################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AvaI AviII BamHI BanI BanII BbeI BbrPI BcgI BfrI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BsrGI BssHII BssSI BstAPI BstEII BstPI CelII ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I FseI FspI HaeII KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SapI SbfI SexAI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 739 GACGTC GACGT^C AccII 929 CGCG CG^CG AcyI 739 GACGTC G[GA]^CG[CT]C AflIII 1473 ACATGT A^C[GA][CT]GT AocI 363 CCTCAGG CC^T[ACGT]AGG AseI 2474 ATTAAT AT^TAAT AsnI 2474 ATTAAT AT^TAAT Asp700I 1593 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2832 CCTAGG C^CTAGG BbsI 656 GAAGAC GAAGAC(2/6) BbvCI 246 CCTCAGC CCTCAGC(-5/-2) BclI 2192 TGATCA T^GATCA BglI 339 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2444 AGATCT A^GATCT BlnI 2832 CCTAGG C^CTAGG BpuAI 656 GAAGAC GAAGAC(2/6) BsaAI 101 CACGTA [CT]AC^GT[GA] BsaHI 739 GACGTC G[GA]^CG[CT]C BspLU11I 1473 ACATGT A^CATGT BspMI 964 GCAGGT ACCTGC(4/8) BsrBI 940 GAGCGG CCGCTC(-3/-3) BsrFI 2916 GCCGGC [GA]^CCGG[CT] BstBI 1060 TTCGAA TT^CGAA BstUI 929 CGCG CG^CG Bsu36I 363 CCTCAGG CC^T[ACGT]AGG CfoI 928 GCGC GCG^C Cfr10I 2916 GCCGGC [GA]^CCGG[CT] DraIII 1178 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 2344 GAAGAG CTCTTC(1/4) Eco81I 363 CCTCAGG CC^T[ACGT]AGG EcoRI 1228 GAATTC G^AATTC EcoRV 195 GATATC GAT^ATC EcoT22I 1858 ATGCAT ATGCA^T FbaI 2192 TGATCA T^GATCA HgaI 1048 GACGC GACGC(5/10) HhaI 928 GCGC GCG^C Hin1I 739 GACGTC G[GA]^CG[CT]C HinP1I 928 GCGC G^CGC HpaI 2248 GTTAAC GTT^AAC Ksp632I 2344 GAAGAG CTCTTC(1/4) MvnI 929 CGCG CG^CG NaeI 2916 GCCGGC GCC^GGC NdeI 2950 CATATG CA^TATG NgoMIV 2916 GCCGGC G^CCGGC NsiI 1858 ATGCAT ATGCA^T NspV 1060 TTCGAA TT^CGAA PmeI 459 GTTTAAAC GTTT^AAAC PshBI 2474 ATTAAT AT^TAAT PstI 3065 CTGCAG CTGCA^G SalI 898 GTCGAC G^TCGAC ScaI 2881 AGTACT AGT^ACT SfiI 338 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1060 TTCGAA TT^CGAA SphI 1856 GCATGC GCATG^C SwaI 1081 ATTTAAAT ATTT^AAAT XbaI 2487 TCTAGA T^CTAGA XmnI 1593 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AspHI 1175 GAGCAC G[AT]GC[AT]^C 1524 GAGCAC AspI 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1048 GACGCTGTC BciVI 501 GTATCC GTATCC(6/5) 2795 GTATCC BcnI 222 CCCGG CC^[GC]GG 2163 CCCGG BmyI 1175 GAGCAC G[AGT]GC[ACT]^C 1524 GAGCAC BpmI 651 CTCCAG CTGGAG(16/14) 2780 CTCCAG BsgI 421 CTGCAC GTGCAG(16/14) 2042 GTGCAG BsiHKAI 1175 GAGCAC G[AT]GC[AT]^C 1524 GAGCAC BsmBI 150 GAGACG CGTCTC(1/5) 317 GAGACG Bsp1286I 1175 GAGCAC G[AGT]GC[ACT]^C 1524 GAGCAC BsrDI 646 CATTGC GCAATG(2/0) 185 GCAATG Bst1107I 727 GTATAC GTA^TAC 3072 GTATAC BstZ17I 727 GTATAC GTA^TAC 3072 GTATAC FauI 947 CCCGC CCCGC(4/6) 2219 CCCGC HindIII 38 AAGCTT A^AGCTT 3015 AAGCTT MaeII 102 ACGT A^CGT 740 ACGT NciI 222 CCCGG CC^[GC]GG 2163 CCCGG NcoI 1537 CCATGG C^CATGG 2157 CCATGG PflFI 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1048 GACGCTGTC Tth111I 152 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1048 GACGCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BalI BbvI BfaI BsaJI BseRI BsiYI BslI BsmAI BsmFI BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII