##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AviII
BbeI BbrPI BfrI BglI BlpI
Bpu1102I BsaAI BsaHI BsaI BsgI
BsiWI BsmI BspDI BspHI BsrFI
BssHII Bst1107I BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DsaI
EagI Eam1105I EclXI Eco52I EcoT22I
FseI FspI Hin1I HpaI KasI
KspI MluI MvnI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
RcaI RsrII SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 348 GGTACC G^GTACC
AccIII 139 TCCGGA T^CCGGA
AocI 851 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1228 AGCGCT AGC^GCT
Asp700I 3470 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 348 GGTACC G^GTACC
AspI 3133 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3532 CCCGGG C^[CT]CG[GA]G
AvrII 2442 CCTAGG C^CTAGG
BamHI 3528 GGATCC G^GATCC
BbvCI 1337 GCTGAGG CCTCAGC(-5/-2)
BciVI 1713 GGATAC GTATCC(6/5)
BclI 3293 TGATCA T^GATCA
BlnI 2442 CCTAGG C^CTAGG
BsaBI 1079 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 139 TCCGGA T^CCGGA
BsiEI 1066 CGATCG CG[GA][CT]^CG
BsmBI 1332 GAGACG CGTCTC(1/5)
BsoBI 3532 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2210 TGTACA T^GTACA
BspEI 139 TCCGGA T^CCGGA
BsrBI 576 GAGCGG CCGCTC(-3/-3)
BsrGI 2210 TGTACA T^GTACA
BssSI 2810 CACGAG CACGAG(-5/-1)
BstEII 1599 GGTGACC G^GT[ACGT]ACC
BstPI 1599 GGTGACC G^GT[ACGT]ACC
BstXI 2004 CCAGGGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 851 CCTCAGG CC^T[ACGT]AGG
DraII 1374 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 1698 GACAGCTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1228 AGCGCT AGC^GCT
Eco81I 851 CCTCAGG CC^T[ACGT]AGG
EcoO109I 1374 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1599 GGTGACC G^GT[ACGT]ACC
EcoRI 2698 GAATTC G^AATTC
EcoRV 2724 GATATC GAT^ATC
FbaI 3293 TGATCA T^GATCA
HaeII 1228 AGCGCT [GA]GCGC^[CT]
HincII 3050 GTCAAC GT[CT]^[GA]AC
HindII 3050 GTCAAC GT[CT]^[GA]AC
KpnI 348 GGTACC GGTAC^C
MaeII 1748 ACGT A^CGT
MamI 1079 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 139 TCCGGA T^CCGGA
NheI 1025 GCTAGC G^CTAGC
PflFI 3133 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1374 AGGACCC [GA]G^G[AT]CC[CT]
PvuI 1066 CGATCG CGAT^CG
SapI 3058 GAAGAGC GCTCTTC(1/4)
SexAI 310 ACCTGGT A^CC[AT]GGT
SmaI 3532 CCCGGG CCC^GGG
SphI 1039 GCATGC GCATG^C
SspBI 2210 TGTACA T^GTACA
Tth111I 3133 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2720 TCTAGA T^CTAGA
XcmI 2050 CCAAGCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 3532 CCCGGG C^CCGGG
XmaI 3532 CCCGGG C^CCGGG
XmnI 3470 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 2217 ACATGT A^C[GA][CT]GT
3571 ACATGT
AspHI 1635 GAGCTC G[AT]GC[AT]^C
2934 GAGCTC
BanI 250 GGTGCC G^G[CT][GA]CC
348 GGTACC
BcgI 1522 CGACTGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1397 GCATCATGGTCG
BglII 884 AGATCT A^GATCT
1826 AGATCT
BsaWI 139 TCCGGA [AT]^CCGG[AT]
1603 ACCGGA
BsiHKAI 1635 GAGCTC G[AT]GC[AT]^C
2934 GAGCTC
BspLU11I 2217 ACATGT A^CATGT
3571 ACATGT
BstAPI 361 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1985 GCAGAAGATGC
DraIII 309 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
999 CACCTAGTG
EcoNI 1427 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1590 CCTGTTGGAGG
FauI 1273 CCCGC CCCGC(4/6)
1304 GCGGG
HgaI 1334 GACGC GACGC(5/10)
3003 GCGTC
MfeI 3077 CAATTG C^AATTG
3503 CAATTG
MunI 3077 CAATTG C^AATTG
3503 CAATTG
NdeI 1240 CATATG CA^TATG
1836 CATATG
PflMI 1166 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCATCAGCTGG
SacI 1635 GAGCTC GAGCT^C
2934 GAGCTC
SmlI 760 CTCAAG C^T[CT][GA]AG
2287 CTCAAG
StuI 855 AGGCCT AGG^CCT
950 AGGCCT
TaqI 1959 TCGA T^CGA
2948 TCGA
TthHB8I 1959 TCGA T^CGA
2948 TCGA
Van91I 1166 CCAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCATCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BalI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII