##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp718I AspEI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BcgI BfrI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BsrGI BssHII BssSI BstAPI BstEII
BstPI CelII ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I FseI FspI HaeII
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmaCI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI SexAI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 739 GACGTC GACGT^C
AccII 929 CGCG CG^CG
AcyI 739 GACGTC G[GA]^CG[CT]C
AflIII 1473 ACATGT A^C[GA][CT]GT
AocI 363 CCTCAGG CC^T[ACGT]AGG
AseI 2474 ATTAAT AT^TAAT
AsnI 2474 ATTAAT AT^TAAT
Asp700I 1593 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2832 CCTAGG C^CTAGG
BbsI 656 GAAGAC GAAGAC(2/6)
BbvCI 246 CCTCAGC CCTCAGC(-5/-2)
BclI 2192 TGATCA T^GATCA
BglI 339 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2444 AGATCT A^GATCT
BlnI 2832 CCTAGG C^CTAGG
BpuAI 656 GAAGAC GAAGAC(2/6)
BsaAI 101 CACGTA [CT]AC^GT[GA]
BsaHI 739 GACGTC G[GA]^CG[CT]C
BspLU11I 1473 ACATGT A^CATGT
BspMI 964 GCAGGT ACCTGC(4/8)
BsrBI 940 GAGCGG CCGCTC(-3/-3)
BsrFI 2916 GCCGGC [GA]^CCGG[CT]
BstBI 1060 TTCGAA TT^CGAA
BstUI 929 CGCG CG^CG
Bsu36I 363 CCTCAGG CC^T[ACGT]AGG
CfoI 928 GCGC GCG^C
Cfr10I 2916 GCCGGC [GA]^CCGG[CT]
DraIII 1178 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 2344 GAAGAG CTCTTC(1/4)
Eco81I 363 CCTCAGG CC^T[ACGT]AGG
EcoRI 1228 GAATTC G^AATTC
EcoRV 195 GATATC GAT^ATC
EcoT22I 1858 ATGCAT ATGCA^T
FbaI 2192 TGATCA T^GATCA
HgaI 1048 GACGC GACGC(5/10)
HhaI 928 GCGC GCG^C
Hin1I 739 GACGTC G[GA]^CG[CT]C
HinP1I 928 GCGC G^CGC
HpaI 2248 GTTAAC GTT^AAC
Ksp632I 2344 GAAGAG CTCTTC(1/4)
MvnI 929 CGCG CG^CG
NaeI 2916 GCCGGC GCC^GGC
NdeI 2950 CATATG CA^TATG
NgoMIV 2916 GCCGGC G^CCGGC
NsiI 1858 ATGCAT ATGCA^T
NspV 1060 TTCGAA TT^CGAA
PmeI 459 GTTTAAAC GTTT^AAAC
PshBI 2474 ATTAAT AT^TAAT
PstI 3065 CTGCAG CTGCA^G
SalI 898 GTCGAC G^TCGAC
ScaI 2881 AGTACT AGT^ACT
SfiI 338 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1060 TTCGAA TT^CGAA
SphI 1856 GCATGC GCATG^C
SwaI 1081 ATTTAAAT ATTT^AAAT
XbaI 2487 TCTAGA T^CTAGA
XmnI 1593 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AspHI 1175 GAGCAC G[AT]GC[AT]^C
1524 GAGCAC
AspI 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1048 GACGCTGTC
BciVI 501 GTATCC GTATCC(6/5)
2795 GTATCC
BcnI 222 CCCGG CC^[GC]GG
2163 CCCGG
BmyI 1175 GAGCAC G[AGT]GC[ACT]^C
1524 GAGCAC
BpmI 651 CTCCAG CTGGAG(16/14)
2780 CTCCAG
BsgI 421 CTGCAC GTGCAG(16/14)
2042 GTGCAG
BsiHKAI 1175 GAGCAC G[AT]GC[AT]^C
1524 GAGCAC
BsmBI 150 GAGACG CGTCTC(1/5)
317 GAGACG
Bsp1286I 1175 GAGCAC G[AGT]GC[ACT]^C
1524 GAGCAC
BsrDI 646 CATTGC GCAATG(2/0)
185 GCAATG
Bst1107I 727 GTATAC GTA^TAC
3072 GTATAC
BstZ17I 727 GTATAC GTA^TAC
3072 GTATAC
FauI 947 CCCGC CCCGC(4/6)
2219 CCCGC
HindIII 38 AAGCTT A^AGCTT
3015 AAGCTT
MaeII 102 ACGT A^CGT
740 ACGT
NciI 222 CCCGG CC^[GC]GG
2163 CCCGG
NcoI 1537 CCATGG C^CATGG
2157 CCATGG
PflFI 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1048 GACGCTGTC
Tth111I 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1048 GACGCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BbvI BfaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BspHI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII