##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbvCI BclI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiWI BspDI BspEI
BsrFI BssHII BstAPI BstEII BstPI
Bsu36I CelII Cfr10I ClaI CpoI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I KasI
MluI MluNI MroI MscI NaeI
NarI NcoI NgoMIV NheI NotI
NsiI PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SmaI SpeI SphI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 338 GGTACC G^GTACC
AflII 2684 CTTAAG C^TTAAG
ApaI 882 GGGCCC GGGCC^C
Asp718I 338 GGTACC G^GTACC
BamHI 2469 GGATCC G^GATCC
BbsI 298 GAAGAC GAAGAC(2/6)
BbvI 22 GCTGC GCAGC(8/12)
BcgI 563 GCACTGGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 686 GGATAC GTATCC(6/5)
BcnI 866 CCGGG CC^[GC]GG
BfrI 2684 CTTAAG C^TTAAG
BglI 383 GCCGAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 298 GAAGAC GAAGAC(2/6)
BsiEI 369 CGGCCG CG[GA][CT]^CG
BspHI 642 TCATGA T^CATGA
BspLU11I 1836 ACATGT A^CATGT
BsrBI 2551 GAGCGG CCGCTC(-3/-3)
BssSI 2785 CACGAG CACGAG(-5/-1)
Bst1107I 1077 GTATAC GTA^TAC
BstBI 611 TTCGAA TT^CGAA
BstZ17I 1077 GTATAC GTA^TAC
EaeI 369 CGGCCG [CT]^GGCC[GA]
EagI 369 CGGCCG C^GGCCG
EclXI 369 CGGCCG C^GGCCG
Eco52I 369 CGGCCG C^GGCCG
HaeII 214 GGCGCT [GA]GCGC^[CT]
HapII 866 CCGG C^CGG
HincII 1262 GTTAAC GT[CT]^[GA]AC
HindII 1262 GTTAAC GT[CT]^[GA]AC
HindIII 808 AAGCTT A^AGCTT
HpaI 1262 GTTAAC GTT^AAC
HpaII 866 CCGG C^CGG
KpnI 338 GGTACC GGTAC^C
MspI 866 CCGG C^CGG
NciI 866 CCGGG CC^[GC]GG
NdeI 2057 CATATG CA^TATG
NruI 315 TCGCGA TCG^CGA
NspV 611 TTCGAA TT^CGAA
PaeR7I 70 CTCGAG C^TCGAG
RcaI 642 TCATGA T^CATGA
SacI 413 GAGCTC GAGCT^C
SfcI 1400 CTGTAG C^T[GA][CT]AG
SfuI 611 TTCGAA TT^CGAA
SnaBI 2156 TACGTA TAC^GTA
SspI 2040 AATATT AAT^ATT
StuI 61 AGGCCT AGG^CCT
TseI 22 GCTGC G^C[AT]GC
XcmI 484 CCAAGTGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 70 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 1077 GTATAC GT^[AC][GT]AC
1402 GTAGAC
AspHI 413 GAGCTC G[AT]GC[AT]^C
1453 GAGCAC
AvaI 70 CTCGAG C^[CT]CG[GA]G
419 CCCGAG
BanI 16 GGTGCC G^G[CT][GA]CC
338 GGTACC
BfaI 46 CTAG C^TAG
1998 CTAG
BseRI 2415 CTCCTC GAGGAG(10/8)
1494 GAGGAG
BsiHKAI 413 GAGCTC G[AT]GC[AT]^C
1453 GAGCAC
BsmBI 9 GAGACG CGTCTC(1/5)
290 GAGACG
BsmFI 252 GGGAC GGGAC(10/14)
1758 GGGAC
BsoBI 70 CTCGAG C^[CT]CG[GA]G
419 CCCGAG
Bsp1407I 1833 TGTACA T^GTACA
2193 TGTACA
BspMI 1821 ACCTGC ACCTGC(4/8)
526 GCAGGT
BsrDI 1519 GCAATG GCAATG(2/0)
2135 GCAATG
BsrGI 1833 TGTACA T^GTACA
2193 TGTACA
BstXI 752 CCAGATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1976 CCAATGTATTGG
Cac8I 25 GCGGGC GC[ACGT]^[ACGT]GC
29 GCTCGC
DraII 281 AGGACCC [GA]G^G[ACGT]CC[CT]
2739 AGGACCT
EarI 1274 CTCTTC CTCTTC(1/4)
1414 GAAGAG
EcoO109I 281 AGGACCC [GA]G^G[ACGT]CC[CT]
2739 AGGACCT
HgaI 292 GACGC GACGC(5/10)
301 GACGC
Ksp632I 1274 CTCTTC CTCTTC(1/4)
1414 GAAGAG
KspI 3 CCGCGG CCGC^GG
246 CCGCGG
MaeI 46 CTAG C^TAG
1998 CTAG
PflMI 894 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2474 CCAATCCTTGG
PpuMI 281 AGGACCC [GA]G^G[AT]CC[CT]
2739 AGGACCT
SacII 3 CCGCGG CCGC^GG
246 CCGCGG
SspBI 1833 TGTACA T^GTACA
2193 TGTACA
Van91I 894 CCAAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2474 CCAATCCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaII BanII
BglII BmyI BpmI BsaAI BsaBI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI CfoI Cfr13I DdeI
DpnII DraI DsaI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeII
MaeIII MamI MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XhoII