##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
Alw44I ApaLI ApoI AscI Asp700I
AvrII BamHI BfaI BfrI BlnI
BsaBI BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrGI BstBI CpoI DraI
DraIII EcoRI EcoT22I HindIII HpaI
MaeI MamI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI Psp1406I PvuI RsrII SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 787 GGTACC G^GTACC
AgeI 1368 ACCGGT A^CCGGT
AhdI 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3451 ATTAAT AT^TAAT
AsnI 3451 ATTAAT AT^TAAT
Asp718I 787 GGTACC G^GTACC
AspEI 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 2886 CACGTG CAC^GTG
BclI 1363 TGATCA T^GATCA
BsaAI 2886 CACGTG [CT]AC^GT[GA]
BsaI 2752 GAGACC GGTCTC(1/5)
BsmI 3217 GAATGC GAATGC(1/-1)
BspDI 2814 ATCGAT AT^CGAT
BspHI 2017 TCATGA T^CATGA
BsrDI 1106 CATTGC GCAATG(2/0)
Bst1107I 2286 GTATAC GTA^TAC
BstZ17I 2286 GTATAC GTA^TAC
ClaI 2814 ATCGAT AT^CGAT
DrdI 387 GACGGGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2526 GACTGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1363 TGATCA T^GATCA
HincII 930 GTCGAC GT[CT]^[GA]AC
HindII 930 GTCGAC GT[CT]^[GA]AC
KpnI 787 GGTACC GGTAC^C
MseI 3452 TTAA T^TAA
PinAI 1368 ACCGGT A^CCGGT
PmaCI 2886 CACGTG CAC^GTG
PmlI 2886 CACGTG CAC^GTG
PshBI 3451 ATTAAT AT^TAAT
RcaI 2017 TCATGA T^CATGA
SacI 987 GAGCTC GAGCT^C
SalI 930 GTCGAC G^TCGAC
SapI 2624 GCTCTTC GCTCTTC(1/4)
ScaI 2949 AGTACT AGT^ACT
SexAI 2713 ACCTGGT A^CC[AT]GGT
SfiI 2201 GGCCGAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1425 CTCAAG C^T[CT][GA]AG
TfiI 2473 GATTC G^A[AT]TC
Tru9I 3452 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BbeI 2233 GGCGCC GGCGC^C
2301 GGCGCC
BbsI 2129 GAAGAC GAAGAC(2/6)
3353 GAAGAC
BbvCI 1814 CCTCAGC CCTCAGC(-5/-2)
2493 GCTGAGG
BcgI 3010 CGAGGGCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2806 GCAACCGCATCG
BciVI 1854 GTATCC GTATCC(6/5)
3206 GTATCC
BglII 1195 AGATCT A^GATCT
2332 AGATCT
BlpI 1124 GCTCAGC GC^T[ACGT]AGC
3139 GCTCAGC
Bpu1102I 1124 GCTCAGC GC^T[ACGT]AGC
3139 GCTCAGC
BpuAI 2129 GAAGAC GAAGAC(2/6)
3353 GAAGAC
BsmBI 97 CGTCTC CGTCTC(1/5)
861 CGTCTC
BssHII 125 GCGCGC G^CGCGC
127 GCGCGC
CelII 1124 GCTCAGC GC^T[ACGT]AGC
3139 GCTCAGC
EagI 742 CGGCCG C^GGCCG
1148 CGGCCG
EclXI 742 CGGCCG C^GGCCG
1148 CGGCCG
Eco52I 742 CGGCCG C^GGCCG
1148 CGGCCG
EcoRV 1134 GATATC GAT^ATC
2817 GATATC
FseI 739 GGCCGGCC GGCCGG^CC
1460 GGCCGGCC
KasI 2233 GGCGCC G^GCGCC
2301 GGCGCC
NarI 2233 GGCGCC GG^CGCC
2301 GGCGCC
NcoI 1095 CCATGG C^CATGG
3423 CCATGG
SfoI 2233 GGCGCC GGC^GCC
2301 GGCGCC
Tsp509I 286 AATT ^AATT
3450 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AatII AccI AccII AciI AcyI
AfaI AluI AlwI AlwNI AocI
Aor51HI ApaI AspHI AspI AvaI
AvaII AviII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XcmI XhoII XmaCI XmaI