##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccIII AcsI AflIII AgeI
AocI ApoI AscI AseI AsnI
Asp700I AspI AviII BalI BbrPI
BbsI BcgI BclI BglII BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI BstBI Bsu36I
CelII Cfr10I ClaI DraI DraIII
DrdI EagI EclXI Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI FspI HpaI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI RcaI SalI
SapI SbfI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 2633 GGTACC G^GTACC
AccII 51 CGCG CG^CG
AclI 2203 AACGTT AA^CGTT
AflII 182 CTTAAG C^TTAAG
AhdI 1941 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2881 GTGCAC G^TGCAC
Aor51HI 157 AGCGCT AGC^GCT
ApaI 686 GGGCCC GGGCC^C
ApaLI 2881 GTGCAC G^TGCAC
Asp718I 2633 GGTACC G^GTACC
AspEI 1941 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1068 GGATCC G^GATCC
BbeI 233 GGCGCC GGCGC^C
BbvCI 383 CCTCAGC CCTCAGC(-5/-2)
BfrI 182 CTTAAG C^TTAAG
BsiEI 2717 CGATCG CG[GA][CT]^CG
BsmBI 2703 CGTCTC CGTCTC(1/5)
BsmI 1173 GCATTC GAATGC(1/-1)
BsrBI 2338 GAGCGG CCGCTC(-3/-3)
BstAPI 1322 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 722 GGTTACC G^GT[ACGT]ACC
BstPI 722 GGTTACC G^GT[ACGT]ACC
BstUI 51 CGCG CG^CG
CpoI 2828 CGGTCCG CG^G[AT]CCG
DsaI 50 CCGCGG C^C[GA][CT]GG
Eam1105I 1941 GACTCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2158 GAAGAG CTCTTC(1/4)
Eco47III 157 AGCGCT AGC^GCT
EcoO65I 722 GGTTACC G^GT[ACGT]ACC
HindIII 2145 AAGCTT A^AGCTT
HphI 1100 TCACC GGTGA(8/7)
KasI 233 GGCGCC G^GCGCC
KpnI 2633 GGTACC GGTAC^C
Ksp632I 2158 GAAGAG CTCTTC(1/4)
KspI 50 CCGCGG CCGC^GG
MaeII 2204 ACGT A^CGT
MslI 2475 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 51 CGCG CG^CG
NarI 233 GGCGCC GG^CGCC
NspI 3018 ACATGC [GA]CATG^[CT]
Psp1406I 2203 AACGTT AA^CGTT
PvuI 2717 CGATCG CGAT^CG
RsrII 2828 CGGTCCG CG^G[AT]CCG
SacII 50 CCGCGG CCGC^GG
ScaI 3024 AGTACT AGT^ACT
SexAI 2896 ACCTGGT A^CC[AT]GGT
SfoI 233 GGCGCC GGC^GCC
SmlI 182 CTTAAG C^T[CT][GA]AG
SspI 3126 AATATT AAT^ATT
Tsp45I 1099 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 233 GGCGCC G[GA]^CG[CT]C
1667 GGCGTC
BciVI 610 GTATCC GTATCC(6/5)
1233 GTATCC
BsaHI 233 GGCGCC G[GA]^CG[CT]C
1667 GGCGTC
BspMI 368 GCAGGT ACCTGC(4/8)
1247 GCAGGT
BstXI 2623 CCATCCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2890 CCATCAACCTGG
BstYI 1068 GGATCC [GA]^GATC[CT]
2310 AGATCC
HgaI 287 GACGC GACGC(5/10)
1668 GCGTC
Hin1I 233 GGCGCC G[GA]^CG[CT]C
1667 GGCGTC
HincII 2851 GTTGAC GT[CT]^[GA]AC
2901 GTTGAC
HindII 2851 GTTGAC GT[CT]^[GA]AC
2901 GTTGAC
MboII 2158 GAAGA GAAGA(8/7)
2491 TCTTC
MflI 1068 GGATCC [GA]^GATC[CT]
2310 AGATCC
MseI 183 TTAA T^TAA
519 TTAA
SacI 465 GAGCTC GAGCT^C
2428 GAGCTC
Tru9I 183 TTAA T^TAA
519 TTAA
Tsp509I 859 AATT ^AATT
868 AATT
XhoII 1068 GGATCC [GA]^GATC[CT]
2310 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII AvrII
BanI BanII BbvI BcnI BfaI
BglI BlnI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI Bst1107I BstF5I BstNI BstZ17I
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeI MaeIII MboI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TfiI TseI TspRI Van91I
XcmI XmaCI XmaI