##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AviII AvrII BalI BbeI BbvCI
BcgI BciVI BglI BlnI BlpI
Bpu1102I BsiHKAI BsiWI BspDI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
FseI FspI HaeII HpaI KasI
KpnI MluI MluNI MscI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SalI SfiI SfoI SfuI SgrAI
SnaBI SpeI StuI SwaI Van91I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 1011 GACGTC GACGT^C
AflII 2937 CTTAAG C^TTAAG
ApaI 1377 GGGCCC GGGCC^C
AvaI 985 CCCGGG C^[CT]CG[GA]G
BbrPI 3205 CACGTG CAC^GTG
BbsI 1050 GTCTTC GAAGAC(2/6)
BclI 2498 TGATCA T^GATCA
BfrI 2937 CTTAAG C^TTAAG
BpuAI 1050 GTCTTC GAAGAC(2/6)
BsaAI 3205 CACGTG [CT]AC^GT[GA]
BsaBI 1476 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 977 CTGCAC GTGCAG(16/14)
BsiEI 768 CGGCCG CG[GA][CT]^CG
BsmI 2048 GCATTC GAATGC(1/-1)
BsoBI 985 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2485 TGTACA T^GTACA
BsrDI 1865 GCAATG GCAATG(2/0)
BsrFI 860 GCCGGC [GA]^CCGG[CT]
BsrGI 2485 TGTACA T^GTACA
BssHII 832 GCGCGC G^CGCGC
BstAPI 1925 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 860 GCCGGC [GA]^CCGG[CT]
DraIII 2564 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 768 CGGCCG C^GGCCG
EclXI 768 CGGCCG C^GGCCG
Eco52I 768 CGGCCG C^GGCCG
EcoNI 1461 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2046 ATGCAT ATGCA^T
FbaI 2498 TGATCA T^GATCA
MamI 1476 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3119 CAATTG C^AATTG
MunI 3119 CAATTG C^AATTG
NaeI 860 GCCGGC GCC^GGC
NcoI 1771 CCATGG C^CATGG
NgoMIV 860 GCCGGC G^CCGGC
NsiI 2046 ATGCAT ATGCA^T
PmaCI 3205 CACGTG CAC^GTG
PmlI 3205 CACGTG CAC^GTG
PstI 1819 CTGCAG CTGCA^G
SbfI 1818 CCTGCAGG CCTGCA^GG
ScaI 1133 AGTACT AGT^ACT
SexAI 1460 ACCTGGT A^CC[AT]GGT
SmaI 985 CCCGGG CCC^GGG
SphI 1930 GCATGC GCATG^C
Sse8387I 1818 CCTGCAGG CCTGCA^GG
SspBI 2485 TGTACA T^GTACA
SspI 3334 AATATT AAT^ATT
XbaI 510 TCTAGA T^CTAGA
XcmI 652 CCAGGAGTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 985 CCCGGG C^CCGGG
XmaI 985 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 621 TCCGGA T^CCGGA
1484 TCCGGA
AcyI 888 GACGCC G[GA]^CG[CT]C
1011 GACGTC
AspI 1233 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2123 GACTATGTC
BamHI 873 GGATCC G^GATCC
1810 GGATCC
BanI 922 GGCACC G^G[CT][GA]CC
1368 GGCACC
BanII 1377 GGGCCC G[GA]GC[CT]^C
2138 GAGCCC
BglII 2793 AGATCT A^GATCT
3154 AGATCT
BsaHI 888 GACGCC G[GA]^CG[CT]C
1011 GACGTC
BsaI 751 GAGACC GGTCTC(1/5)
1333 GAGACC
BsaWI 621 TCCGGA [AT]^CCGG[AT]
1484 TCCGGA
BseAI 621 TCCGGA T^CCGGA
1484 TCCGGA
BsmBI 1009 GAGACG CGTCTC(1/5)
1252 GAGACG
BspEI 621 TCCGGA T^CCGGA
1484 TCCGGA
BspLU11I 242 ACATGT A^CATGT
1876 ACATGT
DrdI 1041 GACATCGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2398 GACAATTCTGTC
EaeI 768 CGGCCG [CT]^GGCC[GA]
862 CGGCCA
EcoRI 2611 GAATTC G^AATTC
3187 GAATTC
HgaI 888 GACGC GACGC(5/10)
909 GACGC
Hin1I 888 GACGCC G[GA]^CG[CT]C
1011 GACGTC
HincII 882 GTCAAC GT[CT]^[GA]AC
2279 GTTGAC
HindII 882 GTCAAC GT[CT]^[GA]AC
2279 GTTGAC
HindIII 3019 AAGCTT A^AGCTT
3242 AAGCTT
KspI 30 CCGCGG CCGC^GG
765 CCGCGG
MroI 621 TCCGGA T^CCGGA
1484 TCCGGA
PflFI 1233 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2123 GACTATGTC
PpuMI 1823 AGGACCT [GA]G^G[AT]CC[CT]
2099 AGGACCC
PvuII 200 CAGCTG CAG^CTG
2305 CAGCTG
SacII 30 CCGCGG CCGC^GG
765 CCGCGG
SapI 1593 GAAGAGC GCTCTTC(1/4)
1559 GCTCTTC
SmlI 2218 CTCAAG C^T[CT][GA]AG
2937 CTTAAG
TfiI 133 GATTC G^A[AT]TC
594 GAATC
Tth111I 1233 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2123 GACTATGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AseI AsnI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PshBI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII