##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BciVI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BspDI BspEI BspHI BssHII BstAPI
BstBI BstEII BstPI CelII ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRV FseI
FspI HaeII HgaI Hin1I KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmeI PpuMI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflII 1946 CTTAAG C^TTAAG
AgeI 479 ACCGGT A^CCGGT
Asp700I 2532 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1673 CCCGGG C^[CT]CG[GA]G
BanI 1775 GGTGCC G^G[CT][GA]CC
BbrPI 786 CACGTG CAC^GTG
BcgI 2771 CGAGATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1946 CTTAAG C^TTAAG
BglII 2419 AGATCT A^GATCT
BmyI 2563 GGGCAC G[AGT]GC[ACT]^C
BsaAI 786 CACGTG [CT]AC^GT[GA]
BsaWI 479 ACCGGT [AT]^CCGG[AT]
BseRI 903 CTCCTC GAGGAG(10/8)
BsgI 1359 CTGCAC GTGCAG(16/14)
BsoBI 1673 CCCGGG C^[CT]CG[GA]G
Bsp1286I 2563 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 2224 TGTACA T^GTACA
BspLU11I 3297 ACATGT A^CATGT
BspMI 2955 GCAGGT ACCTGC(4/8)
BsrBI 900 CCGCTC CCGCTC(-3/-3)
BsrGI 2224 TGTACA T^GTACA
BssSI 2965 CACGAG CACGAG(-5/-1)
BstYI 2419 AGATCT [GA]^GATC[CT]
CfoI 3055 GCGC GCG^C
EcoRI 1181 GAATTC G^AATTC
HhaI 3055 GCGC GCG^C
HinP1I 3055 GCGC G^CGC
HincII 3446 GTTAAC GT[CT]^[GA]AC
HindII 3446 GTTAAC GT[CT]^[GA]AC
HpaI 3446 GTTAAC GTT^AAC
MflI 2419 AGATCT [GA]^GATC[CT]
NaeI 3051 GCCGGC GCC^GGC
NgoMIV 3051 GCCGGC G^CCGGC
NlaIV 1775 GGTGCC GG[ACGT]^[ACGT]CC
PflMI 1311 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 479 ACCGGT A^CCGGT
PmaCI 786 CACGTG CAC^GTG
PmlI 786 CACGTG CAC^GTG
PstI 989 CTGCAG CTGCA^G
ScaI 563 AGTACT AGT^ACT
SmaI 1673 CCCGGG CCC^GGG
SspBI 2224 TGTACA T^GTACA
SspI 276 AATATT AAT^ATT
StuI 993 AGGCCT AGG^CCT
Van91I 1311 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2419 AGATCT [GA]^GATC[CT]
XmaCI 1673 CCCGGG C^CCGGG
XmaI 1673 CCCGGG C^CCGGG
XmnI 2532 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1062 GTATAC GT^[AC][GT]AC
2891 GTATAC
AccII 2432 CGCG CG^CG
3056 CGCG
AocI 1443 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
AseI 2253 ATTAAT AT^TAAT
3355 ATTAAT
AsnI 2253 ATTAAT AT^TAAT
3355 ATTAAT
AvaII 482 GGTCC G^G[AT]CC
1154 GGTCC
BalI 103 TGGCCA TGG^CCA
2992 TGGCCA
BbsI 341 GAAGAC GAAGAC(2/6)
2366 GAAGAC
BbvCI 1746 CCTCAGC CCTCAGC(-5/-2)
3082 GCTGAGG
BclI 2 TGATCA T^GATCA
3312 TGATCA
BcnI 1673 CCCGG CC^[GC]GG
1674 CCGGG
BfaI 518 CTAG C^TAG
572 CTAG
BpuAI 341 GAAGAC GAAGAC(2/6)
2366 GAAGAC
BsaI 1427 GGTCTC GGTCTC(1/5)
1846 GGTCTC
BsrFI 479 ACCGGT [GA]^CCGG[CT]
3051 GCCGGC
Bst1107I 1062 GTATAC GTA^TAC
2891 GTATAC
BstUI 2432 CGCG CG^CG
3056 CGCG
BstZ17I 1062 GTATAC GTA^TAC
2891 GTATAC
Bsu36I 1443 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
Cfr10I 479 ACCGGT [GA]^CCGG[CT]
3051 GCCGGC
Cfr13I 482 GGTCC G^G[ACGT]CC
1154 GGTCC
Eco81I 1443 CCTCAGG CC^T[ACGT]AGG
1862 CCTCAGG
EcoT14I 2605 CCAAGG C^C[AT][AT]GG
2950 CCAAGG
FauI 899 CCCGC CCCGC(4/6)
2430 CCCGC
FbaI 2 TGATCA T^GATCA
3312 TGATCA
MaeI 518 CTAG C^TAG
572 CTAG
MaeII 787 ACGT A^CGT
1207 ACGT
MluNI 103 TGGCCA TGG^CCA
2992 TGGCCA
MscI 103 TGGCCA TGG^CCA
2992 TGGCCA
MspA1I 2903 CAGCTG C[AC]G^C[GT]G
3068 CAGCTG
MvnI 2432 CGCG CG^CG
3056 CGCG
NciI 1673 CCCGG CC^[GC]GG
1674 CCGGG
PleI 3175 GACTC GAGTC(4/5)
2767 GAGTC
PshBI 2253 ATTAAT AT^TAAT
3355 ATTAAT
PvuII 2903 CAGCTG CAG^CTG
3068 CAGCTG
Sau96I 482 GGTCC G^G[ACGT]CC
1154 GGTCC
SfcI 989 CTGCAG C^T[GA][CT]AG
1679 CTGTAG
SmlI 1946 CTTAAG C^T[CT][GA]AG
2212 CTTGAG
StyI 2605 CCAAGG C^C[AT][AT]GG
2950 CCAAGG
TaqI 823 TCGA T^CGA
2770 TCGA
TthHB8I 823 TCGA T^CGA
2770 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI BbvI BpmI BsaJI
BsiYI BslI BsmAI BsrDI BsrI
BssKI BstF5I BstNI BstXI Cac8I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT22I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NsiI NspI
RsaI Sau3AI ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI