##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AviII AvrII BalI BbrPI
BbvCI BciVI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsiWI Bsp1407I
BspDI BspHI BspLU11I BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DrdI EaeI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FseI
FspI KspI MamI MfeI MluI
MluNI MscI MunI NaeI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2496 GGTACC G^GTACC
AccI 2132 GTAGAC GT^[AC][GT]AC
AccII 279 CGCG CG^CG
AccIII 205 TCCGGA T^CCGGA
AocI 735 CCTCAGG CC^T[ACGT]AGG
ApaI 108 GGGCCC GGGCC^C
Asp700I 550 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2496 GGTACC G^GTACC
AspHI 1181 GAGCAC G[AT]GC[AT]^C
AspI 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 47 GGATCC G^GATCC
BbeI 303 GGCGCC GGCGC^C
BcgI 2845 GCATGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 964 TGATCA T^GATCA
BglI 257 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 569 AGATCT A^GATCT
BseAI 205 TCCGGA T^CCGGA
BsiHKAI 1181 GAGCAC G[AT]GC[AT]^C
BsmBI 2912 GAGACG CGTCTC(1/5)
BspEI 205 TCCGGA T^CCGGA
BspMI 776 GCAGGT ACCTGC(4/8)
BsrBI 124 GAGCGG CCGCTC(-3/-3)
BsrDI 3307 GCAATG GCAATG(2/0)
BstUI 279 CGCG CG^CG
Bsu36I 735 CCTCAGG CC^T[ACGT]AGG
DraI 3339 TTTAAA TTT^AAA
DraIII 2016 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 735 CCTCAGG CC^T[ACGT]AGG
EcoNI 2096 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2725 GAATTC G^AATTC
EcoT22I 1281 ATGCAT ATGCA^T
FbaI 964 TGATCA T^GATCA
HaeII 303 GGCGCC [GA]GCGC^[CT]
HindIII 581 AAGCTT A^AGCTT
HpaI 68 GTTAAC GTT^AAC
KasI 303 GGCGCC G^GCGCC
KpnI 2496 GGTACC GGTAC^C
MaeII 892 ACGT A^CGT
MroI 205 TCCGGA T^CCGGA
MvnI 279 CGCG CG^CG
NarI 303 GGCGCC GG^CGCC
NheI 2141 GCTAGC G^CTAGC
NsiI 1281 ATGCAT ATGCA^T
NspI 2148 GCATGC [GA]CATG^[CT]
PflFI 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1149 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2 CTGCAG CTGCA^G
SapI 2090 GAAGAGC GCTCTTC(1/4)
SfoI 303 GGCGCC GGC^GCC
SmaI 105 CCCGGG CCC^GGG
SphI 2148 GCATGC GCATG^C
Tth111I 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1766 CCAACAAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 105 CCCGGG C^CCGGG
XmaI 105 CCCGGG C^CCGGG
XmnI 550 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AhdI 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2863 GACTATTAGTC
AlwNI 1287 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2082 CAGCTGCTG
AspEI 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2863 GACTATTAGTC
BsaI 3317 GAGACC GGTCTC(1/5)
951 GGTCTC
BsgI 2013 CTGCAC GTGCAG(16/14)
293 GTGCAG
BsiEI 113 CGACCG CG[GA][CT]^CG
817 CGACCG
BsmI 274 GAATGC GAATGC(1/-1)
1336 GCATTC
BssSI 2608 CACGAG CACGAG(-5/-1)
2097 CTCGTG
BstXI 1195 CCAATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3249 CCAGCTATTTGG
Eam1105I 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2863 GACTATTAGTC
HincII 68 GTTAAC GT[CT]^[GA]AC
2257 GTCAAC
HindII 68 GTTAAC GT[CT]^[GA]AC
2257 GTCAAC
PflMI 322 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1270 CCAACCACTGG
PpuMI 697 AGGACCC [GA]G^G[AT]CC[CT]
1005 GGGACCC
SmlI 1860 CTCAAG C^T[CT][GA]AG
2185 CTCAAG
Van91I 322 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1270 CCAACCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII