################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AvaI AvaII AviII BamHI BanII BbeI BbrPI BciVI BclI BfrI BglI BglII BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII BssSI BstBI BstEII BstPI BstUI BstYI CelII CfoI Cfr10I ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MamI MflI MluI MroI MvnI NaeI NarI NcoI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I StuI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AvrII 666 CCTAGG C^CTAGG BalI 2069 TGGCCA TGG^CCA BanI 2872 GGTGCC G^G[CT][GA]CC BbsI 2846 GTCTTC GAAGAC(2/6) BbvCI 1731 CCTCAGC CCTCAGC(-5/-2) BlnI 666 CCTAGG C^CTAGG BmyI 1759 GGGCAC G[AGT]GC[ACT]^C BpmI 1664 CTGGAG CTGGAG(16/14) BpuAI 2846 GTCTTC GAAGAC(2/6) BsaAI 3169 TACGTG [CT]AC^GT[GA] BseRI 2909 GAGGAG GAGGAG(10/8) Bsp1286I 1759 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 454 TGTACA T^GTACA BspMI 436 ACCTGC ACCTGC(4/8) BsrDI 169 CATTGC GCAATG(2/0) BsrGI 454 TGTACA T^GTACA Bst1107I 478 GTATAC GTA^TAC BstXI 2009 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 478 GTATAC GTA^TAC DraII 1922 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 3103 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 842 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1922 AGGCCCC [GA]G^G[ACGT]CC[CT] FauI 1757 GCGGG CCCGC(4/6) MfeI 1135 CAATTG C^AATTG MluNI 2069 TGGCCA TGG^CCA MscI 2069 TGGCCA TGG^CCA MunI 1135 CAATTG C^AATTG PshAI 208 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2200 CTGCAG CTGCA^G PvuII 2941 CAGCTG CAG^CTG SmlI 189 CTTGAG C^T[CT][GA]AG SspBI 454 TGTACA T^GTACA SwaI 446 ATTTAAAT ATTT^AAAT ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 194 GTAGAC GT^[AC][GT]AC 478 GTATAC AciI 235 GCGG CCGC(-3/-1) 1757 GCGG AfaI 455 GTAC GT^AC 885 GTAC AocI 1857 CCTCAGG CC^T[ACGT]AGG 2042 CCTGAGG AseI 29 ATTAAT AT^TAAT 2455 ATTAAT AsnI 29 ATTAAT AT^TAAT 2455 ATTAAT AspI 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2252 GACTTCGTC BcgI 1720 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2383 CGATATTCCTGC BcnI 1834 CCGGG CC^[GC]GG 2025 CCGGG BsaI 2059 GAGACC GGTCTC(1/5) 1841 GGTCTC BsiYI 545 CCTGTTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 842 CCTTTAATAGG BslI 545 CCTGTTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 842 CCTTTAATAGG BsmBI 2257 CGTCTC CGTCTC(1/5) 1811 GAGACG BspHI 409 TCATGA T^CATGA 3151 TCATGA BstAPI 636 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1695 GCAACCTCTGC Bsu36I 1857 CCTCAGG CC^T[ACGT]AGG 2042 CCTGAGG Cfr13I 1923 GGCCC G^G[ACGT]CC 2670 GGCCC EaeI 1831 TGGCCG [CT]^GGCC[GA] 2069 TGGCCA Eco81I 1857 CCTCAGG CC^T[ACGT]AGG 2042 CCTGAGG HapII 1834 CCGG C^CGG 2025 CCGG HindIII 308 AAGCTT A^AGCTT 2955 AAGCTT HpaII 1834 CCGG C^CGG 2025 CCGG MspA1I 233 CAGCGG C[AC]G^C[GT]G 2941 CAGCTG MspI 1834 CCGG C^CGG 2025 CCGG NciI 1834 CCGGG CC^[GC]GG 2025 CCGGG NheI 3111 GCTAGC G^CTAGC 3128 GCTAGC NlaIV 1923 GGCCCC GG[ACGT]^[ACGT]CC 2872 GGTGCC PflFI 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2252 GACTTCGTC PshBI 29 ATTAAT AT^TAAT 2455 ATTAAT RcaI 409 TCATGA T^CATGA 3151 TCATGA RsaI 455 GTAC GT^AC 885 GTAC Sau96I 1923 GGCCC G^G[ACGT]CC 2670 GGCCC SfaNI 251 GATGC GCATC(5/9) 913 GCATC SphI 2133 GCATGC GCATG^C 3082 GCATGC Tth111I 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC 2252 GACTTCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AcsI AluI AlwI ApoI BbvI BfaI BsaJI BsgI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI EarI EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII NsiI NspI Sau3AI ScrFI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I