##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AviII
BamHI BbeI BbrPI BcgI BclI
BfrI BglII BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HpaI KasI MamI
MfeI MluI MroI MunI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1061 GTAGAC GT^[AC][GT]AC
AcsI 2775 GAATTC [GA]^AATT[CT]
AflIII 2290 ACATGT A^C[GA][CT]GT
AocI 900 CCTCAGG CC^T[ACGT]AGG
ApoI 2775 GAATTC [GA]^AATT[CT]
AspI 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2199 CCCGAG C^[CT]CG[GA]G
AvrII 754 CCTAGG C^CTAGG
BbsI 2220 GTCTTC GAAGAC(2/6)
BlnI 754 CCTAGG C^CTAGG
BpuAI 2220 GTCTTC GAAGAC(2/6)
BsoBI 2199 CCCGAG C^[CT]CG[GA]G
BspLU11I 2290 ACATGT A^CATGT
BssHII 192 GCGCGC G^CGCGC
Bsu36I 900 CCTCAGG CC^T[ACGT]AGG
DraIII 2073 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 900 CCTCAGG CC^T[ACGT]AGG
EcoRI 2775 GAATTC G^AATTC
HincII 2304 GTTGAC GT[CT]^[GA]AC
HindII 2304 GTTGAC GT[CT]^[GA]AC
HindIII 3004 AAGCTT A^AGCTT
KspI 562 CCGCGG CCGC^GG
MaeII 2676 ACGT A^CGT
MseI 2561 TTAA T^TAA
NaeI 2853 GCCGGC GCC^GGC
NcoI 2215 CCATGG C^CATGG
NgoMIV 2853 GCCGGC G^CCGGC
NheI 2908 GCTAGC G^CTAGC
PflFI 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1718 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 353 GAGCTC GAGCT^C
SacII 562 CCGCGG CCGC^GG
SfiI 2843 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1277 CTTGAG C^T[CT][GA]AG
SspI 3277 AATATT AAT^ATT
TfiI 2141 GATTC G^A[AT]TC
Tru9I 2561 TTAA T^TAA
Tsp509I 2776 AATT ^AATT
Tth111I 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 701 GGTACC G^GTACC
2985 GGTACC
AhdI 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACCATGTGTC
Asp718I 701 GGTACC G^GTACC
2985 GGTACC
AspEI 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACCATGTGTC
AspHI 56 GTGCTC G[AT]GC[AT]^C
353 GAGCTC
BalI 1850 TGGCCA TGG^CCA
2999 TGGCCA
BbvCI 3070 CCTCAGC CCTCAGC(-5/-2)
2262 GCTGAGG
BciVI 1640 GTATCC GTATCC(6/5)
1740 GTATCC
BglI 2612 GCCTATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2844 GCCTCCCAGGC
BsiHKAI 56 GTGCTC G[AT]GC[AT]^C
353 GAGCTC
BspMI 830 ACCTGC ACCTGC(4/8)
2237 GCAGGT
BsrBI 1930 GAGCGG CCGCTC(-3/-3)
2897 GAGCGG
BsrFI 2186 GCCGGT [GA]^CCGG[CT]
2853 GCCGGC
BstYI 969 AGATCC [GA]^GATC[CT]
2402 AGATCC
Cfr10I 2186 GCCGGT [GA]^CCGG[CT]
2853 GCCGGC
DrdI 638 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2268 GACTATGGGGTC
Eam1105I 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1549 GACCATGTGTC
HaeII 1991 GGCGCT [GA]GCGC^[CT]
2050 GGCGCT
KpnI 701 GGTACC GGTAC^C
2985 GGTACC
MflI 969 AGATCC [GA]^GATC[CT]
2402 AGATCC
MluNI 1850 TGGCCA TGG^CCA
2999 TGGCCA
MscI 1850 TGGCCA TGG^CCA
2999 TGGCCA
NspI 2290 ACATGT [GA]CATG^[CT]
2807 GCATGT
SexAI 1065 ACCAGGT A^CC[AT]GGT
1635 ACCTGGT
XbaI 1408 TCTAGA T^CTAGA
1620 TCTAGA
XhoII 969 AGATCC [GA]^GATC[CT]
2402 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I