##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
AocI Aor51HI AscI AspEI AspI
AvrII BbeI BfrI BlnI BlpI
Bpu1102I BseAI BsgI BsiWI BspEI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FseI HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NspV PacI PflFI
PinAI PmeI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SnaBI Sse8387I SspI StuI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1841 GACGTC GACGT^C
Acc65I 175 GGTACC G^GTACC
AccI 344 GTCTAC GT^[AC][GT]AC
Alw44I 3047 GTGCAC G^TGCAC
ApaI 20 GGGCCC GGGCC^C
ApaLI 3047 GTGCAC G^TGCAC
Asp700I 2711 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 175 GGTACC G^GTACC
AviII 1662 TGCGCA TGC^GCA
BbrPI 1583 CACGTG CAC^GTG
BciVI 3012 GGATAC GTATCC(6/5)
BclI 2883 TGATCA T^GATCA
BglI 152 GCCTGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2112 AGATCT A^GATCT
BsaI 2642 GGTCTC GGTCTC(1/5)
BsiEI 2056 CGACCG CG[GA][CT]^CG
BsmI 1981 GCATTC GAATGC(1/-1)
BspDI 2816 ATCGAT AT^CGAT
BspHI 1344 TCATGA T^CATGA
BspMI 299 ACCTGC ACCTGC(4/8)
ClaI 2816 ATCGAT AT^CGAT
DraI 2897 TTTAAA TTT^AAA
EcoT22I 2891 ATGCAT ATGCA^T
FbaI 2883 TGATCA T^GATCA
FspI 1662 TGCGCA TGC^GCA
HindIII 3073 AAGCTT A^AGCTT
KpnI 175 GGTACC GGTAC^C
NdeI 2802 CATATG CA^TATG
NruI 114 TCGCGA TCG^CGA
NsiI 2891 ATGCAT ATGCA^T
PaeR7I 1253 CTCGAG C^TCGAG
PflMI 2078 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1583 CACGTG CAC^GTG
PmlI 1583 CACGTG CAC^GTG
RcaI 1344 TCATGA T^CATGA
SacI 1931 GAGCTC GAGCT^C
ScaI 1122 AGTACT AGT^ACT
SexAI 2077 ACCAGGT A^CC[AT]GGT
SpeI 2701 ACTAGT A^CTAGT
SphI 3462 GCATGC GCATG^C
Van91I 2078 CCAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 954 CCACAGAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1253 CTCGAG C^TCGAG
XmnI 2711 GAATAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 1841 GACGTC G[GA]^CG[CT]C
2333 GACGCC
AflIII 1584 ACGTGT A^C[GA][CT]GT
3018 ACACGT
AseI 2597 ATTAAT AT^TAAT
2659 ATTAAT
AsnI 2597 ATTAAT AT^TAAT
2659 ATTAAT
AvaII 291 GGACC G^G[AT]CC
1698 GGTCC
BalI 1491 TGGCCA TGG^CCA
1848 TGGCCA
BamHI 71 GGATCC G^GATCC
1672 GGATCC
BanI 98 GGTGCC G^G[CT][GA]CC
175 GGTACC
BanII 20 GGGCCC G[GA]GC[CT]^C
1931 GAGCTC
BbvCI 821 GCTGAGG CCTCAGC(-5/-2)
890 GCTGAGG
BcgI 980 CGAGAGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
87 GCAGCGCACTCG
BfaI 2428 CTAG C^TAG
2702 CTAG
BsaAI 1583 CACGTG [CT]AC^GT[GA]
3092 CACGTA
BsaBI 275 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2263 GATCGTCATC
BsaHI 1841 GACGTC G[GA]^CG[CT]C
2333 GACGCC
BsaWI 1098 ACCGGA [AT]^CCGG[AT]
1695 TCCGGT
BsmBI 29 GAGACG CGTCTC(1/5)
1004 GAGACG
Bsp1407I 2966 TGTACA T^GTACA
3226 TGTACA
BsrBI 1551 CCGCTC CCGCTC(-3/-3)
1686 CCGCTC
BsrGI 2966 TGTACA T^GTACA
3226 TGTACA
BstAPI 92 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
423 GCAAGCAGTGC
DraII 19 GGGGCCC [GA]G^G[ACGT]CC[CT]
20 GGGCCCC
DraIII 93 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
446 CACTTTGTG
DrdI 731 GACTCGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2333 GACGCCGCTGTC
DsaI 1926 CCGTGG C^C[GA][CT]GG
1976 CCGTGG
EcoO109I 19 GGGGCCC [GA]G^G[ACGT]CC[CT]
20 GGGCCCC
EcoRI 2138 GAATTC G^AATTC
3194 GAATTC
HgaI 63 GACGC GACGC(5/10)
2333 GACGC
Hin1I 1841 GACGTC G[GA]^CG[CT]C
2333 GACGCC
MaeI 2428 CTAG C^TAG
2702 CTAG
MamI 275 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2263 GATCGTCATC
MluNI 1491 TGGCCA TGG^CCA
1848 TGGCCA
MscI 1491 TGGCCA TGG^CCA
1848 TGGCCA
PshBI 2597 ATTAAT AT^TAAT
2659 ATTAAT
SmaI 24 CCCGGG CCC^GGG
3297 CCCGGG
SspBI 2966 TGTACA T^GTACA
3226 TGTACA
XmaCI 24 CCCGGG C^CCGGG
3297 CCCGGG
XmaI 24 CCCGGG C^CCGGG
3297 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII