################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## Acc65I AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BbeI BfrI BlnI BsiWI BssHII Bst1107I BstZ17I CpoI EagI Eam1105I EclXI Eco47III Eco52I FspI KasI KpnI MfeI MluI MunI NarI NotI NruI PacI PinAI PmeI PshBI RsrII SfiI SfoI SnaBI SpeI SwaI XbaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 1627 GACGTC GACGT^C AclI 633 AACGTT AA^CGTT AcyI 1627 GACGTC G[GA]^CG[CT]C BanI 6421 GGTGCC G^G[CT][GA]CC BbrPI 756 CACGTG CAC^GTG BsaBI 6764 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1627 GACGTC G[GA]^CG[CT]C Bsp1407I 2960 TGTACA T^GTACA BsrGI 2960 TGTACA T^GTACA BssSI 9900 CACGAG CACGAG(-5/-1) BstBI 1221 TTCGAA TT^CGAA EcoT22I 6048 ATGCAT ATGCA^T FseI 5270 GGCCGGCC GGCCGG^CC Hin1I 1627 GACGTC G[GA]^CG[CT]C KspI 4929 CCGCGG CCGC^GG MamI 6764 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NdeI 12 CATATG CA^TATG NsiI 6048 ATGCAT ATGCA^T NspV 1221 TTCGAA TT^CGAA PmaCI 756 CACGTG CAC^GTG PmlI 756 CACGTG CAC^GTG PshAI 9825 GACCCCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 633 AACGTT AA^CGTT PvuI 1977 CGATCG CGAT^CG SacII 4929 CCGCGG CCGC^GG SalI 1552 GTCGAC G^TCGAC SbfI 9249 CCTGCAGG CCTGCA^GG SfuI 1221 TTCGAA TT^CGAA SgrAI 6571 CACCGGCG C[GA]^CCGG[CT]G Sse8387I 9249 CCTGCAGG CCTGCA^GG SspBI 2960 TGTACA T^GTACA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### ApaI 2027 GGGCCC GGGCC^C 2881 GGGCCC AspI 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 9291 GACGAAGTC BciVI 3840 GGATAC GTATCC(6/5) 1820 GTATCC BsmBI 5917 GAGACG CGTCTC(1/5) 8314 GAGACG BspLU11I 9068 ACATGT A^CATGT 9476 ACATGT BstAPI 3211 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 6453 GCAGAAGCTGC BstEII 3492 GGTAACC G^GT[ACGT]ACC 9486 GGTGACC BstPI 3492 GGTAACC G^GT[ACGT]ACC 9486 GGTGACC DrdI 6655 GACGAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 8698 GACTTCCAGGTC EcoO65I 3492 GGTAACC G^GT[ACGT]ACC 9486 GGTGACC EcoRV 4018 GATATC GAT^ATC 7870 GATATC HindIII 2938 AAGCTT A^AGCTT 9274 AAGCTT HpaI 2277 GTTAAC GTT^AAC 2853 GTTAAC NaeI 2035 GCCGGC GCC^GGC 5271 GCCGGC NgoMIV 2035 GCCGGC G^CCGGC 5271 GCCGGC NheI 1594 GCTAGC G^CTAGC 4440 GCTAGC PaeR7I 92 CTCGAG C^TCGAG 9062 CTCGAG PflFI 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 9291 GACGAAGTC SapI 5070 GAAGAGC GCTCTTC(1/4) 2451 GCTCTTC ScaI 5294 AGTACT AGT^ACT 7562 AGTACT SphI 51 GCATGC GCATG^C 1782 GCATGC SspI 4456 AATATT AAT^ATT 10180 AATATT Tth111I 7021 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 9291 GACGAAGTC XhoI 92 CTCGAG C^TCGAG 9062 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 158 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcgI BclI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI