################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BcgI BclI BfrI BlnI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspHI BspLU11I BssSI Bst1107I BstBI BstZ17I DraIII EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NdeI NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI SacII SalI ScaI SfiI SfuI SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 161 GTAGAC GT^[AC][GT]AC Alw44I 839 GTGCAC G^TGCAC ApaI 870 GGGCCC GGGCC^C ApaLI 839 GTGCAC G^TGCAC BbrPI 2624 CACGTG CAC^GTG BbsI 1275 GTCTTC GAAGAC(2/6) BciVI 179 GGATAC GTATCC(6/5) BfaI 758 CTAG C^TAG BpuAI 1275 GTCTTC GAAGAC(2/6) BsaAI 2624 CACGTG [CT]AC^GT[GA] BsaBI 982 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC Bsp1407I 228 TGTACA T^GTACA BspDI 167 ATCGAT AT^CGAT BsrGI 228 TGTACA T^GTACA BssHII 2053 GCGCGC G^CGCGC BstAPI 11 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2565 GGTCACC G^GT[ACGT]ACC BstPI 2565 GGTCACC G^GT[ACGT]ACC ClaI 167 ATCGAT AT^CGAT CpoI 1110 CGGTCCG CG^G[AT]CCG DraI 1713 TTTAAA TTT^AAA EcoO65I 2565 GGTCACC G^GT[ACGT]ACC HgaI 794 GACGC GACGC(5/10) MaeI 758 CTAG C^TAG MamI 982 GATAGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 441 GCCGGC GCC^GGC NgoMIV 441 GCCGGC G^CCGGC NruI 990 TCGCGA TCG^CGA PaeR7I 1495 CTCGAG C^TCGAG PmaCI 2624 CACGTG CAC^GTG PmlI 2624 CACGTG CAC^GTG RsrII 1110 CGGTCCG CG^G[AT]CCG SapI 298 GAAGAGC GCTCTTC(1/4) SmaI 1556 CCCGGG CCC^GGG SphI 1668 GCATGC GCATG^C SspBI 228 TGTACA T^GTACA XhoI 1495 CTCGAG C^TCGAG XmaCI 1556 CCCGGG C^CCGGG XmaI 1556 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 854 GGCGCC G[GA]^CG[CT]C 2013 GGCGCC AflIII 2396 ACGTGT A^C[GA][CT]GT 2623 ACACGT AhdI 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2868 GACCAGGGGTC AocI 2574 CCTGAGG CC^T[ACGT]AGG 2971 CCTGAGG AspEI 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2868 GACCAGGGGTC BalI 2746 TGGCCA TGG^CCA 2998 TGGCCA BamHI 1507 GGATCC G^GATCC 1860 GGATCC BbeI 854 GGCGCC GGCGC^C 2013 GGCGCC BglI 1492 GCCCTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2146 GCCTTCTGGGC BglII 1467 AGATCT A^GATCT 1771 AGATCT BsaHI 854 GGCGCC G[GA]^CG[CT]C 2013 GGCGCC BsrBI 752 CCGCTC CCGCTC(-3/-3) 2425 CCGCTC BsrDI 7 CATTGC GCAATG(2/0) 879 GCAATG Bsu36I 2574 CCTGAGG CC^T[ACGT]AGG 2971 CCTGAGG DrdI 1103 GACGTGCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2773 GACGGTGGAGTC Eam1105I 2493 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2868 GACCAGGGGTC Eco81I 2574 CCTGAGG CC^T[ACGT]AGG 2971 CCTGAGG EcoNI 844 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2106 CCTCTACCAGG HaeII 854 GGCGCC [GA]GCGC^[CT] 2013 GGCGCC Hin1I 854 GGCGCC G[GA]^CG[CT]C 2013 GGCGCC HincII 1382 GTCAAC GT[CT]^[GA]AC 3053 GTTGAC HindII 1382 GTCAAC GT[CT]^[GA]AC 3053 GTTGAC HindIII 1718 AAGCTT A^AGCTT 1854 AAGCTT KasI 854 GGCGCC G^GCGCC 2013 GGCGCC MluNI 2746 TGGCCA TGG^CCA 2998 TGGCCA MscI 2746 TGGCCA TGG^CCA 2998 TGGCCA MseI 1714 TTAA T^TAA 1733 TTAA NarI 854 GGCGCC GG^CGCC 2013 GGCGCC NcoI 531 CCATGG C^CATGG 2069 CCATGG SacI 234 GAGCTC GAGCT^C 1241 GAGCTC SbfI 2040 CCTGCAGG CCTGCA^GG 2486 CCTGCAGG SexAI 216 ACCAGGT A^CC[AT]GGT 2111 ACCAGGT SfoI 854 GGCGCC GGC^GCC 2013 GGCGCC SmlI 1391 CTCAAG C^T[CT][GA]AG 1495 CTCGAG Sse8387I 2040 CCTGCAGG CCTGCA^GG 2486 CCTGCAGG Tru9I 1714 TTAA T^TAA 1733 TTAA Tsp509I 3039 AATT ^AATT 3101 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII