################################## # NO CUT ENZYMES ( 108 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanII BbeI BciVI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BssHII BstAPI BstBI BstEII BstPI CelII ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRV FseI FspI HaeII HgaI Hin1I KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflII 1946 CTTAAG C^TTAAG AgeI 479 ACCGGT A^CCGGT Asp700I 2532 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1673 CCCGGG C^[CT]CG[GA]G BanI 1775 GGTGCC G^G[CT][GA]CC BbrPI 786 CACGTG CAC^GTG BcgI 2771 CGAGATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1946 CTTAAG C^TTAAG BglII 2419 AGATCT A^GATCT BmyI 2563 GGGCAC G[AGT]GC[ACT]^C BsaAI 786 CACGTG [CT]AC^GT[GA] BsaWI 479 ACCGGT [AT]^CCGG[AT] BseRI 903 CTCCTC GAGGAG(10/8) BsgI 1359 CTGCAC GTGCAG(16/14) BsoBI 1673 CCCGGG C^[CT]CG[GA]G Bsp1286I 2563 GGGCAC G[AGT]GC[ACT]^C Bsp1407I 2224 TGTACA T^GTACA BspLU11I 3297 ACATGT A^CATGT BspMI 2955 GCAGGT ACCTGC(4/8) BsrBI 900 CCGCTC CCGCTC(-3/-3) BsrGI 2224 TGTACA T^GTACA BssSI 2965 CACGAG CACGAG(-5/-1) BstYI 2419 AGATCT [GA]^GATC[CT] CfoI 3055 GCGC GCG^C EcoRI 1181 GAATTC G^AATTC HhaI 3055 GCGC GCG^C HinP1I 3055 GCGC G^CGC HincII 3446 GTTAAC GT[CT]^[GA]AC HindII 3446 GTTAAC GT[CT]^[GA]AC HpaI 3446 GTTAAC GTT^AAC MflI 2419 AGATCT [GA]^GATC[CT] NaeI 3051 GCCGGC GCC^GGC NgoMIV 3051 GCCGGC G^CCGGC NlaIV 1775 GGTGCC GG[ACGT]^[ACGT]CC PflMI 1311 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 479 ACCGGT A^CCGGT PmaCI 786 CACGTG CAC^GTG PmlI 786 CACGTG CAC^GTG PstI 989 CTGCAG CTGCA^G ScaI 563 AGTACT AGT^ACT SmaI 1673 CCCGGG CCC^GGG SspBI 2224 TGTACA T^GTACA SspI 276 AATATT AAT^ATT StuI 993 AGGCCT AGG^CCT Van91I 1311 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 2419 AGATCT [GA]^GATC[CT] XmaCI 1673 CCCGGG C^CCGGG XmaI 1673 CCCGGG C^CCGGG XmnI 2532 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 41 enzymes) # ################################### AccI 1062 GTATAC GT^[AC][GT]AC 2891 GTATAC AccII 2432 CGCG CG^CG 3056 CGCG AocI 1443 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG AseI 2253 ATTAAT AT^TAAT 3355 ATTAAT AsnI 2253 ATTAAT AT^TAAT 3355 ATTAAT AvaII 482 GGTCC G^G[AT]CC 1154 GGTCC BalI 103 TGGCCA TGG^CCA 2992 TGGCCA BbsI 341 GAAGAC GAAGAC(2/6) 2366 GAAGAC BbvCI 1746 CCTCAGC CCTCAGC(-5/-2) 3082 GCTGAGG BclI 2 TGATCA T^GATCA 3312 TGATCA BcnI 1673 CCCGG CC^[GC]GG 1674 CCGGG BfaI 518 CTAG C^TAG 572 CTAG BpuAI 341 GAAGAC GAAGAC(2/6) 2366 GAAGAC BsaI 1427 GGTCTC GGTCTC(1/5) 1846 GGTCTC BsrFI 479 ACCGGT [GA]^CCGG[CT] 3051 GCCGGC Bst1107I 1062 GTATAC GTA^TAC 2891 GTATAC BstUI 2432 CGCG CG^CG 3056 CGCG BstZ17I 1062 GTATAC GTA^TAC 2891 GTATAC Bsu36I 1443 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG Cfr10I 479 ACCGGT [GA]^CCGG[CT] 3051 GCCGGC Cfr13I 482 GGTCC G^G[ACGT]CC 1154 GGTCC Eco81I 1443 CCTCAGG CC^T[ACGT]AGG 1862 CCTCAGG EcoT14I 2605 CCAAGG C^C[AT][AT]GG 2950 CCAAGG FauI 899 CCCGC CCCGC(4/6) 2430 CCCGC FbaI 2 TGATCA T^GATCA 3312 TGATCA MaeI 518 CTAG C^TAG 572 CTAG MaeII 787 ACGT A^CGT 1207 ACGT MluNI 103 TGGCCA TGG^CCA 2992 TGGCCA MscI 103 TGGCCA TGG^CCA 2992 TGGCCA MspA1I 2903 CAGCTG C[AC]G^C[GT]G 3068 CAGCTG MvnI 2432 CGCG CG^CG 3056 CGCG NciI 1673 CCCGG CC^[GC]GG 1674 CCGGG PleI 3175 GACTC GAGTC(4/5) 2767 GAGTC PshBI 2253 ATTAAT AT^TAAT 3355 ATTAAT PvuII 2903 CAGCTG CAG^CTG 3068 CAGCTG Sau96I 482 GGTCC G^G[ACGT]CC 1154 GGTCC SfcI 989 CTGCAG C^T[GA][CT]AG 1679 CTGTAG SmlI 1946 CTTAAG C^T[CT][GA]AG 2212 CTTGAG StyI 2605 CCAAGG C^C[AT][AT]GG 2950 CCAAGG TaqI 823 TCGA T^CGA 2770 TCGA TthHB8I 823 TCGA T^CGA 2770 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI BbvI BpmI BsaJI BsiYI BslI BsmAI BsrDI BsrI BssKI BstF5I BstNI BstXI Cac8I DdeI DpnII DraI EaeI EarI EcoRII EcoT22I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NsiI NspI RsaI Sau3AI ScrFI SfaNI TfiI Tru9I TseI Tsp45I Tsp509I TspRI