGC GC^T[ACGT]AGC
BpuAI 2822 GAAGAC GAAGAC(2/6)
BsaWI 203 ACCGGA [AT]^CCGG[AT]
BseRI 163 GAGGAG GAGGAG(10/8)
BsmFI 114 GTCCC GGGAC(10/14)
BstAPI 1120 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 73 GCAGGC GC[ACGT]^[ACGT]GC
CelII 1286 GCTCAGC GC^T[ACGT]AGC
DraII 75 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 301 CCATGG C^C[GA][CT]GG
EcoO109I 75 AGGCCCC [GA]G^G[ACGT]CC[CT]
Fnu4HI 2865 GCTGC GC^[ACGT]GC
HapII 204 CCGG C^CGG
HpaII 204 CCGG C^CGG
ItaI 2865 GCTGC GC^[ACGT]GC
MfeI 3308 CAATTG C^AATTG
MspI 204 CCGG C^CGG
MunI 3308 CAATTG C^AATTG
NcoI 301 CCATGG C^CATGG
PflFI 108 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 112 GAGTC GAGTC(4/5)
PstI 71 CTGCAG CTGCA^G
SacI 2453 GAGCTC GAGCT^C
SapI 1129 GCTCTTC GCTCTTC(1/4)
ScaI 3487 AGTACT AGT^ACT
SmlI 2450 CTTGAG C^T[CT][GA]AG
TseI 2865 GCTGC G^C[AT]GC
Tth111I 108 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3510 CCATATATGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflIII 1218 ACATGT A^C[GA][CT]GT
3168 ACATGT
BamHI 180 GGATCC G^GATCC
3506 GGATCC
BsaBI 361 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3241 GATATATATC
BsiYI 2198 CCATCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3280 CCCAAAATTGG
BslI 2198 CCATCTGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
3280 CCCAAAATTGG
BsmAI 330 GAGAC GTCTC(1/5)
393 GAGAC
Bsp1407I 1299 TGTACA T^GTACA
3229 TGTACA
BspHI 966 TCATGA T^CATGA
1554 TCATGA
BspLU11I 1218 ACATGT A^CATGT
3168 ACATGT
BsrGI 1299 TGTACA T^GTACA
3229 TGTACA
BssKI 117 CCAGG ^CC[ACGT]GG
378 CCAGG
BstNI 117 CCAGG CC^[AT]GG
378 CCAGG
Cfr13I 76 GGCCC G^G[ACGT]CC
702 GGACC
EcoRII 117 CCAGG ^CC[AT]GG
378 CCAGG
EcoT14I 301 CCATGG C^C[AT][AT]GG
2317 CCAAGG
EcoT22I 914 ATGCAT ATGCA^T
1082 ATGCAT
HindIII 851 AAGCTT A^AGCTT
1439 AAGCTT
MamI 361 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3241 GATATATATC
MvaI 117 CCAGG CC^[AT]GG
378 CCAGG
MwoI 1120 GCATCCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
3209 GCCTCACAGGC
NsiI 914 ATGCAT ATGCA^T
1082 ATGCAT
RcaI 966 TCATGA T^CATGA
1554 TCATGA
Sau96I 76 GGCCC G^G[ACGT]CC
702 GGACC
ScrFI 117 CCAGG CC^[ACGT]GG
378 CCAGG
SspBI 1299 TGTACA T^GTACA
3229 TGTACA
StyI 301 CCATGG C^C[AT][AT]GG
2317 CCAAGG
TfiI 3130 GAATC G^A[AT]TC
3262 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI BclI BfaI BmyI
BpmI BsaJI BsiHKAI BsmI Bsp1286I
BsrDI BsrI BstF5I BstYI DdeI
DpnII DraI EarI EcoRI FbaI
FokI HaeIII HinfI HphI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI NdeI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
SfaNI SfcI SspI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI