##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BamHI BbrPI BclI BglII
BlnI BsaAI BsaBI BseAI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI Bsu36I
ClaI CpoI DraI DrdI Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1002 GTATAC GT^[AC][GT]AC
Aor51HI 1781 AGCGCT AGC^GCT
AspHI 337 GAGCTC G[AT]GC[AT]^C
AviII 1161 TGCGCA TGC^GCA
BalI 1024 TGGCCA TGG^CCA
BbsI 166 GAAGAC GAAGAC(2/6)
BbvCI 120 CCTCAGC CCTCAGC(-5/-2)
BciVI 1666 GGATAC GTATCC(6/5)
BlpI 2133 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2133 GCTCAGC GC^T[ACGT]AGC
BpuAI 166 GAAGAC GAAGAC(2/6)
BsgI 2353 GTGCAG GTGCAG(16/14)
BsiEI 1689 CGGCCG CG[GA][CT]^CG
BsiHKAI 337 GAGCTC G[AT]GC[AT]^C
BsiWI 1196 CGTACG C^GTACG
BsmBI 2116 GAGACG CGTCTC(1/5)
BsrBI 1114 GAGCGG CCGCTC(-3/-3)
Bst1107I 1002 GTATAC GTA^TAC
BstZ17I 1002 GTATAC GTA^TAC
CelII 2133 GCTCAGC GC^T[ACGT]AGC
DraIII 554 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1689 CGGCCG C^GGCCG
EclXI 1689 CGGCCG C^GGCCG
Eco47III 1781 AGCGCT AGC^GCT
Eco52I 1689 CGGCCG C^GGCCG
FspI 1161 TGCGCA TGC^GCA
HincII 709 GTCAAC GT[CT]^[GA]AC
HindII 709 GTCAAC GT[CT]^[GA]AC
KspI 1436 CCGCGG CCGC^GG
MluNI 1024 TGGCCA TGG^CCA
MscI 1024 TGGCCA TGG^CCA
MslI 1132 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1582 CCATGG C^CATGG
SacI 337 GAGCTC GAGCT^C
SacII 1436 CCGCGG CCGC^GG
SapI 1793 GAAGAGC GCTCTTC(1/4)
SbfI 420 CCTGCAGG CCTGCA^GG
SfiI 1690 GGCCGGTAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1008 GCATGC GCATG^C
Sse8387I 420 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 2063 GAATTT [GA]^AATT[CT]
2644 GAATTT
AflII 126 CTTAAG C^TTAAG
807 CTTAAG
AhdI 223 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACGGGCGGTC
ApoI 2063 GAATTT [GA]^AATT[CT]
2644 GAATTT
AspEI 223 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACGGGCGGTC
BbeI 1246 GGCGCC GGCGC^C
1609 GGCGCC
BcgI 913 GCATGAGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2212 GCAGATCCTTCG
BfrI 126 CTTAAG C^TTAAG
807 CTTAAG
BsaWI 1670 ACCGGA [AT]^CCGG[AT]
2112 ACCGGA
BspMI 2366 ACCTGC ACCTGC(4/8)
616 GCAGGT
Eam1105I 223 GACCAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1422 GACGGGCGGTC
EcoNI 753 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2164 CCTGGACAAGG
KasI 1246 GGCGCC G^GCGCC
1609 GGCGCC
NaeI 958 GCCGGC GCC^GGC
1965 GCCGGC
NarI 1246 GGCGCC GG^CGCC
1609 GGCGCC
NgoMIV 958 GCCGGC G^CCGGC
1965 GCCGGC
PvuII 523 CAGCTG CAG^CTG
610 CAGCTG
SfoI 1246 GGCGCC GGC^GCC
1609 GGCGCC
SmaI 1347 CCCGGG CCC^GGG
1416 CCCGGG
TaqI 540 TCGA T^CGA
1372 TCGA
Tsp509I 2064 AATT ^AATT
2645 AATT
TthHB8I 540 TCGA T^CGA
1372 TCGA
XmaCI 1347 CCCGGG C^CCGGG
1416 CCCGGG
XmaI 1347 CCCGGG C^CCGGG
1416 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I TspRI Van91I XcmI
XhoII