##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AvrII BbeI BciVI BlnI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
KasI MluI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI PvuI RcaI
RsrII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1798 GACGTC GACGT^C
Acc65I 2787 GGTACC G^GTACC
AccI 2431 GTCTAC GT^[AC][GT]AC
AccIII 1868 TCCGGA T^CCGGA
AclI 1918 AACGTT AA^CGTT
AcsI 318 GAATTT [GA]^AATT[CT]
AflII 3117 CTTAAG C^TTAAG
ApoI 318 GAATTT [GA]^AATT[CT]
Asp718I 2787 GGTACC G^GTACC
AviII 1049 TGCGCA TGC^GCA
BbrPI 1036 CACGTG CAC^GTG
BbvCI 3065 CCTCAGC CCTCAGC(-5/-2)
BcgI 1052 GCAACCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3117 CTTAAG C^TTAAG
BglII 2577 AGATCT A^GATCT
BsaAI 1036 CACGTG [CT]AC^GT[GA]
BsaBI 879 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1868 TCCGGA [AT]^CCGG[AT]
BseAI 1868 TCCGGA T^CCGGA
BsmI 2622 GAATGC GAATGC(1/-1)
BspEI 1868 TCCGGA T^CCGGA
BsrDI 797 GCAATG GCAATG(2/0)
BstAPI 66 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2243 CCAAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 266 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2154 GACAGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1049 TGCGCA TGC^GCA
HpaI 1921 GTTAAC GTT^AAC
KpnI 2787 GGTACC GGTAC^C
MamI 879 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 504 CAATTG C^AATTG
MroI 1868 TCCGGA T^CCGGA
MunI 504 CAATTG C^AATTG
NaeI 1848 GCCGGC GCC^GGC
NcoI 1670 CCATGG C^CATGG
NgoMIV 1848 GCCGGC G^CCGGC
PaeR7I 171 CTCGAG C^TCGAG
PflMI 2968 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1036 CACGTG CAC^GTG
PmlI 1036 CACGTG CAC^GTG
Psp1406I 1918 AACGTT AA^CGTT
SexAI 713 ACCAGGT A^CC[AT]GGT
SphI 974 GCATGC GCATG^C
Van91I 2968 CCATCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2715 TCTAGA T^CTAGA
XcmI 425 CCAGCTGGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 171 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BamHI 461 GGATCC G^GATCC
2280 GGATCC
BclI 881 TGATCA T^GATCA
1166 TGATCA
BlpI 1387 GCTCAGC GC^T[ACGT]AGC
1939 GCTCAGC
Bpu1102I 1387 GCTCAGC GC^T[ACGT]AGC
1939 GCTCAGC
BsaI 631 GAGACC GGTCTC(1/5)
2422 GAGACC
BspMI 57 ACCTGC ACCTGC(4/8)
1508 ACCTGC
CelII 1387 GCTCAGC GC^T[ACGT]AGC
1939 GCTCAGC
EarI 283 CTCTTC CTCTTC(1/4)
1864 CTCTTC
FbaI 881 TGATCA T^GATCA
1166 TGATCA
HaeII 1356 AGCGCC [GA]GCGC^[CT]
2015 GGCGCT
Ksp632I 283 CTCTTC CTCTTC(1/4)
1864 CTCTTC
PshAI 1794 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2424 GACCGAGGTC
SacI 360 GAGCTC GAGCT^C
1385 GAGCTC
ScaI 590 AGTACT AGT^ACT
2339 AGTACT
SmaI 2570 CCCGGG CCC^GGG
2791 CCCGGG
SmlI 171 CTCGAG C^T[CT][GA]AG
3117 CTTAAG
XmaCI 2570 CCCGGG C^CCGGG
2791 CCCGGG
XmaI 2570 CCCGGG C^CCGGG
2791 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII