##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BcgI BclI BfrI
BlnI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspEI BspHI BspLU11I
BssSI Bst1107I BstBI BstZ17I DraIII
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 161 GTAGAC GT^[AC][GT]AC
Alw44I 839 GTGCAC G^TGCAC
ApaI 870 GGGCCC GGGCC^C
ApaLI 839 GTGCAC G^TGCAC
BbrPI 2624 CACGTG CAC^GTG
BbsI 1275 GTCTTC GAAGAC(2/6)
BciVI 179 GGATAC GTATCC(6/5)
BfaI 758 CTAG C^TAG
BpuAI 1275 GTCTTC GAAGAC(2/6)
BsaAI 2624 CACGTG [CT]AC^GT[GA]
BsaBI 982 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 228 TGTACA T^GTACA
BspDI 167 ATCGAT AT^CGAT
BsrGI 228 TGTACA T^GTACA
BssHII 2053 GCGCGC G^CGCGC
BstAPI 11 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2565 GGTCACC G^GT[ACGT]ACC
BstPI 2565 GGTCACC G^GT[ACGT]ACC
ClaI 167 ATCGAT AT^CGAT
CpoI 1110 CGGTCCG CG^G[AT]CCG
DraI 1713 TTTAAA TTT^AAA
EcoO65I 2565 GGTCACC G^GT[ACGT]ACC
HgaI 794 GACGC GACGC(5/10)
MaeI 758 CTAG C^TAG
MamI 982 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 441 GCCGGC GCC^GGC
NgoMIV 441 GCCGGC G^CCGGC
NruI 990 TCGCGA TCG^CGA
PaeR7I 1495 CTCGAG C^TCGAG
PmaCI 2624 CACGTG CAC^GTG
PmlI 2624 CACGTG CAC^GTG
RsrII 1110 CGGTCCG CG^G[AT]CCG
SapI 298 GAAGAGC GCTCTTC(1/4)
SmaI 1556 CCCGGG CCC^GGG
SphI 1668 GCATGC GCATG^C
SspBI 228 TGTACA T^GTACA
XhoI 1495 CTCGAG C^TCGAG
XmaCI 1556 CCCGGG C^CCGGG
XmaI 1556 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 854 GGCGCC G[GA]^CG[CT]C
2013 GGCGCC
AflIII 2396 ACGTGT A^C[GA][CT]GT
2623 ACACGT
AhdI 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2868 GACCAGGGGTC
AocI 2574 CCTGAGG CC^T[ACGT]AGG
2971 CCTGAGG
AspEI 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2868 GACCAGGGGTC
BalI 2746 TGGCCA TGG^CCA
2998 TGGCCA
BamHI 1507 GGATCC G^GATCC
1860 GGATCC
BbeI 854 GGCGCC GGCGC^C
2013 GGCGCC
BglI 1492 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2146 GCCTTCTGGGC
BglII 1467 AGATCT A^GATCT
1771 AGATCT
BsaHI 854 GGCGCC G[GA]^CG[CT]C
2013 GGCGCC
BsrBI 752 CCGCTC CCGCTC(-3/-3)
2425 CCGCTC
BsrDI 7 CATTGC GCAATG(2/0)
879 GCAATG
Bsu36I 2574 CCTGAGG CC^T[ACGT]AGG
2971 CCTGAGG
DrdI 1103 GACGTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2773 GACGGTGGAGTC
Eam1105I 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2868 GACCAGGGGTC
Eco81I 2574 CCTGAGG CC^T[ACGT]AGG
2971 CCTGAGG
EcoNI 844 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2106 CCTCTACCAGG
HaeII 854 GGCGCC [GA]GCGC^[CT]
2013 GGCGCC
Hin1I 854 GGCGCC G[GA]^CG[CT]C
2013 GGCGCC
HincII 1382 GTCAAC GT[CT]^[GA]AC
3053 GTTGAC
HindII 1382 GTCAAC GT[CT]^[GA]AC
3053 GTTGAC
HindIII 1718 AAGCTT A^AGCTT
1854 AAGCTT
KasI 854 GGCGCC G^GCGCC
2013 GGCGCC
MluNI 2746 TGGCCA TGG^CCA
2998 TGGCCA
MscI 2746 TGGCCA TGG^CCA
2998 TGGCCA
MseI 1714 TTAA T^TAA
1733 TTAA
NarI 854 GGCGCC GG^CGCC
2013 GGCGCC
NcoI 531 CCATGG C^CATGG
2069 CCATGG
SacI 234 GAGCTC GAGCT^C
1241 GAGCTC
SbfI 2040 CCTGCAGG CCTGCA^GG
2486 CCTGCAGG
SexAI 216 ACCAGGT A^CC[AT]GGT
2111 ACCAGGT
SfoI 854 GGCGCC GGC^GCC
2013 GGCGCC
SmlI 1391 CTCAAG C^T[CT][GA]AG
1495 CTCGAG
Sse8387I 2040 CCTGCAGG CCTGCA^GG
2486 CCTGCAGG
Tru9I 1714 TTAA T^TAA
1733 TTAA
Tsp509I 3039 AATT ^AATT
3101 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII