################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## AccIII AclI AcsI AflII AflIII Alw44I ApaLI ApoI AscI Asp700I AvrII BamHI BfaI BfrI BlnI BsaBI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrGI BstBI CpoI DraI DraIII EcoRI EcoT22I HindIII HpaI MaeI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshAI Psp1406I PvuI RsrII SbfI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 787 GGTACC G^GTACC AgeI 1368 ACCGGT A^CCGGT AhdI 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 3451 ATTAAT AT^TAAT AsnI 3451 ATTAAT AT^TAAT Asp718I 787 GGTACC G^GTACC AspEI 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 2886 CACGTG CAC^GTG BclI 1363 TGATCA T^GATCA BsaAI 2886 CACGTG [CT]AC^GT[GA] BsaI 2752 GAGACC GGTCTC(1/5) BsmI 3217 GAATGC GAATGC(1/-1) BspDI 2814 ATCGAT AT^CGAT BspHI 2017 TCATGA T^CATGA BsrDI 1106 CATTGC GCAATG(2/0) Bst1107I 2286 GTATAC GTA^TAC BstZ17I 2286 GTATAC GTA^TAC ClaI 2814 ATCGAT AT^CGAT DrdI 387 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 1363 TGATCA T^GATCA HincII 930 GTCGAC GT[CT]^[GA]AC HindII 930 GTCGAC GT[CT]^[GA]AC KpnI 787 GGTACC GGTAC^C MseI 3452 TTAA T^TAA PinAI 1368 ACCGGT A^CCGGT PmaCI 2886 CACGTG CAC^GTG PmlI 2886 CACGTG CAC^GTG PshBI 3451 ATTAAT AT^TAAT RcaI 2017 TCATGA T^CATGA SacI 987 GAGCTC GAGCT^C SalI 930 GTCGAC G^TCGAC SapI 2624 GCTCTTC GCTCTTC(1/4) ScaI 2949 AGTACT AGT^ACT SexAI 2713 ACCTGGT A^CC[AT]GGT SfiI 2201 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1425 CTCAAG C^T[CT][GA]AG TfiI 2473 GATTC G^A[AT]TC Tru9I 3452 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbeI 2233 GGCGCC GGCGC^C 2301 GGCGCC BbsI 2129 GAAGAC GAAGAC(2/6) 3353 GAAGAC BbvCI 1814 CCTCAGC CCTCAGC(-5/-2) 2493 GCTGAGG BcgI 3010 CGAGGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2806 GCAACCGCATCG BciVI 1854 GTATCC GTATCC(6/5) 3206 GTATCC BglII 1195 AGATCT A^GATCT 2332 AGATCT BlpI 1124 GCTCAGC GC^T[ACGT]AGC 3139 GCTCAGC Bpu1102I 1124 GCTCAGC GC^T[ACGT]AGC 3139 GCTCAGC BpuAI 2129 GAAGAC GAAGAC(2/6) 3353 GAAGAC BsmBI 97 CGTCTC CGTCTC(1/5) 861 CGTCTC BssHII 125 GCGCGC G^CGCGC 127 GCGCGC CelII 1124 GCTCAGC GC^T[ACGT]AGC 3139 GCTCAGC EagI 742 CGGCCG C^GGCCG 1148 CGGCCG EclXI 742 CGGCCG C^GGCCG 1148 CGGCCG Eco52I 742 CGGCCG C^GGCCG 1148 CGGCCG EcoRV 1134 GATATC GAT^ATC 2817 GATATC FseI 739 GGCCGGCC GGCCGG^CC 1460 GGCCGGCC KasI 2233 GGCGCC G^GCGCC 2301 GGCGCC NarI 2233 GGCGCC GG^CGCC 2301 GGCGCC NcoI 1095 CCATGG C^CATGG 3423 CCATGG SfoI 2233 GGCGCC GGC^GCC 2301 GGCGCC Tsp509I 286 AATT ^AATT 3450 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AatII AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI AspHI AspI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StuI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI