################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII AclI AflII AflIII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AviII AvrII BalI BbrPI BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DrdI EaeI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI KspI MamI MfeI MluI MluNI MscI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 2496 GGTACC G^GTACC AccI 2132 GTAGAC GT^[AC][GT]AC AccII 279 CGCG CG^CG AccIII 205 TCCGGA T^CCGGA AocI 735 CCTCAGG CC^T[ACGT]AGG ApaI 108 GGGCCC GGGCC^C Asp700I 550 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2496 GGTACC G^GTACC AspHI 1181 GAGCAC G[AT]GC[AT]^C AspI 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 47 GGATCC G^GATCC BbeI 303 GGCGCC GGCGC^C BcgI 2845 GCATGGCTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 964 TGATCA T^GATCA BglI 257 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 569 AGATCT A^GATCT BseAI 205 TCCGGA T^CCGGA BsiHKAI 1181 GAGCAC G[AT]GC[AT]^C BsmBI 2912 GAGACG CGTCTC(1/5) BspEI 205 TCCGGA T^CCGGA BspMI 776 GCAGGT ACCTGC(4/8) BsrBI 124 GAGCGG CCGCTC(-3/-3) BsrDI 3307 GCAATG GCAATG(2/0) BstUI 279 CGCG CG^CG Bsu36I 735 CCTCAGG CC^T[ACGT]AGG DraI 3339 TTTAAA TTT^AAA DraIII 2016 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 735 CCTCAGG CC^T[ACGT]AGG EcoNI 2096 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2725 GAATTC G^AATTC EcoT22I 1281 ATGCAT ATGCA^T FbaI 964 TGATCA T^GATCA HaeII 303 GGCGCC [GA]GCGC^[CT] HindIII 581 AAGCTT A^AGCTT HpaI 68 GTTAAC GTT^AAC KasI 303 GGCGCC G^GCGCC KpnI 2496 GGTACC GGTAC^C MaeII 892 ACGT A^CGT MroI 205 TCCGGA T^CCGGA MvnI 279 CGCG CG^CG NarI 303 GGCGCC GG^CGCC NheI 2141 GCTAGC G^CTAGC NsiI 1281 ATGCAT ATGCA^T NspI 2148 GCATGC [GA]CATG^[CT] PflFI 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1149 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2 CTGCAG CTGCA^G SapI 2090 GAAGAGC GCTCTTC(1/4) SfoI 303 GGCGCC GGC^GCC SmaI 105 CCCGGG CCC^GGG SphI 2148 GCATGC GCATG^C Tth111I 3319 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1766 CCAACAAACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 105 CCCGGG C^CCGGG XmaI 105 CCCGGG C^CCGGG XmnI 550 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AhdI 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2863 GACTATTAGTC AlwNI 1287 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 2082 CAGCTGCTG AspEI 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2863 GACTATTAGTC BsaI 3317 GAGACC GGTCTC(1/5) 951 GGTCTC BsgI 2013 CTGCAC GTGCAG(16/14) 293 GTGCAG BsiEI 113 CGACCG CG[GA][CT]^CG 817 CGACCG BsmI 274 GAATGC GAATGC(1/-1) 1336 GCATTC BssSI 2608 CACGAG CACGAG(-5/-1) 2097 CTCGTG BstXI 1195 CCAATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3249 CCAGCTATTTGG Eam1105I 818 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2863 GACTATTAGTC HincII 68 GTTAAC GT[CT]^[GA]AC 2257 GTCAAC HindII 68 GTTAAC GT[CT]^[GA]AC 2257 GTCAAC PflMI 322 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1270 CCAACCACTGG PpuMI 697 AGGACCC [GA]G^G[AT]CC[CT] 1005 GGGACCC SmlI 1860 CTCAAG C^T[CT][GA]AG 2185 CTCAAG Van91I 322 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1270 CCAACCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII