##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AvaI AvaII AviII BamHI BanII
BbeI BbrPI BciVI BclI BfrI
BglI BglII BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmI BsoBI BspDI BspEI
BspLU11I BsrBI BsrFI BssHII BssSI
BstBI BstEII BstPI BstUI BstYI
CelII CfoI Cfr10I ClaI CpoI
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI KspI MamI
MflI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PaeR7I PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AvrII 666 CCTAGG C^CTAGG
BalI 2069 TGGCCA TGG^CCA
BanI 2872 GGTGCC G^G[CT][GA]CC
BbsI 2846 GTCTTC GAAGAC(2/6)
BbvCI 1731 CCTCAGC CCTCAGC(-5/-2)
BlnI 666 CCTAGG C^CTAGG
BmyI 1759 GGGCAC G[AGT]GC[ACT]^C
BpmI 1664 CTGGAG CTGGAG(16/14)
BpuAI 2846 GTCTTC GAAGAC(2/6)
BsaAI 3169 TACGTG [CT]AC^GT[GA]
BseRI 2909 GAGGAG GAGGAG(10/8)
Bsp1286I 1759 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 454 TGTACA T^GTACA
BspMI 436 ACCTGC ACCTGC(4/8)
BsrDI 169 CATTGC GCAATG(2/0)
BsrGI 454 TGTACA T^GTACA
Bst1107I 478 GTATAC GTA^TAC
BstXI 2009 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 478 GTATAC GTA^TAC
DraII 1922 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 3103 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 842 CCTTTAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1922 AGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 1757 GCGGG CCCGC(4/6)
MfeI 1135 CAATTG C^AATTG
MluNI 2069 TGGCCA TGG^CCA
MscI 2069 TGGCCA TGG^CCA
MunI 1135 CAATTG C^AATTG
PshAI 208 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2200 CTGCAG CTGCA^G
PvuII 2941 CAGCTG CAG^CTG
SmlI 189 CTTGAG C^T[CT][GA]AG
SspBI 454 TGTACA T^GTACA
SwaI 446 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 194 GTAGAC GT^[AC][GT]AC
478 GTATAC
AciI 235 GCGG CCGC(-3/-1)
1757 GCGG
AfaI 455 GTAC GT^AC
885 GTAC
AocI 1857 CCTCAGG CC^T[ACGT]AGG
2042 CCTGAGG
AseI 29 ATTAAT AT^TAAT
2455 ATTAAT
AsnI 29 ATTAAT AT^TAAT
2455 ATTAAT
AspI 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2252 GACTTCGTC
BcgI 1720 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2383 CGATATTCCTGC
BcnI 1834 CCGGG CC^[GC]GG
2025 CCGGG
BsaI 2059 GAGACC GGTCTC(1/5)
1841 GGTCTC
BsiYI 545 CCTGTTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
842 CCTTTAATAGG
BslI 545 CCTGTTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
842 CCTTTAATAGG
BsmBI 2257 CGTCTC CGTCTC(1/5)
1811 GAGACG
BspHI 409 TCATGA T^CATGA
3151 TCATGA
BstAPI 636 GCATATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1695 GCAACCTCTGC
Bsu36I 1857 CCTCAGG CC^T[ACGT]AGG
2042 CCTGAGG
Cfr13I 1923 GGCCC G^G[ACGT]CC
2670 GGCCC
EaeI 1831 TGGCCG [CT]^GGCC[GA]
2069 TGGCCA
Eco81I 1857 CCTCAGG CC^T[ACGT]AGG
2042 CCTGAGG
HapII 1834 CCGG C^CGG
2025 CCGG
HindIII 308 AAGCTT A^AGCTT
2955 AAGCTT
HpaII 1834 CCGG C^CGG
2025 CCGG
MspA1I 233 CAGCGG C[AC]G^C[GT]G
2941 CAGCTG
MspI 1834 CCGG C^CGG
2025 CCGG
NciI 1834 CCGGG CC^[GC]GG
2025 CCGGG
NheI 3111 GCTAGC G^CTAGC
3128 GCTAGC
NlaIV 1923 GGCCCC GG[ACGT]^[ACGT]CC
2872 GGTGCC
PflFI 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2252 GACTTCGTC
PshBI 29 ATTAAT AT^TAAT
2455 ATTAAT
RcaI 409 TCATGA T^CATGA
3151 TCATGA
RsaI 455 GTAC GT^AC
885 GTAC
Sau96I 1923 GGCCC G^G[ACGT]CC
2670 GGCCC
SfaNI 251 GATGC GCATC(5/9)
913 GCATC
SphI 2133 GCATGC GCATG^C
3082 GCATGC
Tth111I 197 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
2252 GACTTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AcsI AluI AlwI ApoI BbvI
BfaI BsaJI BsgI BsmAI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
DdeI DpnII DraI EarI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MvaI MwoI NdeI
NdeII NlaIII NsiI NspI Sau3AI
ScrFI SfcI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I