##################################
# NO CUT ENZYMES ( 45 enzymes) #
##################################
Acc65I AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp718I AspEI
AviII AvrII BbeI BfrI BlnI
BsiWI BssHII Bst1107I BstZ17I CpoI
EagI Eam1105I EclXI Eco47III Eco52I
FspI KasI KpnI MfeI MluI
MunI NarI NotI NruI PacI
PinAI PmeI PshBI RsrII SfiI
SfoI SnaBI SpeI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1627 GACGTC GACGT^C
AclI 633 AACGTT AA^CGTT
AcyI 1627 GACGTC G[GA]^CG[CT]C
BanI 6421 GGTGCC G^G[CT][GA]CC
BbrPI 756 CACGTG CAC^GTG
BsaBI 6764 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1627 GACGTC G[GA]^CG[CT]C
Bsp1407I 2960 TGTACA T^GTACA
BsrGI 2960 TGTACA T^GTACA
BssSI 9900 CACGAG CACGAG(-5/-1)
BstBI 1221 TTCGAA TT^CGAA
EcoT22I 6048 ATGCAT ATGCA^T
FseI 5270 GGCCGGCC GGCCGG^CC
Hin1I 1627 GACGTC G[GA]^CG[CT]C
KspI 4929 CCGCGG CCGC^GG
MamI 6764 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 12 CATATG CA^TATG
NsiI 6048 ATGCAT ATGCA^T
NspV 1221 TTCGAA TT^CGAA
PmaCI 756 CACGTG CAC^GTG
PmlI 756 CACGTG CAC^GTG
PshAI 9825 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 633 AACGTT AA^CGTT
PvuI 1977 CGATCG CGAT^CG
SacII 4929 CCGCGG CCGC^GG
SalI 1552 GTCGAC G^TCGAC
SbfI 9249 CCTGCAGG CCTGCA^GG
SfuI 1221 TTCGAA TT^CGAA
SgrAI 6571 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 9249 CCTGCAGG CCTGCA^GG
SspBI 2960 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 2027 GGGCCC GGGCC^C
2881 GGGCCC
AspI 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
9291 GACGAAGTC
BciVI 3840 GGATAC GTATCC(6/5)
1820 GTATCC
BsmBI 5917 GAGACG CGTCTC(1/5)
8314 GAGACG
BspLU11I 9068 ACATGT A^CATGT
9476 ACATGT
BstAPI 3211 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
6453 GCAGAAGCTGC
BstEII 3492 GGTAACC G^GT[ACGT]ACC
9486 GGTGACC
BstPI 3492 GGTAACC G^GT[ACGT]ACC
9486 GGTGACC
DrdI 6655 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
8698 GACTTCCAGGTC
EcoO65I 3492 GGTAACC G^GT[ACGT]ACC
9486 GGTGACC
EcoRV 4018 GATATC GAT^ATC
7870 GATATC
HindIII 2938 AAGCTT A^AGCTT
9274 AAGCTT
HpaI 2277 GTTAAC GTT^AAC
2853 GTTAAC
NaeI 2035 GCCGGC GCC^GGC
5271 GCCGGC
NgoMIV 2035 GCCGGC G^CCGGC
5271 GCCGGC
NheI 1594 GCTAGC G^CTAGC
4440 GCTAGC
PaeR7I 92 CTCGAG C^TCGAG
9062 CTCGAG
PflFI 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
9291 GACGAAGTC
SapI 5070 GAAGAGC GCTCTTC(1/4)
2451 GCTCTTC
ScaI 5294 AGTACT AGT^ACT
7562 AGTACT
SphI 51 GCATGC GCATG^C
1782 GCATGC
SspI 4456 AATATT AAT^ATT
10180 AATATT
Tth111I 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
9291 GACGAAGTC
XhoI 92 CTCGAG C^TCGAG
9062 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 158 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BamHI
BanII BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BglII
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I ClaI DdeI DpnII DraI
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI XmnI