##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BbrPI BbvCI BglI
BlnI BlpI Bpu1102I BsaAI BseAI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrGI BssSI Bst1107I
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraIII EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
FseI FspI HindIII HpaI KpnI
MluI MroI NotI NruI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflII 511 CTTAAG C^TTAAG
AhdI 954 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1264 GTGCAC G^TGCAC
ApaLI 1264 GTGCAC G^TGCAC
AspEI 954 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1273 TGGCCA TGG^CCA
BamHI 3604 GGATCC G^GATCC
BciVI 2522 GGATAC GTATCC(6/5)
BclI 1972 TGATCA T^GATCA
BfrI 511 CTTAAG C^TTAAG
BsaWI 715 ACCGGA [AT]^CCGG[AT]
BsiEI 15 CGGTCG CG[GA][CT]^CG
BsmBI 3265 GAGACG CGTCTC(1/5)
BspHI 440 TCATGA T^CATGA
BsrDI 1343 CATTGC GCAATG(2/0)
BssHII 95 GCGCGC G^CGCGC
BstAPI 2886 GCAGTGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3375 TTCGAA TT^CGAA
DrdI 1466 GACAAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 954 GACTCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2932 GAATTC G^AATTC
EcoRV 2696 GATATC GAT^ATC
FbaI 1972 TGATCA T^GATCA
HincII 3517 GTCAAC GT[CT]^[GA]AC
HindII 3517 GTCAAC GT[CT]^[GA]AC
KspI 169 CCGCGG CCGC^GG
MfeI 704 CAATTG C^AATTG
MluNI 1273 TGGCCA TGG^CCA
MscI 1273 TGGCCA TGG^CCA
MunI 704 CAATTG C^AATTG
NcoI 934 CCATGG C^CATGG
NdeI 2481 CATATG CA^TATG
NspV 3375 TTCGAA TT^CGAA
PpuMI 3381 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 440 TCATGA T^CATGA
SacII 169 CCGCGG CCGC^GG
SapI 1007 GAAGAGC GCTCTTC(1/4)
ScaI 3139 AGTACT AGT^ACT
SexAI 3061 ACCAGGT A^CC[AT]GGT
SfuI 3375 TTCGAA TT^CGAA
SphI 2288 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3401 GTAGAC GT^[AC][GT]AC
3421 GTAGAC
Asp700I 1391 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1947 GAATACATTC
AvaII 931 GGTCC G^G[AT]CC
3382 GGACC
BcgI 9 GCAGCACGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2490 GCATGGTTCTCG
BcnI 112 CCGGG CC^[GC]GG
3171 CCCGG
BsaBI 2731 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3326 GATGCCAATC
BsaI 3058 GAGACC GGTCTC(1/5)
1060 GGTCTC
BsrBI 180 CCGCTC CCGCTC(-3/-3)
2402 CCGCTC
DraI 844 TTTAAA TTT^AAA
3311 TTTAAA
EaeI 1273 TGGCCA [CT]^GGCC[GA]
3179 CGGCCA
EcoT22I 1053 ATGCAT ATGCA^T
2590 ATGCAT
HgaI 3167 GACGC GACGC(5/10)
2281 GCGTC
MaeII 1148 ACGT A^CGT
1262 ACGT
MamI 2731 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3326 GATGCCAATC
NaeI 117 GCCGGC GCC^GGC
1572 GCCGGC
NciI 112 CCGGG CC^[GC]GG
3171 CCCGG
NgoMIV 117 GCCGGC G^CCGGC
1572 GCCGGC
NheI 1582 GCTAGC G^CTAGC
3439 GCTAGC
NsiI 1053 ATGCAT ATGCA^T
2590 ATGCAT
NspI 1431 ACATGC [GA]CATG^[CT]
2288 GCATGC
SacI 2447 GAGCTC GAGCT^C
3119 GAGCTC
SmlI 511 CTTAAG C^T[CT][GA]AG
2341 CTTGAG
XmnI 1391 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1947 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
BanI BanII BbeI BbsI BbvI
BfaI BglII BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII