##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI AscI AspI AvaI AvrII
BamHI BbeI BcgI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV FbaI FseI
HaeII HgaI Hin1I KasI KspI
MamI MluI MroI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I StuI SwaI Tth111I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AclI 1968 AACGTT AA^CGTT
AflIII 1237 ACATGT A^C[GA][CT]GT
AhdI 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 79 GTGCTC G[AT]GC[AT]^C
AviII 1510 TGCGCA TGC^GCA
BanII 1809 GAGCCC G[GA]GC[CT]^C
BbrPI 3437 CACGTG CAC^GTG
BbsI 17 GTCTTC GAAGAC(2/6)
BbvCI 125 CCTCAGC CCTCAGC(-5/-2)
BciVI 2007 GGATAC GTATCC(6/5)
BcnI 656 CCGGG CC^[GC]GG
BglII 670 AGATCT A^GATCT
BpuAI 17 GTCTTC GAAGAC(2/6)
BsaAI 3437 CACGTG [CT]AC^GT[GA]
BsiHKAI 79 GTGCTC G[AT]GC[AT]^C
BsmI 3321 GAATGC GAATGC(1/-1)
Bsp1407I 3367 TGTACA T^GTACA
BspLU11I 1237 ACATGT A^CATGT
BsrGI 3367 TGTACA T^GTACA
BstEII 484 GGTGACC G^GT[ACGT]ACC
BstPI 484 GGTGACC G^GT[ACGT]ACC
BstXI 1216 CCAGTACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1511 GCGC GCG^C
DraII 1032 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 112 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 385 GACTGCAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 66 GACAGGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1779 CTCTTC CTCTTC(1/4)
EcoO109I 1032 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 484 GGTGACC G^GT[ACGT]ACC
FspI 1510 TGCGCA TGC^GCA
HhaI 1511 GCGC GCG^C
HinP1I 1511 GCGC G^CGC
HindIII 419 AAGCTT A^AGCTT
Ksp632I 1779 CTCTTC CTCTTC(1/4)
MfeI 1052 CAATTG C^AATTG
MunI 1052 CAATTG C^AATTG
NciI 656 CCGGG CC^[GC]GG
PmaCI 3437 CACGTG CAC^GTG
PmlI 3437 CACGTG CAC^GTG
PpuMI 1032 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1968 AACGTT AA^CGTT
SexAI 2056 ACCAGGT A^CC[AT]GGT
SpeI 3459 ACTAGT A^CTAGT
SspBI 3367 TGTACA T^GTACA
TaqI 718 TCGA T^CGA
TthHB8I 718 TCGA T^CGA
XbaI 1279 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 549 GGTACC G^GTACC
2060 GGTACC
AccI 2556 GTAGAC GT^[AC][GT]AC
2676 GTAGAC
Asp718I 549 GGTACC G^GTACC
2060 GGTACC
BalI 1557 TGGCCA TGG^CCA
2252 TGGCCA
BmyI 79 GTGCTC G[AGT]GC[ACT]^C
1809 GAGCCC
BpmI 965 CTCCAG CTGGAG(16/14)
326 CTGGAG
BsaI 1648 GAGACC GGTCTC(1/5)
204 GGTCTC
BsaJI 85 CCTTGG C^C[ACGT][ACGT]GG
319 CCAAGG
Bsp1286I 79 GTGCTC G[AGT]GC[ACT]^C
1809 GAGCCC
BstAPI 2260 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3002 GCACTTCCTGC
BstYI 670 AGATCT [GA]^GATC[CT]
1550 GGATCT
EcoT14I 85 CCTTGG C^C[AT][AT]GG
319 CCAAGG
EcoT22I 1995 ATGCAT ATGCA^T
2620 ATGCAT
FauI 537 CCCGC CCCGC(4/6)
159 GCGGG
HapII 656 CCGG C^CGG
2288 CCGG
HpaI 1591 GTTAAC GTT^AAC
3161 GTTAAC
HpaII 656 CCGG C^CGG
2288 CCGG
KpnI 549 GGTACC GGTAC^C
2060 GGTACC
MflI 670 AGATCT [GA]^GATC[CT]
1550 GGATCT
MluNI 1557 TGGCCA TGG^CCA
2252 TGGCCA
MscI 1557 TGGCCA TGG^CCA
2252 TGGCCA
MspA1I 1246 CAGCTG C[AC]G^C[GT]G
1514 CAGCTG
MspI 656 CCGG C^CGG
2288 CCGG
NdeI 2220 CATATG CA^TATG
3045 CATATG
NsiI 1995 ATGCAT ATGCA^T
2620 ATGCAT
PflMI 319 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
967 CCAGAAACTGG
PleI 2340 GACTC GAGTC(4/5)
2362 GACTC
PvuII 1246 CAGCTG CAG^CTG
1514 CAGCTG
ScaI 1218 AGTACT AGT^ACT
1529 AGTACT
SmlI 1118 CTCAAG C^T[CT][GA]AG
2929 CTTGAG
SphI 1368 GCATGC GCATG^C
1507 GCATGC
StyI 85 CCTTGG C^C[AT][AT]GG
319 CCAAGG
Van91I 319 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
967 CCAGAAACTGG
XcmI 824 CCAAGACTACATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2228 CCATCATTCATCTGG
XhoII 670 AGATCT [GA]^GATC[CT]
1550 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI Asp700I
AvaII BanI BbvI BfaI BsgI
BsiYI BslI BsmAI BspHI BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EaeI EcoRII Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII NlaIV NspI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI