##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AscI Asp718I AviII
BalI BbeI BbrPI BciVI BclI
BsaAI BsaHI BseAI BsiWI BspEI
BspHI BspLU11I BssHII Bst1107I BstBI
BstZ17I CpoI DraI DrdI EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MfeI MluI MluNI
MroI MscI MunI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 2806 GAATTT [GA]^AATT[CT]
AflII 1423 CTTAAG C^TTAAG
AflIII 1565 ACGTGT A^C[GA][CT]GT
Alw44I 2930 GTGCAC G^TGCAC
Aor51HI 3093 AGCGCT AGC^GCT
ApaLI 2930 GTGCAC G^TGCAC
ApoI 2806 GAATTT [GA]^AATT[CT]
AseI 2647 ATTAAT AT^TAAT
AsnI 2647 ATTAAT AT^TAAT
Asp700I 2494 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1190 GGATCC G^GATCC
BanI 2419 GGTGCC G^G[CT][GA]CC
BfrI 1423 CTTAAG C^TTAAG
BglII 2336 AGATCT A^GATCT
BsaBI 1191 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1200 CGGCCG CG[GA][CT]^CG
Bsp1407I 1680 TGTACA T^GTACA
BspDI 3343 ATCGAT AT^CGAT
BsrBI 186 CCGCTC CCGCTC(-3/-3)
BsrDI 1550 GCAATG GCAATG(2/0)
BsrFI 1172 GCCGGC [GA]^CCGG[CT]
BsrGI 1680 TGTACA T^GTACA
BssSI 1716 CACGAG CACGAG(-5/-1)
BstEII 1878 GGTGACC G^GT[ACGT]ACC
BstPI 1878 GGTGACC G^GT[ACGT]ACC
BstXI 531 CCACCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1172 GCCGGC [GA]^CCGG[CT]
ClaI 3343 ATCGAT AT^CGAT
DraIII 385 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1200 CGGCCG C^GGCCG
EclXI 1200 CGGCCG C^GGCCG
Eco47III 3093 AGCGCT AGC^GCT
Eco52I 1200 CGGCCG C^GGCCG
EcoO65I 1878 GGTGACC G^GT[ACGT]ACC
MamI 1191 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1172 GCCGGC GCC^GGC
NcoI 1925 CCATGG C^CATGG
NgoMIV 1172 GCCGGC G^CCGGC
PflFI 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 702 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2647 ATTAAT AT^TAAT
SapI 3172 GAAGAGC GCTCTTC(1/4)
SexAI 1655 ACCTGGT A^CC[AT]GGT
SspBI 1680 TGTACA T^GTACA
TfiI 1246 GATTC G^A[AT]TC
Tth111I 1654 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 702 CCAACAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2494 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 66 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1138 GACCTGCTGTC
AlwI 1191 GATCC GGATC(4/5)
1190 GGATC
AspEI 66 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1138 GACCTGCTGTC
BbsI 2728 GAAGAC GAAGAC(2/6)
2887 GAAGAC
BcgI 1563 CGACGTGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
82 GCAGTGAGCTCG
BglI 2379 GCCCGAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3325 GCCATGCTGGC
BlpI 981 GCTCAGC GC^T[ACGT]AGC
1392 GCTCAGC
Bpu1102I 981 GCTCAGC GC^T[ACGT]AGC
1392 GCTCAGC
BpuAI 2728 GAAGAC GAAGAC(2/6)
2887 GAAGAC
BsaI 73 GGTCTC GGTCTC(1/5)
1851 GGTCTC
BsaWI 168 TCCGGT [AT]^CCGG[AT]
1535 ACCGGA
BsmBI 298 CGTCTC CGTCTC(1/5)
1775 CGTCTC
BsmI 1346 GAATGC GAATGC(1/-1)
407 GCATTC
BstAPI 819 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
850 GCAGAGACTGC
BstYI 1190 GGATCC [GA]^GATC[CT]
2336 AGATCT
CelII 981 GCTCAGC GC^T[ACGT]AGC
1392 GCTCAGC
Eam1105I 66 GACCGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1138 GACCTGCTGTC
HgaI 2565 GACGC GACGC(5/10)
1055 GCGTC
MaeII 297 ACGT A^CGT
1565 ACGT
MflI 1190 GGATCC [GA]^GATC[CT]
2336 AGATCT
MseI 1424 TTAA T^TAA
2648 TTAA
SspI 1069 AATATT AAT^ATT
2762 AATATT
TaqI 147 TCGA T^CGA
3344 TCGA
Tru9I 1424 TTAA T^TAA
2648 TTAA
Tsp509I 1225 AATT ^AATT
2807 AATT
TthHB8I 147 TCGA T^CGA
3344 TCGA
XcmI 443 CCACAATGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2015 CCATGCCCTCAGTGG
XhoII 1190 GGATCC [GA]^GATC[CT]
2336 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
AvrII BanII BbvCI BbvI BcnI
BfaI BlnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TseI Tsp45I TspRI XmaCI XmaI