################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AspI AvaI AvrII BamHI BbeI BcgI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DsaI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV FbaI FseI HaeII HgaI Hin1I KasI KspI MamI MluI MroI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PvuI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 1968 AACGTT AA^CGTT AflIII 1237 ACATGT A^C[GA][CT]GT AhdI 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 79 GTGCTC G[AT]GC[AT]^C AviII 1510 TGCGCA TGC^GCA BanII 1809 GAGCCC G[GA]GC[CT]^C BbrPI 3437 CACGTG CAC^GTG BbsI 17 GTCTTC GAAGAC(2/6) BbvCI 125 CCTCAGC CCTCAGC(-5/-2) BciVI 2007 GGATAC GTATCC(6/5) BcnI 656 CCGGG CC^[GC]GG BglII 670 AGATCT A^GATCT BpuAI 17 GTCTTC GAAGAC(2/6) BsaAI 3437 CACGTG [CT]AC^GT[GA] BsiHKAI 79 GTGCTC G[AT]GC[AT]^C BsmI 3321 GAATGC GAATGC(1/-1) Bsp1407I 3367 TGTACA T^GTACA BspLU11I 1237 ACATGT A^CATGT BsrGI 3367 TGTACA T^GTACA BstEII 484 GGTGACC G^GT[ACGT]ACC BstPI 484 GGTGACC G^GT[ACGT]ACC BstXI 1216 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 1511 GCGC GCG^C DraII 1032 AGGACCT [GA]G^G[ACGT]CC[CT] DraIII 112 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 385 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1779 CTCTTC CTCTTC(1/4) EcoO109I 1032 AGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 484 GGTGACC G^GT[ACGT]ACC FspI 1510 TGCGCA TGC^GCA HhaI 1511 GCGC GCG^C HinP1I 1511 GCGC G^CGC HindIII 419 AAGCTT A^AGCTT Ksp632I 1779 CTCTTC CTCTTC(1/4) MfeI 1052 CAATTG C^AATTG MunI 1052 CAATTG C^AATTG NciI 656 CCGGG CC^[GC]GG PmaCI 3437 CACGTG CAC^GTG PmlI 3437 CACGTG CAC^GTG PpuMI 1032 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 1968 AACGTT AA^CGTT SexAI 2056 ACCAGGT A^CC[AT]GGT SpeI 3459 ACTAGT A^CTAGT SspBI 3367 TGTACA T^GTACA TaqI 718 TCGA T^CGA TthHB8I 718 TCGA T^CGA XbaI 1279 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### Acc65I 549 GGTACC G^GTACC 2060 GGTACC AccI 2556 GTAGAC GT^[AC][GT]AC 2676 GTAGAC Asp718I 549 GGTACC G^GTACC 2060 GGTACC BalI 1557 TGGCCA TGG^CCA 2252 TGGCCA BmyI 79 GTGCTC G[AGT]GC[ACT]^C 1809 GAGCCC BpmI 965 CTCCAG CTGGAG(16/14) 326 CTGGAG BsaI 1648 GAGACC GGTCTC(1/5) 204 GGTCTC BsaJI 85 CCTTGG C^C[ACGT][ACGT]GG 319 CCAAGG Bsp1286I 79 GTGCTC G[AGT]GC[ACT]^C 1809 GAGCCC BstAPI 2260 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3002 GCACTTCCTGC BstYI 670 AGATCT [GA]^GATC[CT] 1550 GGATCT EcoT14I 85 CCTTGG C^C[AT][AT]GG 319 CCAAGG EcoT22I 1995 ATGCAT ATGCA^T 2620 ATGCAT FauI 537 CCCGC CCCGC(4/6) 159 GCGGG HapII 656 CCGG C^CGG 2288 CCGG HpaI 1591 GTTAAC GTT^AAC 3161 GTTAAC HpaII 656 CCGG C^CGG 2288 CCGG KpnI 549 GGTACC GGTAC^C 2060 GGTACC MflI 670 AGATCT [GA]^GATC[CT] 1550 GGATCT MluNI 1557 TGGCCA TGG^CCA 2252 TGGCCA MscI 1557 TGGCCA TGG^CCA 2252 TGGCCA MspA1I 1246 CAGCTG C[AC]G^C[GT]G 1514 CAGCTG MspI 656 CCGG C^CGG 2288 CCGG NdeI 2220 CATATG CA^TATG 3045 CATATG NsiI 1995 ATGCAT ATGCA^T 2620 ATGCAT PflMI 319 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 967 CCAGAAACTGG PleI 2340 GACTC GAGTC(4/5) 2362 GACTC PvuII 1246 CAGCTG CAG^CTG 1514 CAGCTG ScaI 1218 AGTACT AGT^ACT 1529 AGTACT SmlI 1118 CTCAAG C^T[CT][GA]AG 2929 CTTGAG SphI 1368 GCATGC GCATG^C 1507 GCATGC StyI 85 CCTTGG C^C[AT][AT]GG 319 CCAAGG Van91I 319 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 967 CCAGAAACTGG XcmI 824 CCAAGACTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2228 CCATCATTCATCTGG XhoII 670 AGATCT [GA]^GATC[CT] 1550 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaII BanI BbvI BfaI BsgI BsiYI BslI BsmAI BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EaeI EcoRII Fnu4HI FokI HaeIII HincII HindII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeII NlaIII NlaIV NspI PshBI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI