##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccI AclI AflII Aor51HI
AscI AseI AsnI Asp700I Asp718I
BalI BciVI BclI BfrI BglII
BsaBI BsaI BsmI BspDI BsrBI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DrdI Eco47III EcoRI EcoRV
EcoT22I FbaI HincII HindII HpaI
KpnI KspI MamI MluNI MscI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SfuI SnaBI
SpeI SphI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 899 TCCGGA T^CCGGA
AcsI 2416 AAATTC [GA]^AATT[CT]
AgeI 3434 ACCGGT A^CCGGT
AhdI 1151 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 333 GTGCAC G^TGCAC
ApaLI 333 GTGCAC G^TGCAC
ApoI 2416 AAATTC [GA]^AATT[CT]
AspEI 1151 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2121 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 418 GGCGCC GGCGC^C
BbrPI 2944 CACGTG CAC^GTG
BsaAI 2944 CACGTG [CT]AC^GT[GA]
BseAI 899 TCCGGA T^CCGGA
BsiWI 2437 CGTACG C^GTACG
BsmBI 1903 CGTCTC CGTCTC(1/5)
Bsp1407I 1355 TGTACA T^GTACA
BspEI 899 TCCGGA T^CCGGA
BspHI 1698 TCATGA T^CATGA
BspLU11I 1399 ACATGT A^CATGT
BsrDI 972 CATTGC GCAATG(2/0)
BsrGI 1355 TGTACA T^GTACA
BstEII 2874 GGTGACC G^GT[ACGT]ACC
BstPI 2874 GGTGACC G^GT[ACGT]ACC
Eam1105I 1151 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1531 CTCTTC CTCTTC(1/4)
EcoO65I 2874 GGTGACC G^GT[ACGT]ACC
FseI 167 GGCCGGCC GGCCGG^CC
HaeII 418 GGCGCC [GA]GCGC^[CT]
HindIII 2726 AAGCTT A^AGCTT
KasI 418 GGCGCC G^GCGCC
Ksp632I 1531 CTCTTC CTCTTC(1/4)
MfeI 733 CAATTG C^AATTG
MroI 899 TCCGGA T^CCGGA
MunI 733 CAATTG C^AATTG
NarI 418 GGCGCC GG^CGCC
PaeR7I 2206 CTCGAG C^TCGAG
PflFI 2121 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3434 ACCGGT A^CCGGT
PmaCI 2944 CACGTG CAC^GTG
PmlI 2944 CACGTG CAC^GTG
RcaI 1698 TCATGA T^CATGA
SbfI 2517 CCTGCAGG CCTGCA^GG
SexAI 2122 ACCTGGT A^CC[AT]GGT
SfiI 1257 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 418 GGCGCC GGC^GCC
SgrAI 602 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2517 CCTGCAGG CCTGCA^GG
SspBI 1355 TGTACA T^GTACA
Tth111I 2121 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 3596 CCAGGTCCATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2206 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 1801 GACGTC GACGT^C
2332 GACGTC
AocI 1681 CCTTAGG CC^T[ACGT]AGG
2757 CCTGAGG
AviII 178 TGCGCA TGC^GCA
457 TGCGCA
BamHI 1045 GGATCC G^GATCC
1460 GGATCC
BbsI 2267 GAAGAC GAAGAC(2/6)
200 GTCTTC
BglI 1258 GCCAGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2515 GCCCTGCAGGC
BlpI 308 GCTGAGC GC^T[ACGT]AGC
2912 GCTCAGC
Bpu1102I 308 GCTGAGC GC^T[ACGT]AGC
2912 GCTCAGC
BpuAI 2267 GAAGAC GAAGAC(2/6)
200 GTCTTC
BsaWI 899 TCCGGA [AT]^CCGG[AT]
3434 ACCGGT
BseRI 351 GAGGAG GAGGAG(10/8)
2583 GAGGAG
BsiEI 70 CGGCCG CG[GA][CT]^CG
166 CGGCCG
BssSI 3647 CACGAG CACGAG(-5/-1)
1988 CTCGTG
BstXI 3381 CCATCTTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3582 CCAGCAGGGTGG
BstYI 1045 GGATCC [GA]^GATC[CT]
1460 GGATCC
Bsu36I 1681 CCTTAGG CC^T[ACGT]AGG
2757 CCTGAGG
CelII 308 GCTGAGC GC^T[ACGT]AGC
2912 GCTCAGC
DraI 1614 TTTAAA TTT^AAA
1764 TTTAAA
EagI 70 CGGCCG C^GGCCG
166 CGGCCG
EclXI 70 CGGCCG C^GGCCG
166 CGGCCG
Eco52I 70 CGGCCG C^GGCCG
166 CGGCCG
Eco81I 1681 CCTTAGG CC^T[ACGT]AGG
2757 CCTGAGG
FspI 178 TGCGCA TGC^GCA
457 TGCGCA
MflI 1045 GGATCC [GA]^GATC[CT]
1460 GGATCC
MluI 706 ACGCGT A^CGCGT
2564 ACGCGT
NaeI 168 GCCGGC GCC^GGC
634 GCCGGC
NgoMIV 168 GCCGGC G^CCGGC
634 GCCGGC
SacI 1026 GAGCTC GAGCT^C
1702 GAGCTC
XhoII 1045 GGATCC [GA]^GATC[CT]
1460 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII AvrII BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BlnI BmyI BpmI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XmaCI XmaI