##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AcyI AgeI AhdI
AscI AseI AsnI AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BglI BlnI BsaAI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I CpoI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoT22I FbaI FseI FspI Hin1I
KasI KspI MfeI MluI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI Tth111I
XbaI XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1784 GGTACC G^GTACC
AccIII 2533 TCCGGA T^CCGGA
AflIII 3080 ACATGT A^C[GA][CT]GT
Alw44I 2455 GTGCAC G^TGCAC
AlwNI 1177 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2711 CCTTAGG CC^T[ACGT]AGG
Aor51HI 113 AGCGCT AGC^GCT
ApaI 104 GGGCCC GGGCC^C
ApaLI 2455 GTGCAC G^TGCAC
Asp718I 1784 GGTACC G^GTACC
BamHI 3237 GGATCC G^GATCC
BanI 1784 GGTACC G^G[CT][GA]CC
BciVI 222 GGATAC GTATCC(6/5)
BcnI 132 CCCGG CC^[GC]GG
BglII 734 AGATCT A^GATCT
BsaBI 1374 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2732 GAGACC GGTCTC(1/5)
BsaWI 2533 TCCGGA [AT]^CCGG[AT]
BseAI 2533 TCCGGA T^CCGGA
BspDI 2328 ATCGAT AT^CGAT
BspEI 2533 TCCGGA T^CCGGA
BspLU11I 3080 ACATGT A^CATGT
BsrDI 3309 GCAATG GCAATG(2/0)
BstXI 1658 CCAAATAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2711 CCTTAGG CC^T[ACGT]AGG
ClaI 2328 ATCGAT AT^CGAT
DsaI 2131 CCACGG C^C[GA][CT]GG
Eco47III 113 AGCGCT AGC^GCT
Eco81I 2711 CCTTAGG CC^T[ACGT]AGG
EcoNI 2460 CCTAAGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1307 GAATTC G^AATTC
EcoRV 566 GATATC GAT^ATC
EcoT14I 126 CCAAGG C^C[AT][AT]GG
FauI 2584 GCGGG CCCGC(4/6)
HgaI 122 GCGTC GACGC(5/10)
HpaI 2904 GTTAAC GTT^AAC
KpnI 1784 GGTACC GGTAC^C
MamI 1374 GATAACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2533 TCCGGA T^CCGGA
NciI 132 CCCGG CC^[GC]GG
NdeI 2916 CATATG CA^TATG
PaeR7I 117 CTCGAG C^TCGAG
PflMI 2142 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 584 AGGTCCT [GA]G^G[AT]CC[CT]
SspI 712 AATATT AAT^ATT
StuI 1997 AGGCCT AGG^CCT
StyI 126 CCAAGG C^C[AT][AT]GG
SwaI 3355 ATTTAAAT ATTT^AAAT
Van91I 2142 CCAACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 117 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 69 CGCG CG^CG
2761 CGCG
AflII 2517 CTTAAG C^TTAAG
2847 CTTAAG
AvaI 117 CTCGAG C^[CT]CG[GA]G
1527 CCCGAG
BalI 2544 TGGCCA TGG^CCA
2572 TGGCCA
BfrI 2517 CTTAAG C^TTAAG
2847 CTTAAG
BsoBI 117 CTCGAG C^[CT]CG[GA]G
1527 CCCGAG
BspHI 650 TCATGA T^CATGA
1042 TCATGA
BstUI 69 CGCG CG^CG
2761 CGCG
CfoI 114 GCGC GCG^C
2677 GCGC
DraII 103 GGGGCCC [GA]G^G[ACGT]CC[CT]
584 AGGTCCT
EaeI 2544 TGGCCA [CT]^GGCC[GA]
2572 TGGCCA
EcoO109I 103 GGGGCCC [GA]G^G[ACGT]CC[CT]
584 AGGTCCT
HaeII 113 AGCGCT [GA]GCGC^[CT]
2676 AGCGCC
HapII 133 CCGG C^CGG
2534 CCGG
HhaI 114 GCGC GCG^C
2677 GCGC
HinP1I 114 GCGC G^CGC
2677 GCGC
HincII 890 GTTGAC GT[CT]^[GA]AC
2904 GTTAAC
HindII 890 GTTGAC GT[CT]^[GA]AC
2904 GTTAAC
HindIII 2362 AAGCTT A^AGCTT
3529 AAGCTT
HpaII 133 CCGG C^CGG
2534 CCGG
MluNI 2544 TGGCCA TGG^CCA
2572 TGGCCA
MscI 2544 TGGCCA TGG^CCA
2572 TGGCCA
MspI 133 CCGG C^CGG
2534 CCGG
MvnI 69 CGCG CG^CG
2761 CGCG
PleI 1523 GACTC GAGTC(4/5)
2115 GACTC
PvuII 146 CAGCTG CAG^CTG
2505 CAGCTG
RcaI 650 TCATGA T^CATGA
1042 TCATGA
SacI 12 GAGCTC GAGCT^C
50 GAGCTC
ScaI 1197 AGTACT AGT^ACT
1227 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AclI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI Psp1406I PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI