##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BamHI BbeI
BbrPI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstZ17I CelII Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SgrAI SnaBI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AflII 818 CTTAAG C^TTAAG
Aor51HI 39 AGCGCT AGC^GCT
ApaI 248 GGGCCC GGGCC^C
AseI 3058 ATTAAT AT^TAAT
AsnI 3058 ATTAAT AT^TAAT
AspHI 470 GAGCTC G[AT]GC[AT]^C
BalI 799 TGGCCA TGG^CCA
BfrI 818 CTTAAG C^TTAAG
BsiHKAI 470 GAGCTC G[AT]GC[AT]^C
BsmBI 2144 GAGACG CGTCTC(1/5)
BsrDI 2179 GCAATG GCAATG(2/0)
BssSI 218 CTCGTG CACGAG(-5/-1)
BstBI 1098 TTCGAA TT^CGAA
BstEII 1989 GGTGACC G^GT[ACGT]ACC
BstPI 1989 GGTGACC G^GT[ACGT]ACC
BstYI 194 AGATCC [GA]^GATC[CT]
DraII 1538 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 799 TGGCCA [CT]^GGCC[GA]
EarI 1720 CTCTTC CTCTTC(1/4)
Eco47III 39 AGCGCT AGC^GCT
EcoO109I 1538 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1989 GGTGACC G^GT[ACGT]ACC
HindIII 1690 AAGCTT A^AGCTT
Ksp632I 1720 CTCTTC CTCTTC(1/4)
MaeII 3227 ACGT A^CGT
MflI 194 AGATCC [GA]^GATC[CT]
MluNI 799 TGGCCA TGG^CCA
MscI 799 TGGCCA TGG^CCA
NheI 20 GCTAGC G^CTAGC
NspI 1276 GCATGC [GA]CATG^[CT]
NspV 1098 TTCGAA TT^CGAA
PflMI 849 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1538 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 3058 ATTAAT AT^TAAT
SacI 470 GAGCTC GAGCT^C
SfuI 1098 TTCGAA TT^CGAA
SmaI 2216 CCCGGG CCC^GGG
SphI 1276 GCATGC GCATG^C
SspI 3343 AATATT AAT^ATT
Van91I 849 CCAAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 194 AGATCC [GA]^GATC[CT]
XmaCI 2216 CCCGGG C^CCGGG
XmaI 2216 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 734 CCTCAGG CC^T[ACGT]AGG
1984 CCTCAGG
BanI 646 GGTGCC G^G[CT][GA]CC
2268 GGTGCC
BanII 248 GGGCCC G[GA]GC[CT]^C
470 GAGCTC
BbsI 213 GAAGAC GAAGAC(2/6)
3015 GAAGAC
BcgI 27 CGAGCGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2228 CGATTCTCCTGC
BpuAI 213 GAAGAC GAAGAC(2/6)
3015 GAAGAC
BsgI 1805 GTGCAG GTGCAG(16/14)
1899 GTGCAG
BspMI 1993 ACCTGC ACCTGC(4/8)
1893 GCAGGT
Bsu36I 734 CCTCAGG CC^T[ACGT]AGG
1984 CCTCAGG
DraIII 1126 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
2571 CACAAAGTG
Eco81I 734 CCTCAGG CC^T[ACGT]AGG
1984 CCTCAGG
EcoT14I 849 CCAAGG C^C[AT][AT]GG
1393 CCTTGG
HaeII 29 AGCGCC [GA]GCGC^[CT]
39 AGCGCT
MwoI 1192 GCGATCTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2187 GCGATCTCGGC
PvuII 99 CAGCTG CAG^CTG
165 CAGCTG
ScaI 117 AGTACT AGT^ACT
2764 AGTACT
SpeI 2249 ACTAGT A^CTAGT
2478 ACTAGT
StyI 849 CCAAGG C^C[AT][AT]GG
1393 CCTTGG
TaqI 722 TCGA T^CGA
1099 TCGA
TthHB8I 722 TCGA T^CGA
1099 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AvaI AvaII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI NciI NdeII NlaIII NlaIV
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XmnI