##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
AspEI       AspI        AviII       AvrII       BalI        
BbeI        BbrPI       BbvCI       BclI        BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiWI       BspDI       BspEI       
BsrFI       BssHII      BstAPI      BstEII      BstPI       
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        Hin1I       KasI        
MluI        MluNI       MroI        MscI        NaeI        
NarI        NcoI        NgoMIV      NheI        NotI        
NsiI        PacI        PflFI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    SwaI        Tth111I     XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        338  GGTACC               G^GTACC
AflII        2684  CTTAAG               C^TTAAG
ApaI          882  GGGCCC               GGGCC^C
Asp718I       338  GGTACC               G^GTACC
BamHI        2469  GGATCC               G^GATCC
BbsI          298  GAAGAC               GAAGAC(2/6)
BbvI           22  GCTGC                GCAGC(8/12)
BcgI          563  GCACTGGTGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         686  GGATAC               GTATCC(6/5)
BcnI          866  CCGGG                CC^[GC]GG
BfrI         2684  CTTAAG               C^TTAAG
BglI          383  GCCGAGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         298  GAAGAC               GAAGAC(2/6)
BsiEI         369  CGGCCG               CG[GA][CT]^CG
BspHI         642  TCATGA               T^CATGA
BspLU11I     1836  ACATGT               A^CATGT
BsrBI        2551  GAGCGG               CCGCTC(-3/-3)
BssSI        2785  CACGAG               CACGAG(-5/-1)
Bst1107I     1077  GTATAC               GTA^TAC
BstBI         611  TTCGAA               TT^CGAA
BstZ17I      1077  GTATAC               GTA^TAC
EaeI          369  CGGCCG               [CT]^GGCC[GA]
EagI          369  CGGCCG               C^GGCCG
EclXI         369  CGGCCG               C^GGCCG
Eco52I        369  CGGCCG               C^GGCCG
HaeII         214  GGCGCT               [GA]GCGC^[CT]
HapII         866  CCGG                 C^CGG
HincII       1262  GTTAAC               GT[CT]^[GA]AC
HindII       1262  GTTAAC               GT[CT]^[GA]AC
HindIII       808  AAGCTT               A^AGCTT
HpaI         1262  GTTAAC               GTT^AAC
HpaII         866  CCGG                 C^CGG
KpnI          338  GGTACC               GGTAC^C
MspI          866  CCGG                 C^CGG
NciI          866  CCGGG                CC^[GC]GG
NdeI         2057  CATATG               CA^TATG
NruI          315  TCGCGA               TCG^CGA
NspV          611  TTCGAA               TT^CGAA
PaeR7I         70  CTCGAG               C^TCGAG
RcaI          642  TCATGA               T^CATGA
SacI          413  GAGCTC               GAGCT^C
SfcI         1400  CTGTAG               C^T[GA][CT]AG
SfuI          611  TTCGAA               TT^CGAA
SnaBI        2156  TACGTA               TAC^GTA
SspI         2040  AATATT               AAT^ATT
StuI           61  AGGCCT               AGG^CCT
TseI           22  GCTGC                G^C[AT]GC
XcmI          484  CCAAGTGGAAGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI           70  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         1077  GTATAC               GT^[AC][GT]AC
             1402  GTAGAC              
AspHI         413  GAGCTC               G[AT]GC[AT]^C
             1453  GAGCAC              
AvaI           70  CTCGAG               C^[CT]CG[GA]G
              419  CCCGAG              
BanI           16  GGTGCC               G^G[CT][GA]CC
              338  GGTACC              
BfaI           46  CTAG                 C^TAG
             1998  CTAG                
BseRI        2415  CTCCTC               GAGGAG(10/8)
             1494  GAGGAG              
BsiHKAI       413  GAGCTC               G[AT]GC[AT]^C
             1453  GAGCAC              
BsmBI           9  GAGACG               CGTCTC(1/5)
              290  GAGACG              
BsmFI         252  GGGAC                GGGAC(10/14)
             1758  GGGAC               
BsoBI          70  CTCGAG               C^[CT]CG[GA]G
              419  CCCGAG              
Bsp1407I     1833  TGTACA               T^GTACA
             2193  TGTACA              
BspMI        1821  ACCTGC               ACCTGC(4/8)
              526  GCAGGT              
BsrDI        1519  GCAATG               GCAATG(2/0)
             2135  GCAATG              
BsrGI        1833  TGTACA               T^GTACA
             2193  TGTACA              
BstXI         752  CCAGATTTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1976  CCAATGTATTGG        
Cac8I          25  GCGGGC               GC[ACGT]^[ACGT]GC
               29  GCTCGC              
DraII         281  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2739  AGGACCT             
EarI         1274  CTCTTC               CTCTTC(1/4)
             1414  GAAGAG              
EcoO109I      281  AGGACCC              [GA]G^G[ACGT]CC[CT]
             2739  AGGACCT             
HgaI          292  GACGC                GACGC(5/10)
              301  GACGC               
Ksp632I      1274  CTCTTC               CTCTTC(1/4)
             1414  GAAGAG              
KspI            3  CCGCGG               CCGC^GG
              246  CCGCGG              
MaeI           46  CTAG                 C^TAG
             1998  CTAG                
PflMI         894  CCAAGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2474  CCAATCCTTGG         
PpuMI         281  AGGACCC              [GA]G^G[AT]CC[CT]
             2739  AGGACCT             
SacII           3  CCGCGG               CCGC^GG
              246  CCGCGG              
SspBI        1833  TGTACA               T^GTACA
             2193  TGTACA              
Van91I        894  CCAAGTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2474  CCAATCCTTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AflIII      
AluI        AlwI        ApoI        AvaII       BanII       
BglII       BmyI        BpmI        BsaAI       BsaBI       
BsaJI       BsiYI       BslI        BsmAI       BsmI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       CfoI        Cfr13I      DdeI        
DpnII       DraI        DsaI        EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        MaeII       
MaeIII      MamI        MboI        MboII       MfeI        
MflI        MnlI        MseI        MslI        MspA1I      
MunI        MvaI        MvnI        MwoI        NdeII       
NlaIII      NlaIV       NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII