################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AviII BamHI BbeI BbrPI BcgI BclI BfrI BglII BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI FspI Hin1I HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 1061 GTAGAC GT^[AC][GT]AC AcsI 2775 GAATTC [GA]^AATT[CT] AflIII 2290 ACATGT A^C[GA][CT]GT AocI 900 CCTCAGG CC^T[ACGT]AGG ApoI 2775 GAATTC [GA]^AATT[CT] AspI 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2199 CCCGAG C^[CT]CG[GA]G AvrII 754 CCTAGG C^CTAGG BbsI 2220 GTCTTC GAAGAC(2/6) BlnI 754 CCTAGG C^CTAGG BpuAI 2220 GTCTTC GAAGAC(2/6) BsoBI 2199 CCCGAG C^[CT]CG[GA]G BspLU11I 2290 ACATGT A^CATGT BssHII 192 GCGCGC G^CGCGC Bsu36I 900 CCTCAGG CC^T[ACGT]AGG DraIII 2073 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 900 CCTCAGG CC^T[ACGT]AGG EcoRI 2775 GAATTC G^AATTC HincII 2304 GTTGAC GT[CT]^[GA]AC HindII 2304 GTTGAC GT[CT]^[GA]AC HindIII 3004 AAGCTT A^AGCTT KspI 562 CCGCGG CCGC^GG MaeII 2676 ACGT A^CGT MseI 2561 TTAA T^TAA NaeI 2853 GCCGGC GCC^GGC NcoI 2215 CCATGG C^CATGG NgoMIV 2853 GCCGGC G^CCGGC NheI 2908 GCTAGC G^CTAGC PflFI 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1718 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 353 GAGCTC GAGCT^C SacII 562 CCGCGG CCGC^GG SfiI 2843 GGCCTCCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1277 CTTGAG C^T[CT][GA]AG SspI 3277 AATATT AAT^ATT TfiI 2141 GATTC G^A[AT]TC Tru9I 2561 TTAA T^TAA Tsp509I 2776 AATT ^AATT Tth111I 394 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 701 GGTACC G^GTACC 2985 GGTACC AhdI 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1549 GACCATGTGTC Asp718I 701 GGTACC G^GTACC 2985 GGTACC AspEI 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1549 GACCATGTGTC AspHI 56 GTGCTC G[AT]GC[AT]^C 353 GAGCTC BalI 1850 TGGCCA TGG^CCA 2999 TGGCCA BbvCI 3070 CCTCAGC CCTCAGC(-5/-2) 2262 GCTGAGG BciVI 1640 GTATCC GTATCC(6/5) 1740 GTATCC BglI 2612 GCCTATGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2844 GCCTCCCAGGC BsiHKAI 56 GTGCTC G[AT]GC[AT]^C 353 GAGCTC BspMI 830 ACCTGC ACCTGC(4/8) 2237 GCAGGT BsrBI 1930 GAGCGG CCGCTC(-3/-3) 2897 GAGCGG BsrFI 2186 GCCGGT [GA]^CCGG[CT] 2853 GCCGGC BstYI 969 AGATCC [GA]^GATC[CT] 2402 AGATCC Cfr10I 2186 GCCGGT [GA]^CCGG[CT] 2853 GCCGGC DrdI 638 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2268 GACTATGGGGTC Eam1105I 1064 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1549 GACCATGTGTC HaeII 1991 GGCGCT [GA]GCGC^[CT] 2050 GGCGCT KpnI 701 GGTACC GGTAC^C 2985 GGTACC MflI 969 AGATCC [GA]^GATC[CT] 2402 AGATCC MluNI 1850 TGGCCA TGG^CCA 2999 TGGCCA MscI 1850 TGGCCA TGG^CCA 2999 TGGCCA NspI 2290 ACATGT [GA]CATG^[CT] 2807 GCATGT SexAI 1065 ACCAGGT A^CC[AT]GGT 1635 ACCTGGT XbaI 1408 TCTAGA T^CTAGA 1620 TCTAGA XhoII 969 AGATCC [GA]^GATC[CT] 2402 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I